Restriction map for pF1KB5194
##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        AlwI        AlwNI       
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoT22I     
FauI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcsI          271  GAATTC               [GA]^AATT[CT]
AfaI          457  GTAC                 GT^AC
Alw44I        183  GTGCAC               G^TGCAC
AocI          394  CCTGAGG              CC^T[ACGT]AGG
ApaLI         183  GTGCAC               G^TGCAC
ApoI          271  GAATTC               [GA]^AATT[CT]
Asp700I       304  GAACTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         183  GTGCAC               G[AT]GC[AT]^C
AvaII         264  GGACC                G^G[AT]CC
BclI          377  TGATCA               T^GATCA
BmyI          183  GTGCAC               G[AGT]GC[ACT]^C
BsiHKAI       183  GTGCAC               G[AT]GC[AT]^C
BsmFI         263  GGGAC                GGGAC(10/14)
BsmI          150  GAATGC               GAATGC(1/-1)
Bsp1286I      183  GTGCAC               G[AGT]GC[ACT]^C
BssKI         453  CCAGG                ^CC[ACGT]GG
BstNI         453  CCAGG                CC^[AT]GG
Bsu36I        394  CCTGAGG              CC^T[ACGT]AGG
Cfr13I        264  GGACC                G^G[ACGT]CC
DpnII         378  GATC                 ^GATC
Eco81I        394  CCTGAGG              CC^T[ACGT]AGG
EcoRI         271  GAATTC               G^AATTC
EcoRII        453  CCAGG                ^CC[AT]GG
EcoRV         250  GATATC               GAT^ATC
EcoT14I       288  CCAAGG               C^C[AT][AT]GG
FbaI          377  TGATCA               T^GATCA
HincII        283  GTTGAC               GT[CT]^[GA]AC
HindII        283  GTTGAC               GT[CT]^[GA]AC
HinfI         402  GATTC                G^A[ACGT]TC
MboI          378  GATC                 ^GATC
MslI          193  CACAAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          453  CCAGG                CC^[AT]GG
NdeII         378  GATC                 ^GATC
NlaIV         263  GGGACC               GG[ACGT]^[ACGT]CC
NspI          355  ACATGC               [GA]CATG^[CT]
RsaI          457  GTAC                 GT^AC
Sau3AI        378  GATC                 ^GATC
Sau96I        264  GGACC                G^G[ACGT]CC
ScrFI         453  CCAGG                CC^[ACGT]GG
SspI          159  AATATT               AAT^ATT
StyI          288  CCAAGG               C^C[AT][AT]GG
TfiI          402  GATTC                G^A[AT]TC
Tsp45I         73  GTGAC                ^GT[GC]AC
XmnI          304  GAACTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BbvI          154  GCAGC                GCAGC(8/12)
              316  GCTGC               
BsaJI         288  CCAAGG               C^C[ACGT][ACGT]GG
              452  CCCAGG              
BsiYI         189  CCACCACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              255  CCCTGTTTGGG         
BslI          189  CCACCACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              255  CCCTGTTTGGG         
BsrI          130  CCAGT                ACTGG(1/-1)
              471  CCAGT               
BstF5I        108  GGATG                GGATG(2/0)
              198  GGATG               
DdeI          327  CTTAG                C^T[ACGT]AG
              395  CTGAG               
EarI          439  GAAGAG               CTCTTC(1/4)
              481  GAAGAG              
Fnu4HI        154  GCAGC                GC^[ACGT]GC
              316  GCTGC               
FokI          108  GGATG                GGATG(9/13)
              198  GGATG               
ItaI          154  GCAGC                GC^[ACGT]GC
              316  GCTGC               
Ksp632I       439  GAAGAG               CTCTTC(1/4)
              481  GAAGAG              
MaeIII         73  GTGAC                ^GT[ACGT]AC
              352  GTTAC               
NlaIII         87  CATG                 CATG^
              356  CATG                
TseI          154  GCAGC                G^C[AT]GC
              316  GCTGC               
TspRI         178  CAGTG                CA[GC]TG[ACGT][ACGT]^
              472  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

BseRI       MboII       MnlI        MseI        Tru9I       
Tsp509I     
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