Restriction map for pF1KB5418
 (Linear) (Six-base) MAPSORT of: KIBB5418.seq  Check: 7863  from: 1  to: 1452



KIBB5418.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AccI GT'mk_AC

Cuts at:      0     207     555     971    1452 
   Size:        207     348     416     481

  Fragments arranged by size: 

                 481     416     348     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    1327    1452 
   Size:       1064     263     125

  Fragments arranged by size: 

                1064     263     125
 
AfeI AGC'GCT

Cuts at:      0     127    1452 
   Size:        127    1325
 
AflIII A'CryG_T

Cuts at:      0     558    1452 
   Size:        558     894
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       9      41    1452 
   Size:          9      32    1411

  Fragments arranged by size: 

                1411      32       9
 
ApoI r'AATT_y

Cuts at:      0     828    1312    1452 
   Size:        828     484     140

  Fragments arranged by size: 

                 828     484     140
 
AvrII C'CTAG_G

Cuts at:      0    1059    1452 
   Size:       1059     393
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1001    1034    1452 
   Size:       1001      33     418

  Fragments arranged by size: 

                1001     418      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     302    1452 
   Size:        302    1150
 
BfrBI ATG'CAT

Cuts at:      0    1100    1452 
   Size:       1100     352
 
Bme1580I G_kGCm'C

Cuts at:      0     114    1452 
   Size:        114    1338
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1263    1452 
   Size:       1263     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1107    1452 
   Size:       1107     345
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227    1310    1452 
   Size:        227    1083     142

  Fragments arranged by size: 

                1083     227     142
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     266    1452 
   Size:        266    1186
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      81     448    1452 
   Size:         81     367    1004

  Fragments arranged by size: 

                1004     367      81
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910    1452 
   Size:        910     542
 
BsiEI CG_ry'CG

Cuts at:      0    1245    1452 
   Size:       1245     207
 
BsiHKAI G_wGCw'C

Cuts at:      0    1041    1452 
   Size:       1041     411
 
BsmI GAATG_Cn'

Cuts at:      0    1100    1452 
   Size:       1100     352
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     455    1351    1452 
   Size:        455     896     101

  Fragments arranged by size: 

                 896     455     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     114    1041    1452 
   Size:        114     927     411

  Fragments arranged by size: 

                 927     411     114
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167    1452 
   Size:        167    1285
 
BsrDI GCAATG_nn'

Cuts at:      0    1172    1452 
   Size:       1172     280
 
BsrFI r'CCGG_y

Cuts at:      0     284    1273    1452 
   Size:        284     989     179

  Fragments arranged by size: 

                 989     284     179
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1097    1452 
   Size:       1097     355
 
BstEII G'GTnAC_C

Cuts at:      0     439    1452 
   Size:        439    1013
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     247    1452 
   Size:        247    1205
 
BtsI GCAGTG_nn'

Cuts at:      0     114    1452 
   Size:        114    1338
 
ClaI AT'CG_AT

Cuts at:      0     536    1245    1452 
   Size:        536     709     207

  Fragments arranged by size: 

                 709     536     207
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     385    1452 
   Size:         88     297    1067

  Fragments arranged by size: 

                1067     297      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    1107    1327    1452 
   Size:       1064      43     220     125

  Fragments arranged by size: 

                1064     220     125      43
 
HaeII r_GCGC'y

Cuts at:      0     129    1452 
   Size:        129    1323
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     792     824    1452 
   Size:        792      32     628

  Fragments arranged by size: 

                 792     628      32
 
HincII GTy'rAC

Cuts at:      0     208     851    1452 
   Size:        208     643     601

  Fragments arranged by size: 

                 643     601     208
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     662    1162    1352    1452 
   Size:        662     500     190     100

  Fragments arranged by size: 

                 662     500     190     100
 
MslI CAynn'nnrTG

Cuts at:      0     107    1452 
   Size:        107    1345
 
MspA1I CmG'CkG

Cuts at:      0     668    1452 
   Size:        668     784
 
NaeI GCC'GGC

Cuts at:      0    1275    1452 
   Size:       1275     177
 
NdeI CA'TA_TG

Cuts at:      0     990    1202    1452 
   Size:        990     212     250

  Fragments arranged by size: 

                 990     250     212
 
NgoMIV G'CCGG_C

Cuts at:      0    1273    1452 
   Size:       1273     179
 
NsiI A_TGCA'T

Cuts at:      0    1102    1452 
   Size:       1102     350
 
NspI r_CATG'y

Cuts at:      0     562    1452 
   Size:        562     890
 
PciI A'CATG_T

Cuts at:      0     558    1452 
   Size:        558     894
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     168     681    1452 
   Size:        168     513     771

  Fragments arranged by size: 

                 771     513     168
 
PsiI TTA'TAA

Cuts at:      0     338    1452 
   Size:        338    1114
 
PvuI CG_AT'CG

Cuts at:      0    1245    1452 
   Size:       1245     207
 
SalI G'TCGA_C

Cuts at:      0     206    1452 
   Size:        206    1246
 
ScaI AGT'ACT

Cuts at:      0     366     660     741    1452 
   Size:        366     294      81     711

  Fragments arranged by size: 

                 711     366     294      81
 
SmlI C'TyrA_G

Cuts at:      0     242    1325    1452 
   Size:        242    1083     127

  Fragments arranged by size: 

                1083     242     127
 
SspI AAT'ATT

Cuts at:      0    1231    1452 
   Size:       1231     221
 
StyI C'CwwG_G

Cuts at:      0    1059    1452 
   Size:       1059     393
 
TatI w'GTAC_w

Cuts at:      0     224     364     658     739    1009    1452 
   Size:        224     140     294      81     270     443

  Fragments arranged by size: 

                 443     294     270     224     140      81
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     509     541    1452 
   Size:        509      32     911

  Fragments arranged by size: 

                 911     509      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1082    1452 
   Size:       1082     370
 
XmnI GAAnn'nnTTC

Cuts at:      0    1178    1452 
   Size:       1178     274
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AloI     ApoI    AvrII     BaeI
     BbsI    BfrBI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BseRI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrFI   BstAPI   BstEII    BtgZI     BtsI     ClaI     EarI  Eco57MI
    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I     NaeI     NdeI
   NgoMIV     NsiI     NspI     PciI    PflMI     PsiI     PvuI     SalI
     ScaI     SmlI     SspI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiWI    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NarI     NcoI     NheI     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     ZraI
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