Restriction map for
pF1KB5134
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI AocI ApaI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsiWI BsmBI BsmI BsoBI BspHI
BspLU11I BssHII BstBI BstEII BstPI
BstUI Bsu36I CelII CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HgaI HindIII HpaI KpnI
KspI MluI MvnI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PstI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 814 GTATAC GT^[AC][GT]AC
AccIII 468 TCCGGA T^CCGGA
AcyI 1852 GGCGCC G[GA]^CG[CT]C
AflIII 371 ACACGT A^C[GA][CT]GT
Alw44I 1054 GTGCAC G^TGCAC
AlwNI 1187 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 146 AGCGCT AGC^GCT
ApaLI 1054 GTGCAC G^TGCAC
BalI 1421 TGGCCA TGG^CCA
BbeI 1852 GGCGCC GGCGC^C
BbrPI 697 CACGTG CAC^GTG
BbsI 2011 GAAGAC GAAGAC(2/6)
BciVI 599 GTATCC GTATCC(6/5)
BpuAI 2011 GAAGAC GAAGAC(2/6)
BsaAI 697 CACGTG [CT]AC^GT[GA]
BsaBI 1860 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1852 GGCGCC G[GA]^CG[CT]C
BsaI 484 GAGACC GGTCTC(1/5)
BsaWI 468 TCCGGA [AT]^CCGG[AT]
BseAI 468 TCCGGA T^CCGGA
BsiEI 1263 CGATCG CG[GA][CT]^CG
Bsp1407I 593 TGTACA T^GTACA
BspDI 1261 ATCGAT AT^CGAT
BspEI 468 TCCGGA T^CCGGA
BsrBI 76 GAGCGG CCGCTC(-3/-3)
BsrDI 1079 GCAATG GCAATG(2/0)
BsrFI 1837 GCCGGC [GA]^CCGG[CT]
BsrGI 593 TGTACA T^GTACA
Bst1107I 814 GTATAC GTA^TAC
BstAPI 44 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 879 AGATCC [GA]^GATC[CT]
BstZ17I 814 GTATAC GTA^TAC
Cfr10I 1837 GCCGGC [GA]^CCGG[CT]
ClaI 1261 ATCGAT AT^CGAT
DraIII 697 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 146 AGCGCT AGC^GCT
EcoNI 1471 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 430 GAATTC G^AATTC
Hin1I 1852 GGCGCC G[GA]^CG[CT]C
HincII 1441 GTCAAC GT[CT]^[GA]AC
HindII 1441 GTCAAC GT[CT]^[GA]AC
KasI 1852 GGCGCC G^GCGCC
MamI 1860 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1526 CAATTG C^AATTG
MflI 879 AGATCC [GA]^GATC[CT]
MluNI 1421 TGGCCA TGG^CCA
MroI 468 TCCGGA T^CCGGA
MscI 1421 TGGCCA TGG^CCA
MspA1I 1187 CAGCTG C[AC]G^C[GT]G
MunI 1526 CAATTG C^AATTG
NaeI 1837 GCCGGC GCC^GGC
NarI 1852 GGCGCC GG^CGCC
NcoI 105 CCATGG C^CATGG
NdeI 730 CATATG CA^TATG
NgoMIV 1837 GCCGGC G^CCGGC
PmaCI 697 CACGTG CAC^GTG
PmlI 697 CACGTG CAC^GTG
PshAI 774 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1263 CGATCG CGAT^CG
PvuII 1187 CAGCTG CAG^CTG
SacI 2265 GAGCTC GAGCT^C
SfoI 1852 GGCGCC GGC^GCC
SspBI 593 TGTACA T^GTACA
SspI 916 AATATT AAT^ATT
StuI 1820 AGGCCT AGG^CCT
XhoII 879 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 430 GAATTC [GA]^AATT[CT]
1617 AAATTT
ApoI 430 GAATTC [GA]^AATT[CT]
1617 AAATTT
AseI 454 ATTAAT AT^TAAT
1297 ATTAAT
AsnI 454 ATTAAT AT^TAAT
1297 ATTAAT
Asp700I 171 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1600 GAAATCATTC
BclI 1497 TGATCA T^GATCA
1718 TGATCA
BpmI 2041 CTCCAG CTGGAG(16/14)
415 CTGGAG
BsgI 478 CTGCAC GTGCAG(16/14)
1541 GTGCAG
BsmAI 484 GAGAC GTCTC(1/5)
1631 GAGAC
BspMI 89 ACCTGC ACCTGC(4/8)
135 GCAGGT
BssSI 481 CACGAG CACGAG(-5/-1)
2166 CACGAG
EarI 355 GAAGAG CTCTTC(1/4)
863 GAAGAG
FbaI 1497 TGATCA T^GATCA
1718 TGATCA
Ksp632I 355 GAAGAG CTCTTC(1/4)
863 GAAGAG
PflMI 883 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1303 CCAGAGGATGG
PshBI 454 ATTAAT AT^TAAT
1297 ATTAAT
SphI 44 GCATGC GCATG^C
1215 GCATGC
Van91I 883 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1303 CCAGAGGATGG
XmnI 171 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1600 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI