Restriction map for pF1KB5418
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsoBI       
Bsp1407I    BspEI       BspHI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       FbaI        FseI        FspI        HaeIII      
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NarI        NciI        NcoI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SbfI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AflIII        558  ACATGT               A^C[GA][CT]GT
Aor51HI       125  AGCGCT               AGC^GCT
Asp700I      1174  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1037  GAGCAC               G[AT]GC[AT]^C
AvrII        1059  CCTAGG               C^CTAGG
BbsI          309  GTCTTC               GAAGAC(2/6)
BfaI         1060  CTAG                 C^TAG
BlnI         1059  CCTAGG               C^CTAGG
BpmI         1086  CTGGAG               CTGGAG(16/14)
BpuAI         309  GTCTTC               GAAGAC(2/6)
BsaI          272  GAGACC               GGTCTC(1/5)
BsgI          889  GTGCAG               GTGCAG(16/14)
BsiEI        1242  CGATCG               CG[GA][CT]^CG
BsiHKAI      1037  GAGCAC               G[AT]GC[AT]^C
BsmI         1100  GCATTC               GAATGC(1/-1)
BspLU11I      558  ACATGT               A^CATGT
BspMI         176  GCAGGT               ACCTGC(4/8)
BsrDI        1165  GCAATG               GCAATG(2/0)
BstAPI       1091  GCATAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        439  GGTCACC              G^GT[ACGT]ACC
BstPI         439  GGTCACC              G^GT[ACGT]ACC
CfoI          126  GCGC                 GCG^C
Eco47III      125  AGCGCT               AGC^GCT
EcoO65I       439  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1059  CCTAGG               C^C[AT][AT]GG
EcoT22I      1098  ATGCAT               ATGCA^T
FauI          547  GCGGG                CCCGC(4/6)
HaeII         125  AGCGCT               [GA]GCGC^[CT]
HhaI          126  GCGC                 GCG^C
HinP1I        126  GCGC                 G^CGC
MaeI         1060  CTAG                 C^TAG
MslI          103  CATAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        666  CCGCTG               C[AC]G^C[GT]G
MwoI         1091  GCATAGGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI         1273  GCCGGC               GCC^GGC
NgoMIV       1273  GCCGGC               G^CCGGC
NlaIV        1067  GGTCCC               GG[ACGT]^[ACGT]CC
NsiI         1098  ATGCAT               ATGCA^T
NspI          558  ACATGT               [GA]CATG^[CT]
PvuI         1242  CGATCG               CGAT^CG
SalI          206  GTCGAC               G^TCGAC
SspI         1229  AATATT               AAT^ATT
StyI         1059  CCTAGG               C^C[AT][AT]GG
TspRI         108  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI         1075  CCATGTGATGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1174  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AciI          666  CCGC                 CCGC(-3/-1)
              547  GCGG                
AcsI          828  GAATTT               [GA]^AATT[CT]
             1312  GAATTT              
AluI          510  AGCT                 AG^CT
              603  AGCT                
AlwI         1381  GATCC                GGATC(4/5)
              786  GGATC               
ApoI          828  GAATTT               [GA]^AATT[CT]
             1312  GAATTT              
AvaII        1067  GGTCC                G^G[AT]CC
             1186  GGACC               
BmyI          110  GTGCCC               G[AGT]GC[ACT]^C
             1037  GAGCAC              
BsaJI         113  CCCAGG               C^C[ACGT][ACGT]GG
             1059  CCTAGG              
BseRI          66  GAGGAG               GAGGAG(10/8)
              433  GAGGAG              
BsmBI        1345  CGTCTC               CGTCTC(1/5)
              461  GAGACG              
BsmFI         732  GGGAC                GGGAC(10/14)
             1068  GTCCC               
Bsp1286I      110  GTGCCC               G[AGT]GC[ACT]^C
             1037  GAGCAC              
BspDI         535  ATCGAT               AT^CGAT
             1244  ATCGAT              
BsrFI         284  GCCGGT               [GA]^CCGG[CT]
             1273  GCCGGC              
BsrI          783  ACTGG                ACTGG(1/-1)
             1253  ACTGG               
Cac8I         607  GCTCGC               GC[ACGT]^[ACGT]GC
             1273  GCCGGC              
Cfr10I        284  GCCGGT               [GA]^CCGG[CT]
             1273  GCCGGC              
Cfr13I       1067  GGTCC                G^G[ACGT]CC
             1186  GGACC               
ClaI          535  ATCGAT               AT^CGAT
             1244  ATCGAT              
EarI           93  GAAGAG               CTCTTC(1/4)
              390  GAAGAG              
HapII         285  CCGG                 C^CGG
             1274  CCGG                
HincII        206  GTCGAC               GT[CT]^[GA]AC
              849  GTTGAC              
HindII        206  GTCGAC               GT[CT]^[GA]AC
              849  GTTGAC              
HpaII         285  CCGG                 C^CGG
             1274  CCGG                
Ksp632I        93  GAAGAG               CTCTTC(1/4)
              390  GAAGAG              
MspI          285  CCGG                 C^CGG
             1274  CCGG                
NdeI          989  CATATG               CA^TATG
             1201  CATATG              
PflMI         162  CCATTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              675  CCAGATGTTGG         
PleI          852  GACTC                GAGTC(4/5)
              393  GAGTC               
Sau96I       1067  GGTCC                G^G[ACGT]CC
             1186  GGACC               
SmlI          242  CTCAAG               C^T[CT][GA]AG
             1325  CTCAAG              
TfiI          354  GATTC                G^A[AT]TC
             1179  GATTC               
Van91I        162  CCATTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              675  CCAGATGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccI        AfaI        BbvI        BsiYI       BslI        
BsmAI       BssKI       BstF5I      BstNI       DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HgaI        
HinfI       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
NdeII       NlaIII      RsaI        Sau3AI      ScaI        
ScrFI       SfaNI       TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TthHB8I     
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