Restriction map for pF1KB5300
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       BamHI       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BglII       BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CfoI        ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MslI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TfiI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI           67  GTCGAC               GT^[AC][GT]AC
AcsI          142  AAATTC               [GA]^AATT[CT]
AfaI          225  GTAC                 GT^AC
AflII         733  CTTAAG               C^TTAAG
AhdI          802  GACCTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          660  GATCC                GGATC(4/5)
ApoI          142  AAATTC               [GA]^AATT[CT]
AspEI         802  GACCTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         189  CCTAGG               C^CTAGG
BanI         1189  GGTGCC               G^G[CT][GA]CC
BbvCI         973  GCTGAGG              CCTCAGC(-5/-2)
BfrI          733  CTTAAG               C^TTAAG
BlnI          189  CCTAGG               C^CTAGG
BlpI          991  GCTAAGC              GC^T[ACGT]AGC
BpmI          355  CTGGAG               CTGGAG(16/14)
Bpu1102I      991  GCTAAGC              GC^T[ACGT]AGC
BsmAI        1121  GAGAC                GTCTC(1/5)
BsmFI         130  GTCCC                GGGAC(10/14)
BsmI          882  GAATGC               GAATGC(1/-1)
BspMI         921  ACCTGC               ACCTGC(4/8)
BsrFI         194  GCCGGC               [GA]^CCGG[CT]
BstYI         659  AGATCC               [GA]^GATC[CT]
CelII         991  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        194  GCCGGC               [GA]^CCGG[CT]
DpnII         660  GATC                 ^GATC
DsaI          524  CCATGG               C^C[GA][CT]GG
Eam1105I      802  GACCTAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          778  GAAGAG               CTCTTC(1/4)
FauI           51  GCGGG                CCCGC(4/6)
FseI          193  GGCCGGCC             GGCCGG^CC
HgaI           21  GACGC                GACGC(5/10)
Ksp632I       778  GAAGAG               CTCTTC(1/4)
MaeII          38  ACGT                 A^CGT
MboI          660  GATC                 ^GATC
MflI          659  AGATCC               [GA]^GATC[CT]
MspA1I        332  CAGCTG               C[AC]G^C[GT]G
NaeI          194  GCCGGC               GCC^GGC
NcoI          524  CCATGG               C^CATGG
NdeII         660  GATC                 ^GATC
NgoMIV        194  GCCGGC               G^CCGGC
PflMI         331  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         943  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         332  CAGCTG               CAG^CTG
RsaI          225  GTAC                 GT^AC
SacI         1201  GAGCTC               GAGCT^C
SalI           67  GTCGAC               G^TCGAC
Sau3AI        660  GATC                 ^GATC
ScaI          224  AGTACT               AGT^ACT
SfcI          245  CTGTAG               C^T[GA][CT]AG
SmaI         1046  CCCGGG               CCC^GGG
SmlI          733  CTTAAG               C^T[CT][GA]AG
SspI         1246  AATATT               AAT^ATT
StuI          596  AGGCCT               AGG^CCT
TaqI           68  TCGA                 T^CGA
Tsp509I       143  AATT                 ^AATT
TthHB8I        68  TCGA                 T^CGA
Van91I        331  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         659  AGATCC               [GA]^GATC[CT]
XmaCI        1046  CCCGGG               C^CCGGG
XmaI         1046  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI         239  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              332  CAGCTGCTG           
AspI          685  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              853  GACTTTGTC           
AvaI          986  CTCGGG               C^[CT]CG[GA]G
             1046  CCCGGG              
AvaII         949  GGTCC                G^G[AT]CC
             1012  GGTCC               
BalI          626  TGGCCA               TGG^CCA
              894  TGGCCA              
BcnI         1046  CCCGG                CC^[GC]GG
             1047  CCGGG               
BglI          185  GCCACCTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              901  GCCACTGTGGC         
BsoBI         986  CTCGGG               C^[CT]CG[GA]G
             1046  CCCGGG              
EaeI          626  TGGCCA               [CT]^GGCC[GA]
              894  TGGCCA              
HapII         195  CCGG                 C^CGG
             1047  CCGG                
HincII         67  GTCGAC               GT[CT]^[GA]AC
              850  GTTGAC              
HindII         67  GTCGAC               GT[CT]^[GA]AC
              850  GTTGAC              
HindIII       443  AAGCTT               A^AGCTT
             1037  AAGCTT              
HinfI          65  GAGTC                G^A[ACGT]TC
              533  GAGTC               
HpaII         195  CCGG                 C^CGG
             1047  CCGG                
HphI         1077  GGTGA                GGTGA(8/7)
             1103  TCACC               
MaeIII        859  GTCAC                ^GT[ACGT]AC
              982  GTCAC               
MluNI         626  TGGCCA               TGG^CCA
              894  TGGCCA              
MscI          626  TGGCCA               TGG^CCA
              894  TGGCCA              
MspI          195  CCGG                 C^CGG
             1047  CCGG                
NciI         1046  CCCGG                CC^[GC]GG
             1047  CCGGG               
PflFI         685  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              853  GACTTTGTC           
PleI           65  GAGTC                GAGTC(4/5)
              533  GAGTC               
Tsp45I        859  GTCAC                ^GT[GC]AC
              982  GTCAC               
Tth111I       685  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              853  GACTTTGTC           
XcmI         1055  CCAAAGCTCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1170  CCATGTGAGCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        AspHI       BanII       BbvI        
BfaI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      ItaI        
MaeI        MboII       MnlI        MseI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       StyI        Tru9I       TseI        TspRI       
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