Restriction map for pF1KB6003
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccII       AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BlnI        BsaBI       BsaI        BsiEI       
BsiWI       BsmBI       BspDI       BspLU11I    BsrBI       
BssHII      BstAPI      BstBI       BstUI       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      FauI        FbaI        
FseI        FspI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SwaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        3207  GACGTC               GACGT^C
AccIII       1575  TCCGGA               T^CCGGA
AclI          808  AACGTT               AA^CGTT
AflII        1170  CTTAAG               C^TTAAG
AflIII       2700  ACGTGT               A^C[GA][CT]GT
Alw44I       2549  GTGCAC               G^TGCAC
AocI          359  CCTTAGG              CC^T[ACGT]AGG
ApaLI        2549  GTGCAC               G^TGCAC
Asp700I      3429  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         3517  CCCGAG               C^[CT]CG[GA]G
BamHI        1595  GGATCC               G^GATCC
BanII        2338  GAGCCC               G[GA]GC[CT]^C
BbvCI          66  CCTCAGC              CCTCAGC(-5/-2)
BcnI         2874  CCCGG                CC^[GC]GG
BfrI         1170  CTTAAG               C^TTAAG
BglI         3391  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1057  AGATCT               A^GATCT
BlpI         1048  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1048  GCTCAGC              GC^T[ACGT]AGC
BsaAI        3454  TACGTG               [CT]AC^GT[GA]
BsaWI        1575  TCCGGA               [AT]^CCGG[AT]
BseAI        1575  TCCGGA               T^CCGGA
BsmI         2881  GAATGC               GAATGC(1/-1)
BsoBI        3517  CCCGAG               C^[CT]CG[GA]G
BspEI        1575  TCCGGA               T^CCGGA
BspMI         156  GCAGGT               ACCTGC(4/8)
BsrFI          26  GCCGGT               [GA]^CCGG[CT]
Bst1107I      506  GTATAC               GTA^TAC
BstEII        634  GGTCACC              G^GT[ACGT]ACC
BstPI         634  GGTCACC              G^GT[ACGT]ACC
BstZ17I       506  GTATAC               GTA^TAC
Bsu36I        359  CCTTAGG              CC^T[ACGT]AGG
CelII        1048  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         26  GCCGGT               [GA]^CCGG[CT]
DraIII        937  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3395  CCATGG               C^C[GA][CT]GG
Eco81I        359  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       634  GGTCACC              G^GT[ACGT]ACC
HaeII        3584  GGCGCT               [GA]GCGC^[CT]
MroI         1575  TCCGGA               T^CCGGA
NciI         2874  CCCGG                CC^[GC]GG
NcoI         3395  CCATGG               C^CATGG
Psp1406I      808  AACGTT               AA^CGTT
PstI         2649  CTGCAG               CTGCA^G
SapI         2335  GAAGAGC              GCTCTTC(1/4)
SphI         1005  GCATGC               GCATG^C
SspI         2297  AATATT               AAT^ATT
TaqI         3275  TCGA                 T^CGA
TthHB8I      3275  TCGA                 T^CGA
XmnI         3429  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          506  GTATAC               GT^[AC][GT]AC
             3451  GTCTAC              
AspI          193  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3376  GACGCCGTC           
BanI          354  GGTGCC               G^G[CT][GA]CC
             3172  GGCACC              
BstXI        3054  CCACTTGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3185  CCAAAGTTTTGG        
CfoI         3304  GCGC                 GCG^C
             3585  GCGC                
EcoNI        2530  CCTTTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3282  CCTCCGCCAGG         
EcoRI        2385  GAATTC               G^AATTC
             2463  GAATTC              
EcoRV        1303  GATATC               GAT^ATC
             1399  GATATC              
EcoT22I      1469  ATGCAT               ATGCA^T
             3040  ATGCAT              
HhaI         3304  GCGC                 GCG^C
             3585  GCGC                
HinP1I       3304  GCGC                 G^CGC
             3585  GCGC                
HindIII      2039  AAGCTT               A^AGCTT
             3508  AAGCTT              
HpaI          226  GTTAAC               GTT^AAC
             3108  GTTAAC              
MspA1I       2196  CCGCTG               C[AC]G^C[GT]G
             2895  CCGCTG              
NsiI         1469  ATGCAT               ATGCA^T
             3040  ATGCAT              
NspI          510  ACATGC               [GA]CATG^[CT]
             1005  GCATGC              
PflFI         193  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3376  GACGCCGTC           
PshAI         412  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              628  GACATAGGTC          
Tth111I       193  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3376  GACGCCGTC           
XbaI          489  TCTAGA               T^CTAGA
             2574  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
AspHI       AvaII       BalI        BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BspHI       BsrDI       BsrGI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PshBI       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XhoII       
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