Restriction map for pF1KB5285
 (Linear) (Six-base) MAPSORT of: KIBB5285.seq  Check: 5000  from: 1  to: 2181



KIBB5285.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0     674    1512    2181 
   Size:        674     838     669

  Fragments arranged by size: 

                 838     674     669
 
Acc65I G'GTAC_C

Cuts at:      0    1258    2181 
   Size:       1258     923
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     245     322     371     385     503    2181 
   Size:        196      49      77      49      14     118    1678

  Fragments arranged by size: 

                1678     196     118      77      49      49      14
 
AfeI AGC'GCT

Cuts at:      0    1435    1480    1630    2181 
   Size:       1435      45     150     551

  Fragments arranged by size: 

                1435     551     150      45
 
AflIII A'CryG_T

Cuts at:      0     818     820    1693    2181 
   Size:        818       2     873     488

  Fragments arranged by size: 

                 873     818     488       2
 
AleI CACnn'nnGTG

Cuts at:      0     882    2181 
   Size:        882    1299
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     995    1027    2181 
   Size:        995      32    1154

  Fragments arranged by size: 

                1154     995      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     262    1580    2080    2181 
   Size:        262    1318     500     101

  Fragments arranged by size: 

                1318     500     262     101
 
ApoI r'AATT_y

Cuts at:      0     837    1306    2181 
   Size:        837     469     875

  Fragments arranged by size: 

                 875     837     469
 
AvaI C'yCGr_G

Cuts at:      0    2152    2181 
   Size:       2152      29
 
BanI G'GyrC_C

Cuts at:      0    1258    2181 
   Size:       1258     923
 
BanII G_rGCy'C

Cuts at:      0    2087    2181 
   Size:       2087      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     226     796    2027    2181 
   Size:        226     570    1231     154

  Fragments arranged by size: 

                1231     570     226     154
 
BbvCI CC'TCA_GC

Cuts at:      0    1913    2164    2181 
   Size:       1913     251      17

  Fragments arranged by size: 

                1913     251      17
 
BclI T'GATC_A

Cuts at:      0    1529    2181 
   Size:       1529     652
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1742    2181 
   Size:       1742     439
 
BglII A'GATC_T

Cuts at:      0    1851    2181 
   Size:       1851     330
 
BlpI GC'TnA_GC

Cuts at:      0    2081    2181 
   Size:       2081     100
 
Bme1580I G_kGCm'C

Cuts at:      0     241     973    2181 
   Size:        241     732    1208

  Fragments arranged by size: 

                1208     732     241
 
BmgBI CAC'GTC

Cuts at:      0    1696    2181 
   Size:       1696     485
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233    1142    2181 
   Size:        233     909    1039

  Fragments arranged by size: 

                1039     909     233
 
Bpu10I CC'TnA_GC

Cuts at:      0    1901    1913    2164    2181 
   Size:       1901      12     251      17

  Fragments arranged by size: 

                1901     251      17      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     867    2135    2181 
   Size:        867    1268      46

  Fragments arranged by size: 

                1268     867      46
 
BsaAI yAC'GTr

Cuts at:      0     821    2181 
   Size:        821    1360
 
BsaHI Gr'CG_yC

Cuts at:      0      81    2181 
   Size:         81    2100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     231     261     995    1025    2181 
   Size:        231      30     734      30    1156

  Fragments arranged by size: 

                1156     734     231      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     661    1778    1888    1891    2119    2181 
   Size:         46     615    1117     110       3     228      62

  Fragments arranged by size: 

                1117     615     228     110      62      46       3
 
BseYI C'CCAG_C

Cuts at:      0    1926    2111    2181 
   Size:       1926     185      70

  Fragments arranged by size: 

                1926     185      70
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1161    1170    2181 
   Size:       1161       9    1011

  Fragments arranged by size: 

                1161    1011       9
 
BsmI GAATG_Cn'

Cuts at:      0     904    1377    2181 
   Size:        904     473     804

  Fragments arranged by size: 

                 904     804     473
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      72    2181 
   Size:         72    2109
 
Bsp1286I G_dGCh'C

Cuts at:      0     241     973    2087    2181 
   Size:        241     732    1114      94

  Fragments arranged by size: 

                1114     732     241      94
 
BspHI T'CATG_A

Cuts at:      0    1428    2181 
   Size:       1428     753
 
BsrGI T'GTAC_A

Cuts at:      0     171    2181 
   Size:        171    2010
 
BssSI C'ACGA_G

Cuts at:      0    1039    2181 
   Size:       1039    1142
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     262    1148    1403    1580    1717    2181 
   Size:        262     886     255     177     137     464

  Fragments arranged by size: 

                 886     464     262     255     177     137
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     136     895    2181 
   Size:        136     759    1286

