Restriction map for pF1KB5645
##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

Acc65I      AclI        AflII       Aor51HI     AscI        
AseI        AsnI        Asp718I     AspI        AvrII       
BfrI        BlnI        BlpI        Bpu1102I    BsaBI       
BsiWI       BspHI       BssHII      Bst1107I    BstBI       
BstZ17I     CelII       CpoI        DrdI        Eco47III    
EcoNI       EcoRV       FseI        HindIII     HpaI        
KpnI        KspI        MamI        MluI        NdeI        
NheI        NruI        NspV        PacI        PaeR7I      
PflFI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         538  GACGTC               GACGT^C
AccIII       2523  TCCGGA               T^CCGGA
AflIII       1348  ACATGT               A^C[GA][CT]GT
AgeI         1817  ACCGGT               A^CCGGT
AhdI           17  GACTAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1755  GTGCAC               G^TGCAC
ApaLI        1755  GTGCAC               G^TGCAC
Asp700I      3306  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI          17  GACTAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1373  GGATCC               G^GATCC
BbeI          134  GGCGCC               GGCGC^C
BbrPI         415  CACGTG               CAC^GTG
BclI         1364  TGATCA               T^GATCA
BglI         2638  GCCAACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1616  AGATCT               A^GATCT
BsaAI         415  CACGTG               [CT]AC^GT[GA]
BseAI        2523  TCCGGA               T^CCGGA
BsiEI        1206  CGGCCG               CG[GA][CT]^CG
Bsp1407I      500  TGTACA               T^GTACA
BspDI        2824  ATCGAT               AT^CGAT
BspEI        2523  TCCGGA               T^CCGGA
BspLU11I     1348  ACATGT               A^CATGT
BsrGI         500  TGTACA               T^GTACA
BstEII       1493  GGTCACC              G^GT[ACGT]ACC
BstPI        1493  GGTCACC              G^GT[ACGT]ACC
BstXI         563  CCAGAGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         2824  ATCGAT               AT^CGAT
DraI         1294  TTTAAA               TTT^AAA
EagI         1206  CGGCCG               C^GGCCG
Eam1105I       17  GACTAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1206  CGGCCG               C^GGCCG
Eco52I       1206  CGGCCG               C^GGCCG
EcoO65I      1493  GGTCACC              G^GT[ACGT]ACC
EcoRI        1193  GAATTC               G^AATTC
EcoT22I        37  ATGCAT               ATGCA^T
FbaI         1364  TGATCA               T^GATCA
HaeII         134  GGCGCC               [GA]GCGC^[CT]
KasI          134  GGCGCC               G^GCGCC
MroI         2523  TCCGGA               T^CCGGA
NaeI         1672  GCCGGC               GCC^GGC
NarI          134  GGCGCC               GG^CGCC
NgoMIV       1672  GCCGGC               G^CCGGC
NotI         1205  GCGGCCGC             GC^GGCCGC
NsiI           37  ATGCAT               ATGCA^T
PinAI        1817  ACCGGT               A^CCGGT
PmaCI         415  CACGTG               CAC^GTG
PmlI          415  CACGTG               CAC^GTG
SapI          459  GAAGAGC              GCTCTTC(1/4)
SfoI          134  GGCGCC               GGC^GCC
SgrAI        1816  CACCGGTG             C[GA]^CCGG[CT]G
SmaI          299  CCCGGG               CCC^GGG
SmlI         3461  CTCAAG               C^T[CT][GA]AG
SspBI         500  TGTACA               T^GTACA
XmaCI         299  CCCGGG               C^CCGGG
XmaI          299  CCCGGG               C^CCGGG
XmnI         3306  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         2446  GTCTAC               GT^[AC][GT]AC
             3437  GTAGAC              
AccII        1204  CGCG                 CG^CG
             1433  CGCG                
AcsI         1193  GAATTC               [GA]^AATT[CT]
             2237  AAATTC              
AocI          279  CCTGAGG              CC^T[ACGT]AGG
              678  CCTCAGG             
ApaI          150  GGGCCC               GGGCC^C
              491  GGGCCC              
ApoI         1193  GAATTC               [GA]^AATT[CT]
             2237  AAATTC              
AvaI          299  CCCGGG               C^[CT]CG[GA]G
             3174  CTCGGG              
AviII         860  TGCGCA               TGC^GCA
             3269  TGCGCA              
BbvCI         387  CCTCAGC              CCTCAGC(-5/-2)
              933  GCTGAGG             
BcgI         3057  CGATGACTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3282  GCATGACCCTCG        
BciVI        3169  GGATAC               GTATCC(6/5)
             2414  GTATCC              
BsaWI        1817  ACCGGT               [AT]^CCGG[AT]
             2523  TCCGGA              
BsoBI         299  CCCGGG               C^[CT]CG[GA]G
             3174  CTCGGG              
BspMI        1264  ACCTGC               ACCTGC(4/8)
             3224  ACCTGC              
BsrBI        1209  CCGCTC               CCGCTC(-3/-3)
              322  GAGCGG              
BsrDI        1187  GCAATG               GCAATG(2/0)
             2051  GCAATG              
BssSI        3156  CACGAG               CACGAG(-5/-1)
             2734  CTCGTG              
BstUI        1204  CGCG                 CG^CG
             1433  CGCG                
Bsu36I        279  CCTGAGG              CC^T[ACGT]AGG
              678  CCTCAGG             
EarI          459  GAAGAG               CTCTTC(1/4)
              907  GAAGAG              
Eco81I        279  CCTGAGG              CC^T[ACGT]AGG
              678  CCTCAGG             
FspI          860  TGCGCA               TGC^GCA
             3269  TGCGCA              
HincII       2848  GTTGAC               GT[CT]^[GA]AC
             2968  GTCAAC              
HindII       2848  GTTGAC               GT[CT]^[GA]AC
             2968  GTCAAC              
Ksp632I       459  GAAGAG               CTCTTC(1/4)
              907  GAAGAG              
MfeI         1640  CAATTG               C^AATTG
             2502  CAATTG              
MseI         1295  TTAA                 T^TAA
             3008  TTAA                
MunI         1640  CAATTG               C^AATTG
             2502  CAATTG              
MvnI         1204  CGCG                 CG^CG
             1433  CGCG                
SexAI         230  ACCTGGT              A^CC[AT]GGT
             2105  ACCTGGT             
TfiI         2876  GATTC                G^A[AT]TC
             2886  GATTC               
Tru9I        1295  TTAA                 T^TAA
             3008  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1286I    BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       
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