Restriction map for pF1KB5445
 (Linear) (Six-base) MAPSORT of: KIBB5445.seq  Check: 9474  from: 1  to: 813



KIBB5445.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     159     796     813 
   Size:        159     637      17

  Fragments arranged by size: 

                 637     159      17
 
AatII G_ACGT'C

Cuts at:      0     719     813 
   Size:        719      94
 
AlwNI CAG_nnn'CTG

Cuts at:      0     192     788     813 
   Size:        192     596      25

  Fragments arranged by size: 

                 596     192      25
 
ApaI G_GGCC'C

Cuts at:      0      24      97     133     666     813 
   Size:         24      73      36     533     147

  Fragments arranged by size: 

                 533     147      73      36      24
 
AvaI C'yCGr_G

Cuts at:      0      29     111     126     813 
   Size:         29      82      15     687

  Fragments arranged by size: 

                 687      82      29      15
 
BanI G'GyrC_C

Cuts at:      0     382     633     813 
   Size:        382     251     180

  Fragments arranged by size: 

                 382     251     180
 
BanII G_rGCy'C

Cuts at:      0      24      97     104     133     526     666     813 
   Size:         24      73       7      29     393     140     147

  Fragments arranged by size: 

                 393     147     140      73      29      24       7
 
BbvCI CC'TCA_GC

Cuts at:      0     613     813 
   Size:        613     200
 
BglI GCCn_nnn'nGGC

Cuts at:      0     568     813 
   Size:        568     245
 
Bme1580I G_kGCm'C

Cuts at:      0      24      30      97     133     327     578     638     666 
   Size:         24       6      67      36     194     251      60      28

Cuts at:    666     813 
   Size:        147

  Fragments arranged by size: 

                 251     194     147      67      60      36      28      24
                   6
 
BmgBI CAC'GTC

Cuts at:      0     708     813 
   Size:        708     105
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     765     813 
   Size:        730      35      48

  Fragments arranged by size: 

                 730      48      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     613     813 
   Size:        613     200
 
BsaBI GATnn'nnATC

Cuts at:      0     799     813 
   Size:        799      14
 
BsaHI Gr'CG_yC

Cuts at:      0     177     310     716     813 
   Size:        177     133     406      97

  Fragments arranged by size: 

                 406     177     133      97
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     706     736     813 
   Size:        706      30      77

  Fragments arranged by size: 

                 706      77      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     214     813 
   Size:        214     599
 
BseYI C'CCAG_C

Cuts at:      0     285     526     813 
   Size:        285     241     287

  Fragments arranged by size: 

                 287     285     241
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     813 
   Size:        505     308
 
BsiEI CG_ry'CG

Cuts at:      0     572     699     813 
   Size:        572     127     114

  Fragments arranged by size: 

                 572     127     114
 
BsiHKAI G_wGCw'C

Cuts at:      0     434     659     813 
   Size:        434     225     154

  Fragments arranged by size: 

                 434     225     154
 
BsiWI C'GTAC_G

Cuts at:      0     512     813 
   Size:        512     301
 
Bsp1286I G_dGCh'C

Cuts at:      0      24      30      97     104     133     327     434     526 
   Size:         24       6      67       7      29     194     107      92

Cuts at:    526     578     638     659     666     813 
   Size:         52      60      21       7     147

  Fragments arranged by size: 

                 194     147     107      92      67      60      52      29
                  24      21       7       7       6
 
BspHI T'CATG_A

Cuts at:      0     434     467     813 
   Size:        434      33     346

  Fragments arranged by size: 

                 434     346      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     159     796     813 
   Size:        159     637      17

  Fragments arranged by size: 

                 637     159      17
 
BsrBI CCG'CTC

Cuts at:      0     329     813 
   Size:        329     484
 
BsrFI r'CCGG_y

Cuts at:      0     418     478     813 
   Size:        418      60     335

  Fragments arranged by size: 

                 418     335      60
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     285     534     813 
   Size:        285     249     279

  Fragments arranged by size: 

                 285     279     249
 
BtgI C'CryG_G

Cuts at:      0     332     360     548     593     813 
   Size:        332      28     188      45     220

  Fragments arranged by size: 

                 332     220     188      45      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     222     813 
   Size:        222     591
 