  Fragments arranged by size: 

                1286     759     136
 
BstYI r'GATC_y

Cuts at:      0     561    1851    2181 
   Size:        561    1290     330

  Fragments arranged by size: 

                1290     561     330
 
Bsu36I CC'TnA_GG

Cuts at:      0     925    2181 
   Size:        925    1256
 
BtgI C'CryG_G

Cuts at:      0     826    1706    2181 
   Size:        826     880     475

  Fragments arranged by size: 

                 880     826     475
 
BtsI GCAGTG_nn'

Cuts at:      0     174    1578    2181 
   Size:        174    1404     603

  Fragments arranged by size: 

                1404     603     174
 
EaeI y'GGCC_r

Cuts at:      0    1301    2181 
   Size:       1301     880
 
EarI CTCTTCn'nnn_

Cuts at:      0    1771    2096    2181 
   Size:       1771     325      85

  Fragments arranged by size: 

                1771     325      85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1585    2181 
   Size:       1585     596
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     233     245     322     371     385     503    1142 
   Size:        196      37      12      77      49      14     118     639

Cuts at:   1142    2181 
   Size:       1039

  Fragments arranged by size: 

                1039     639     196     118      77      49      37      14
                  12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     435    2181 
   Size:        435    1746
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     454     562    2181 
   Size:        454     108    1619

  Fragments arranged by size: 

                1619     454     108
 
HaeII r_GCGC'y

Cuts at:      0    1437    1482    1632    2181 
   Size:       1437      45     150     549

  Fragments arranged by size: 

                1437     549     150      45
 
HincII GTy'rAC

Cuts at:      0     162    2181 
   Size:        162    2019
 
KpnI G_GTAC'C

Cuts at:      0    1262    2181 
   Size:       1262     919
 
MfeI C'AATT_G

Cuts at:      0    2039    2181 
   Size:       2039     142
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1678    2181 
   Size:       1678     503
 
MscI TGG'CCA

Cuts at:      0    1303    2181 
   Size:       1303     878
 
MslI CAynn'nnrTG

Cuts at:      0     798     866     882     893    1053    1267    1469    1531 
   Size:        798      68      16      11     160     214     202      62

Cuts at:   1531    2181 
   Size:        650

  Fragments arranged by size: 

                 798     650     214     202     160      68      62      16
                  11
 
MspA1I CmG'CkG

Cuts at:      0     195    2119    2181 
   Size:        195    1924      62

  Fragments arranged by size: 

                1924     195      62
 
NcoI C'CATG_G

Cuts at:      0    1706    2181 
   Size:       1706     475
 
NspI r_CATG'y

Cuts at:      0    1620    1720    2181 
   Size:       1620     100     461

  Fragments arranged by size: 

                1620     461     100
 
PfoI T'CCnGG_A

Cuts at:      0     152    2181 
   Size:        152    2029
 
PmlI CAC'GTG

Cuts at:      0     821    2181 
   Size:        821    1360
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     995    1027    2181 
   Size:        995      32    1154

  Fragments arranged by size: 

                1154     995      32
 
PsiI TTA'TAA

Cuts at:      0     286    2181 
   Size:        286    1895
 
PstI C_TGCA'G

Cuts at:      0    1576    2181 
   Size:       1576     605
 
PvuII CAG'CTG

Cuts at:      0     195    2119    2181 
   Size:        195    1924      62

  Fragments arranged by size: 

                1924     195      62
 
SfcI C'TryA_G

Cuts at:      0    1572    2181 
   Size:       1572     609
 
StyI C'CwwG_G

Cuts at:      0    1706    2181 
   Size:       1706     475
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1051    1345    1561    2181 
   Size:       1051     294     216     620

  Fragments arranged by size: 

                1051     620     294     216
 
TatI w'GTAC_w

Cuts at:      0     171    2181 
   Size:        171    2010
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     231     263    2181 
   Size:        231      32    1918

  Fragments arranged by size: 

                1918     231      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1330    2181 
   Size:       1330     851
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     519    1005    2181 
   Size:        519     486    1176

  Fragments arranged by size: 

                1176     519     486
 
XmnI GAAnn'nnTTC

Cuts at:      0     398     488     793    1010    1048    1200    2181 
   Size:        398      90     305     217      38     152     981

  Fragments arranged by size: 

                 981     398     305     217     152      90      38
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AleI     AloI    AlwNI
     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI     BsmI    BsmBI Bsp1286I
    BspHI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII
   HincII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PfoI     PmlI     PpiI     PsiI     PstI    PvuII     SfcI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AhdI     AlfI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI    BfrBI     BmrI     BmtI     BplI    BpuEI    BsaBI
    BsaWI    BsiEI  BsiHKAI    BsiWI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmaI     ZraI
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