Restriction map for pF1KB5285
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspHI       
AviII       AvrII       BamHI       BbeI        BcgI        
BciVI       BfrI        BlnI        BsaBI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HindIII     HpaI        KasI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       1258  GGTACC               G^GTACC
AcyI           80  GACGCC               G[GA]^CG[CT]C
AocI          924  CCTGAGG              CC^T[ACGT]AGG
Asp718I      1258  GGTACC               G^GTACC
AspI         1327  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2152  CCCGAG               C^[CT]CG[GA]G
BalI         1301  TGGCCA               TGG^CCA
BanI         1258  GGTACC               G^G[CT][GA]CC
BanII        2083  GAGCCC               G[GA]GC[CT]^C
BbrPI         819  CACGTG               CAC^GTG
BclI         1529  TGATCA               T^GATCA
BglI         1736  GCCCAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1851  AGATCT               A^GATCT
BlpI         2080  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2080  GCTGAGC              GC^T[ACGT]AGC
BsaAI         819  CACGTG               [CT]AC^GT[GA]
BsaHI          80  GACGCC               G[GA]^CG[CT]C
BsmBI          78  GAGACG               CGTCTC(1/5)
BsoBI        2152  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      171  TGTACA               T^GTACA
BspHI        1428  TCATGA               T^CATGA
BsrGI         171  TGTACA               T^GTACA
BssSI        1039  CACGAG               CACGAG(-5/-1)
Bsu36I        924  CCTGAGG              CC^T[ACGT]AGG
CelII        2080  GCTGAGC              GC^T[ACGT]AGC
DraII         434  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI         1301  TGGCCA               [CT]^GGCC[GA]
Eco81I        924  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      434  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      1706  CCATGG               C^C[AT][AT]GG
FbaI         1529  TGATCA               T^GATCA
Hin1I          80  GACGCC               G[GA]^CG[CT]C
HincII        160  GTCAAC               GT[CT]^[GA]AC
HindII        160  GTCAAC               GT[CT]^[GA]AC
KpnI         1258  GGTACC               GGTAC^C
MfeI         2039  CAATTG               C^AATTG
MluNI        1301  TGGCCA               TGG^CCA
MscI         1301  TGGCCA               TGG^CCA
MunI         2039  CAATTG               C^AATTG
NcoI         1706  CCATGG               C^CATGG
PflFI        1327  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           34  GAGTC                GAGTC(4/5)
PmaCI         819  CACGTG               CAC^GTG
PmlI          819  CACGTG               CAC^GTG
PstI         1572  CTGCAG               CTGCA^G
RcaI         1428  TCATGA               T^CATGA
SfcI         1572  CTGCAG               C^T[GA][CT]AG
SspBI         171  TGTACA               T^GTACA
StyI         1706  CCATGG               C^C[AT][AT]GG
Tth111I      1327  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          673  GTCTAC               GT^[AC][GT]AC
             1511  GTAGAC              
AcsI          837  AAATTT               [GA]^AATT[CT]
             1306  AAATTT              
ApoI          837  AAATTT               [GA]^AATT[CT]
             1306  AAATTT              
BbvCI        1912  CCTCAGC              CCTCAGC(-5/-2)
             2163  CCTCAGC             
BcnI           84  CCGGG                CC^[GC]GG
             1553  CCGGG               
BfaI          532  CTAG                 C^TAG
              566  CTAG                
BpmI          248  CTCCAG               CTGGAG(16/14)
             1121  CTGGAG              
BsaI          873  GAGACC               GGTCTC(1/5)
             2141  GAGACC              
BsgI         1140  GTGCAG               GTGCAG(16/14)
             1149  GTGCAG              
BsmI          904  GCATTC               GAATGC(1/-1)
             1377  GCATTC              
BstXI         129  CCAGACGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              888  CCATCTCTGTGG        
BstYI         561  AGATCC               [GA]^GATC[CT]
             1851  AGATCT              
DsaI          826  CCACGG               C^C[GA][CT]GG
             1706  CCATGG              
EarI         1776  GAAGAG               CTCTTC(1/4)
             2101  GAAGAG              
FauI         1939  CCCGC                CCCGC(4/6)
             2086  CCCGC               
HgaI           80  GACGC                GACGC(5/10)
             1128  GCGTC               
Ksp632I      1776  GAAGAG               CTCTTC(1/4)
             2101  GAAGAG              
MaeI          532  CTAG                 C^TAG
              566  CTAG                
MflI          561  AGATCC               [GA]^GATC[CT]
             1851  AGATCT              
MspA1I        193  CAGCTG               C[AC]G^C[GT]G
             2117  CAGCTG              
NciI           84  CCGGG                CC^[GC]GG
             1553  CCGGG               
NspI         1616  ACATGC               [GA]CATG^[CT]
             1716  ACATGC              
PvuII         193  CAGCTG               CAG^CTG
             2117  CAGCTG              
TaqI           17  TCGA                 T^CGA
             1613  TCGA                
TfiI          930  GAATC                G^A[AT]TC
             1822  GATTC               
TthHB8I        17  TCGA                 T^CGA
             1613  TCGA                
XcmI          512  CCAATGGAGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              998  CCAGTGGAGAATTGG     
XhoII         561  AGATCC               [GA]^GATC[CT]
             1851  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AlwNI       Aor51HI     Asp700I     AvaII       BbsI        
BbvI        BmyI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
Eco47III    EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XmnI        
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