ClaI AT'CG_AT

Cuts at:      0     263     813 
   Size:        263     550
 
DraIII CAC_nnn'GTG

Cuts at:      0     624     813 
   Size:        624     189
 
EaeI y'GGCC_r

Cuts at:      0     257     300     416     420     476     569     696     813 
   Size:        257      43     116       4      56      93     127     117

  Fragments arranged by size: 

                 257     127     117     116      93      56      43       4
            
 
EagI C'GGCC_G

Cuts at:      0     569     696     813 
   Size:        569     127     117

  Fragments arranged by size: 

                 569     127     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     397     813 
   Size:        397     416
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     765     813 
   Size:        730      35      48

  Fragments arranged by size: 

                 730      48      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     663     813 
   Size:         93     570     150

  Fragments arranged by size: 

                 570     150      93
 
EcoRV GAT'ATC

Cuts at:      0     267     813 
   Size:        267     546
 
FseI GG_CCGG'CC

Cuts at:      0     422     813 
   Size:        422     391
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     371     403     706     738     813 
   Size:         52      32     287      32     303      32      75

  Fragments arranged by size: 

                 303     287      75      52      32      32      32
 
MscI TGG'CCA

Cuts at:      0     259     813 
   Size:        259     554
 
MslI CAynn'nnrTG

Cuts at:      0     472     813 
   Size:        472     341
 
MspA1I CmG'CkG

Cuts at:      0     192     216     813 
   Size:        192      24     597

  Fragments arranged by size: 

                 597     192      24
 
NaeI GCC'GGC

Cuts at:      0     420     480     813 
   Size:        420      60     333

  Fragments arranged by size: 

                 420     333      60
 
NcoI C'CATG_G

Cuts at:      0     332     548     593     813 
   Size:        332     216      45     220

  Fragments arranged by size: 

                 332     220     216      45
 
NgoMIV G'CCGG_C

Cuts at:      0     418     478     813 
   Size:        418      60     335

  Fragments arranged by size: 

                 418     335      60
 
NspI r_CATG'y

Cuts at:      0     213     813 
   Size:        213     600
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     154     813 
   Size:        154     659
 
PfoI T'CCnGG_A

Cuts at:      0     458     533     813 
   Size:        458      75     280

  Fragments arranged by size: 

                 458     280      75
 
PshAI GACnn'nnGTC

Cuts at:      0     180     813 
   Size:        180     633
 
PspOMI G'GGCC_C

Cuts at:      0      20      93     129     662     813 
   Size:         20      73      36     533     151

  Fragments arranged by size: 

                 533     151      73      36      20
 
PvuII CAG'CTG

Cuts at:      0     192     216     813 
   Size:        192      24     597

  Fragments arranged by size: 

                 597     192      24
 
SapI GCTCTTCn'nnn_

Cuts at:      0     397     813 
   Size:        397     416
 
SexAI A'CCwGG_T

Cuts at:      0     620     813 
   Size:        620     193
 
SmaI CCC'GGG

Cuts at:      0      31     813 
   Size:         31     782
 
SphI G_CATG'C

Cuts at:      0     213     813 
   Size:        213     600
 
StuI AGG'CCT

Cuts at:      0     124     813 
   Size:        124     689
 
StyI C'CwwG_G

Cuts at:      0      86     332     548     593     813 
   Size:         86     246     216      45     220

  Fragments arranged by size: 

                 246     220     216      86      45
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     393     813 
   Size:        393     420
 
XmaI C'CCGG_G

Cuts at:      0      29     813 
   Size:         29     784
 
ZraI GAC'GTC

Cuts at:      0     717     813 
   Size:        717      96
 

 Enzymes that do cut:

     AarI    AatII    AlwNI     ApaI     AvaI     BanI    BanII    BbvCI
     BglI Bme1580I    BmgBI     BpmI   Bpu10I    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BstXI     BtgI    BtgZI     ClaI   DraIII
     EaeI     EagI     EarI  Eco57MI EcoO109I    EcoRV     FseI    Hin4I
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI    PflMI
     PfoI    PshAI   PspOMI    PvuII     SapI    SexAI     SmaI     SphI
     StuI     StyI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtsI    CspCI     DraI     DrdI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
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