Restriction map for pF1KB5445
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaWI       
BseAI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      CelII       
CpoI        DraI        DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FbaI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MfeI        MflI        MluI        MroI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         715  GACGTC               GACGT^C
AfaI          513  GTAC                 GT^AC
AlwI          374  GGATC                GGATC(4/5)
AvaII          33  GGACC                G^G[AT]CC
BalI          257  TGGCCA               TGG^CCA
BbvCI         612  CCTCAGC              CCTCAGC(-5/-2)
BglI          562  GCCTTCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         795  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         199  GAGGAG               GAGGAG(10/8)
BsgI          484  GTGCAG               GTGCAG(16/14)
BsiWI         512  CGTACG               C^GTACG
BspDI         262  ATCGAT               AT^CGAT
BsrBI         327  CCGCTC               CCGCTC(-3/-3)
ClaI          262  ATCGAT               AT^CGAT
DraIII        619  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          402  GAAGAG               CTCTTC(1/4)
EcoRV         265  GATATC               GAT^ATC
FseI          417  GGCCGGCC             GGCCGG^CC
HinfI         438  GAATC                G^A[ACGT]TC
Ksp632I       402  GAAGAG               CTCTTC(1/4)
MamI          795  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         257  TGGCCA               TGG^CCA
MscI          257  TGGCCA               TGG^CCA
MslI          468  CATGACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          209  GCATGC               [GA]CATG^[CT]
PflMI         148  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         176  GACGCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          513  GTAC                 GT^AC
SapI          402  GAAGAGC              GCTCTTC(1/4)
SexAI         620  ACCTGGT              A^CC[AT]GGT
SmaI           29  CCCGGG               CCC^GGG
SphI          209  GCATGC               GCATG^C
StuI          122  AGGCCT               AGG^CCT
TaqI          263  TCGA                 T^CGA
TfiI          438  GAATC                G^A[AT]TC
TthHB8I       263  TCGA                 T^CGA
Van91I        148  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          386  CCATCCTCTGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          29  CCCGGG               C^CCGGG
XmaI           29  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AlwNI         187  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              783  CAGCACCTG           
AspHI         430  GTGCTC               G[AT]GC[AT]^C
              655  GTGCTC              
BanI          382  GGCACC               G^G[CT][GA]CC
              633  GGTGCC              
BpmI          745  CTCCAG               CTGGAG(16/14)
              780  CTCCAG              
BsiEI         569  CGGCCG               CG[GA][CT]^CG
              696  CGGCCG              
BsiHKAI       430  GTGCTC               G[AT]GC[AT]^C
              655  GTGCTC              
BspHI         434  TCATGA               T^CATGA
              467  TCATGA              
BspMI         150  ACCTGC               ACCTGC(4/8)
              787  ACCTGC              
BsrFI         418  GCCGGC               [GA]^CCGG[CT]
              478  GCCGGC              
BstXI         278  CCAAGCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              527  CCAGCCTCCTGG        
Cfr10I        418  GCCGGC               [GA]^CCGG[CT]
              478  GCCGGC              
DdeI           59  CTGAG                C^T[ACGT]AG
              613  CTCAG               
DpnII         375  GATC                 ^GATC
              693  GATC                
DraII          92  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              662  GGGCCCT             
EagI          569  CGGCCG               C^GGCCG
              696  CGGCCG              
EclXI         569  CGGCCG               C^GGCCG
              696  CGGCCG              
Eco52I        569  CGGCCG               C^GGCCG
              696  CGGCCG              
EcoO109I       92  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              662  GGGCCCT             
HgaI          176  GACGC                GACGC(5/10)
              310  GCGTC               
MboI          375  GATC                 ^GATC
              693  GATC                
MboII         402  GAAGA                GAAGA(8/7)
              608  TCTTC               
MspA1I        190  CAGCTG               C[AC]G^C[GT]G
              214  CAGCTG              
NaeI          418  GCCGGC               GCC^GGC
              478  GCCGGC              
NdeII         375  GATC                 ^GATC
              693  GATC                
NgoMIV        418  GCCGGC               G^CCGGC
              478  GCCGGC              
PvuII         190  CAGCTG               CAG^CTG
              214  CAGCTG              
RcaI          434  TCATGA               T^CATGA
              467  TCATGA              
Sau3AI        375  GATC                 ^GATC
              693  GATC                
TspRI         321  CAGTG                CA[GC]TG[ACGT][ACGT]^
              413  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        ApaI        
AvaI        BanII       BbvI        BcnI        BmyI        
BsaHI       BsaJI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DsaI        EaeI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NlaIII      
NlaIV       Sau96I      ScrFI       SfaNI       StyI        
TseI        Tsp45I      
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