Restriction map for
pF1KB5300
(Linear) (Six-base) MAPSORT of: KIBB5300.seq Check: 3685 from: 1 to: 1251
KIBB5300.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 68 1251
Size: 68 1183
AflII C'TTAA_G
Cuts at: 0 733 1251
Size: 733 518
AhdI GACnn_n'nnGTC
Cuts at: 0 807 1251
Size: 807 444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1251
Size: 265 32 954
Fragments arranged by size:
954 265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 244 337 1251
Size: 244 93 914
Fragments arranged by size:
914 244 93
ApoI r'AATT_y
Cuts at: 0 142 1251
Size: 142 1109
AvaI C'yCGr_G
Cuts at: 0 986 1046 1251
Size: 986 60 205
Fragments arranged by size:
986 205 60
AvrII C'CTAG_G
Cuts at: 0 189 1251
Size: 189 1062
BanI G'GyrC_C
Cuts at: 0 1189 1251
Size: 1189 62
BanII G_rGCy'C
Cuts at: 0 516 610 1205 1251
Size: 516 94 595 46
Fragments arranged by size:
595 516 94 46
BbvCI CC'TCA_GC
Cuts at: 0 974 1251
Size: 974 277
BglI GCCn_nnn'nGGC
Cuts at: 0 191 907 1251
Size: 191 716 344
Fragments arranged by size:
716 344 191
BlpI GC'TnA_GC
Cuts at: 0 992 1251
Size: 992 259
Bme1580I G_kGCm'C
Cuts at: 0 212 1251
Size: 212 1039
BmrI ACTGGGnnnn_n'
Cuts at: 0 1190 1251
Size: 1190 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1251
Size: 376 875
Bpu10I CC'TnA_GC
Cuts at: 0 974 1251
Size: 974 277
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 211 241 632 662 1251
Size: 211 30 391 30 589
Fragments arranged by size:
589 391 211 30 30
BseYI C'CCAG_C
Cuts at: 0 264 330 337 1251
Size: 264 66 7 914
Fragments arranged by size:
914 264 66 7
BsiHKAI G_wGCw'C
Cuts at: 0 743 1180 1205 1251
Size: 743 437 25 46
Fragments arranged by size:
743 437 46 25
BsmI GAATG_Cn'
Cuts at: 0 888 1251
Size: 888 363
Bsp1286I G_dGCh'C
Cuts at: 0 212 516 610 743 1180 1205 1251
Size: 212 304 94 133 437 25 46
Fragments arranged by size:
437 304 212 133 94 46 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 930 1251
Size: 930 321
BsrFI r'CCGG_y
Cuts at: 0 194 1251
Size: 194 1057
BstYI r'GATC_y
Cuts at: 0 659 1251
Size: 659 592
BtgI C'CryG_G
Cuts at: 0 524 1251
Size: 524 727
BtsI GCAGTG_nn'
Cuts at: 0 500 860 1124 1251
Size: 500 360 264 127
Fragments arranged by size:
500 360 264 127
EaeI y'GGCC_r
Cuts at: 0 626 894 1251
Size: 626 268 357
Fragments arranged by size:
626 357 268
EarI CTCTTCn'nnn_
Cuts at: 0 773 1251
Size: 773 478
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 653 1251
Size: 653 598
EcoICRI GAG'CTC
Cuts at: 0 1203 1251
Size: 1203 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1251
Size: 376 875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 246 858 1251
Size: 246 612 393
Fragments arranged by size:
612 393 246
FseI GG_CCGG'CC
Cuts at: 0 198 1251
Size: 198 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 793 825 1251
Size: 211 32 550 32 426
Fragments arranged by size:
550 426 211 32 32
HincII GTy'rAC
Cuts at: 0 69 852 1251
Size: 69 783 399
Fragments arranged by size:
783 399 69
HindIII A'AGCT_T
Cuts at: 0 443 1037 1251
Size: 443 594 214
Fragments arranged by size:
594 443 214
MscI TGG'CCA
Cuts at: 0 628 896 1251
Size: 628 268 355
Fragments arranged by size:
628 355 268
MspA1I CmG'CkG
Cuts at: 0 334 1251
Size: 334 917
NaeI GCC'GGC
Cuts at: 0 196 1251
Size: 196 1055
NcoI C'CATG_G
Cuts at: 0 524 1251
Size: 524 727
NgoMIV G'CCGG_C
Cuts at: 0 194 1251
Size: 194 1057
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 1251
Size: 337 914
PfoI T'CCnGG_A
Cuts at: 0 1205 1251
Size: 1205 46
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1044 1076 1251
Size: 1044 32 175
Fragments arranged by size:
1044 175 32
PshAI GACnn'nnGTC
Cuts at: 0 947 1251
Size: 947 304
PvuII CAG'CTG
Cuts at: 0 334 1251
Size: 334 917
SacI G_AGCT'C
Cuts at: 0 1205 1251
Size: 1205 46
SalI G'TCGA_C
Cuts at: 0 67 1251
Size: 67 1184
ScaI AGT'ACT
Cuts at: 0 226 1251
Size: 226 1025
SfcI C'TryA_G
Cuts at: 0 245 1251
Size: 245 1006
SmaI CCC'GGG
Cuts at: 0 1048 1251
Size: 1048 203
SmlI C'TyrA_G
Cuts at: 0 733 1251
Size: 733 518
SspI AAT'ATT
Cuts at: 0 1248 1251
Size: 1248 3
StuI AGG'CCT
Cuts at: 0 598 1251
Size: 598 653
StyI C'CwwG_G
Cuts at: 0 189 524 599 1251
Size: 189 335 75 652
Fragments arranged by size:
652 335 189 75
TatI w'GTAC_w
Cuts at: 0 224 1251
Size: 224 1027
Tth111I GACn'n_nGTC
Cuts at: 0 688 856 1251
Size: 688 168 395
Fragments arranged by size:
688 395 168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1177 1251
Size: 1062 115 74
Fragments arranged by size:
1062 115 74
XmaI C'CCGG_G
Cuts at: 0 1046 1251
Size: 1046 205
Enzymes that do cut:
AccI AflII AhdI AloI AlwNI ApoI AvaI AvrII
BanI BanII BbvCI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaXI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
FalI FseI Hin4I HincII HindIII MscI MspA1I NaeI
NcoI NgoMIV PflMI PfoI PpiI PshAI PvuII SacI
SalI ScaI SfcI SmaI SmlI SspI StuI StyI
TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoNI EcoO109I EcoRI EcoRV FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MmeI MslI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TaqII TstI XbaI XhoI XmnI ZraI