Restriction map for pF1KB5530
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        KspI        
MaeII       MfeI        MflI        MluI        MroI        
MslI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsaI        
RsrII       SacI        SacII       SalI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TspRI       Tth111I     Van91I      
XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcyI           76  GACGCC               G[GA]^CG[CT]C
AluI           86  AGCT                 AG^CT
AlwI          180  GGATC                GGATC(4/5)
AvaI          112  CCCGGG               C^[CT]CG[GA]G
BalI           65  TGGCCA               TGG^CCA
BanI          253  GGCACC               G^G[CT][GA]CC
BbsI          265  GAAGAC               GAAGAC(2/6)
BfaI          245  CTAG                 C^TAG
BpuAI         265  GAAGAC               GAAGAC(2/6)
BsaBI         351  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          76  GACGCC               G[GA]^CG[CT]C
BsmFI         118  GGGAC                GGGAC(10/14)
BsoBI         112  CCCGGG               C^[CT]CG[GA]G
BspHI          93  TCATGA               T^CATGA
BsrFI          13  GCCGGC               [GA]^CCGG[CT]
Cac8I          13  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I         13  GCCGGC               [GA]^CCGG[CT]
DpnII         181  GATC                 ^GATC
EarI          124  CTCTTC               CTCTTC(1/4)
EcoT14I       197  CCATGG               C^C[AT][AT]GG
FauI           56  CCCGC                CCCGC(4/6)
Hin1I          76  GACGCC               G[GA]^CG[CT]C
Ksp632I       124  CTCTTC               CTCTTC(1/4)
MaeI          245  CTAG                 C^TAG
MaeIII        241  GTCAC                ^GT[ACGT]AC
MamI          351  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          181  GATC                 ^GATC
MluNI          65  TGGCCA               TGG^CCA
MscI           65  TGGCCA               TGG^CCA
MseI          259  TTAA                 T^TAA
MspA1I         18  CAGCGG               C[AC]G^C[GT]G
MwoI           35  GCCAGAGCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI           13  GCCGGC               GCC^GGC
NcoI          197  CCATGG               C^CATGG
NdeII         181  GATC                 ^GATC
NgoMIV         13  GCCGGC               G^CCGGC
NlaIV         253  GGCACC               GG[ACGT]^[ACGT]CC
NspI          321  ACATGC               [GA]CATG^[CT]
RcaI           93  TCATGA               T^CATGA
SapI          123  GCTCTTC              GCTCTTC(1/4)
Sau3AI        181  GATC                 ^GATC
SfaNI         337  GATGC                GCATC(5/9)
SmaI          112  CCCGGG               CCC^GGG
StyI          197  CCATGG               C^C[AT][AT]GG
TfiI          152  GAATC                G^A[AT]TC
Tru9I         259  TTAA                 T^TAA
Tsp45I        241  GTCAC                ^GT[GC]AC
XcmI           53  CCACCCGCACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         112  CCCGGG               C^CCGGG
XmaI          112  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          43  CGCG                 CG^CG
               81  CGCG                
BbvI           17  GCAGC                GCAGC(8/12)
               84  GCAGC               
BsiYI          57  CCGCACCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              107  CCACGCCCGGG         
BslI           57  CCGCACCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              107  CCACGCCCGGG         
BstF5I         48  CATCC                GGATG(2/0)
              333  GGATG               
BstNI         235  CCAGG                CC^[AT]GG
              250  CCTGG               
BstUI          43  CGCG                 CG^CG
               81  CGCG                
CfoI           44  GCGC                 GCG^C
               82  GCGC                
DdeI          203  CTCAG                C^T[ACGT]AG
              341  CTCAG               
DsaI           62  CCGTGG               C^C[GA][CT]GG
              197  CCATGG              
EaeI           21  CGGCCA               [CT]^GGCC[GA]
               65  TGGCCA              
EcoRII        235  CCAGG                ^CC[AT]GG
              250  CCTGG               
FokI           48  CATCC                GGATG(9/13)
              333  GGATG               
HaeIII         22  GGCC                 GG^CC
               66  GGCC                
HgaI           76  GACGC                GACGC(5/10)
              120  GACGC               
HhaI           44  GCGC                 GCG^C
               82  GCGC                
HinP1I         44  GCGC                 G^CGC
               82  GCGC                
MboII         265  GAAGA                GAAGA(8/7)
              125  TCTTC               
MvaI          235  CCAGG                CC^[AT]GG
              250  CCTGG               
MvnI           43  CGCG                 CG^CG
               81  CGCG                
PleI          268  GACTC                GAGTC(4/5)
              239  GAGTC               
TaqI          150  TCGA                 T^CGA
              347  TCGA                
TseI           17  GCAGC                G^C[AT]GC
               84  GCAGC               
TthHB8I       150  TCGA                 T^CGA
              347  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        BcnI        BsaJI       BssKI       Fnu4HI      
HapII       HinfI       HpaII       ItaI        MnlI        
MspI        NciI        NlaIII      ScrFI       Tsp509I     
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