Restriction map for pF1KB5520
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CpoI        DpnII       DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MboI        
MfeI        MflI        MluI        MroI        MspA1I      
MunI        NarI        NcoI        NdeII       NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

ApaI          868  GGGCCC               GGGCC^C
AspI          408  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          663  TGGCCA               TGG^CCA
BanII         868  GGGCCC               G[GA]GC[CT]^C
BbvCI         715  GCTGAGG              CCTCAGC(-5/-2)
BcgI          699  CGACGACGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          783  GCCGCCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI          97  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI           28  GTGCAG               GTGCAG(16/14)
BspDI         567  ATCGAT               AT^CGAT
BspMI         895  GCAGGT               ACCTGC(4/8)
BsrFI         763  GCCGGC               [GA]^CCGG[CT]
Cfr10I        763  GCCGGC               [GA]^CCGG[CT]
ClaI          567  ATCGAT               AT^CGAT
DsaI          917  CCGTGG               C^C[GA][CT]GG
EarI          525  CTCTTC               CTCTTC(1/4)
EcoNI         711  CCTCGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         570  GATATC               GAT^ATC
FauI          774  CCCGC                CCCGC(4/6)
HgaI          886  GCGTC                GACGC(5/10)
HinfI         372  GAATC                G^A[ACGT]TC
HphI           43  TCACC                GGTGA(8/7)
Ksp632I       525  CTCTTC               CTCTTC(1/4)
MaeII         532  ACGT                 A^CGT
MamI           97  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         663  TGGCCA               TGG^CCA
MscI          663  TGGCCA               TGG^CCA
MslI           80  CACTGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          763  GCCGGC               GCC^GGC
NdeI          155  CATATG               CA^TATG
NgoMIV        763  GCCGGC               G^CCGGC
PflFI         408  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SmaI          865  CCCGGG               CCC^GGG
SphI          725  GCATGC               GCATG^C
TfiI          372  GAATC                G^A[AT]TC
Tth111I       408  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         865  CCCGGG               C^CCGGG
XmaI          865  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BanI          861  GGCACC               G^G[CT][GA]CC
              949  GGTGCC              
BbsI          346  GAAGAC               GAAGAC(2/6)
              295  GTCTTC              
BfaI           22  CTAG                 C^TAG
              499  CTAG                
BmyI          860  GGGCAC               G[AGT]GC[ACT]^C
              868  GGGCCC              
BpuAI         346  GAAGAC               GAAGAC(2/6)
              295  GTCTTC              
Bsp1286I      860  GGGCAC               G[AGT]GC[ACT]^C
              868  GGGCCC              
BsrI           75  ACTGG                ACTGG(1/-1)
              666  CCAGT               
BstF5I         91  CATCC                GGATG(2/0)
              171  GGATG               
BstNI         872  CCTGG                CC^[AT]GG
              981  CCTGG               
EaeI          663  TGGCCA               [CT]^GGCC[GA]
              695  TGGCCG              
EcoRII        872  CCTGG                ^CC[AT]GG
              981  CCTGG               
FokI           91  CATCC                GGATG(9/13)
              171  GGATG               
MaeI           22  CTAG                 C^TAG
              499  CTAG                
MvaI          872  CCTGG                CC^[AT]GG
              981  CCTGG               
NspI           36  ACATGC               [GA]CATG^[CT]
              725  GCATGC              
Tsp45I        495  GTCAC                ^GT[GC]AC
              503  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AcsI        ApoI        AvaI        
AvaII       BbvI        BcnI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
BssKI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EcoO109I    Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MaeIII      MboII       MnlI        MseI        MspI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
PpuMI       Sau96I      ScrFI       SfaNI       TaqI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
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