Restriction map for pF1KB4967
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AocI        AscI        Asp718I     AspI        
AviII       AvrII       BbeI        BclI        BlnI        
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BsrFI       BssHII      BstUI       Bsu36I      Cfr10I      
ClaI        CpoI        EagI        EclXI       Eco52I      
Eco81I      EcoRV       FbaI        FseI        FspI        
HindIII     HpaI        KasI        KpnI        KspI        
MluI        MroI        MvnI        NaeI        NarI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PmeI        Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfoI        SgrAI       SnaBI       Sse8387I    SspI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflII         724  CTTAAG               C^TTAAG
AflIII       1853  ACATGT               A^C[GA][CT]GT
AhdI         5916  GACCAGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      5207  AGCGCT               AGC^GCT
ApaI         5758  GGGCCC               GGGCC^C
AspEI        5916  GACCAGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         4711  CCCGGG               C^[CT]CG[GA]G
BbrPI        5830  CACGTG               CAC^GTG
BfrI          724  CTTAAG               C^TTAAG
BglII        3767  AGATCT               A^GATCT
BsaAI        5830  CACGTG               [CT]AC^GT[GA]
BsaWI        3395  ACCGGA               [AT]^CCGG[AT]
BsoBI        4711  CCCGGG               C^[CT]CG[GA]G
BspLU11I     1853  ACATGT               A^CATGT
BsrBI        1877  GAGCGG               CCGCTC(-3/-3)
BssSI        3557  CTCGTG               CACGAG(-5/-1)
BstBI        1736  TTCGAA               TT^CGAA
BstEII       5712  GGTGACC              G^GT[ACGT]ACC
BstPI        5712  GGTGACC              G^GT[ACGT]ACC
Eam1105I     5916  GACCAGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     5207  AGCGCT               AGC^GCT
EcoO65I      5712  GGTGACC              G^GT[ACGT]ACC
NheI         7129  GCTAGC               G^CTAGC
NspV         1736  TTCGAA               TT^CGAA
PmaCI        5830  CACGTG               CAC^GTG
PmlI         5830  CACGTG               CAC^GTG
PshAI        4230  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2228  GAGCTC               GAGCT^C
ScaI         3091  AGTACT               AGT^ACT
SfiI          312  GGCCAGCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1736  TTCGAA               TT^CGAA
SmaI         4711  CCCGGG               CCC^GGG
XbaI         3903  TCTAGA               T^CTAGA
XmaCI        4711  CCCGGG               C^CCGGG
XmaI         4711  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI           72  GGCGTC               G[GA]^CG[CT]C
             5601  GACGCC              
Alw44I       3546  GTGCAC               G^TGCAC
             3836  GTGCAC              
ApaLI        3546  GTGCAC               G^TGCAC
             3836  GTGCAC              
AseI          138  ATTAAT               AT^TAAT
              181  ATTAAT              
AsnI          138  ATTAAT               AT^TAAT
              181  ATTAAT              
BcgI         5957  CGACAGGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2733  GCAGCAGCCTCG        
BsaBI         178  GATATTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4840  GATCCTGATC          
BsaHI          72  GGCGTC               G[GA]^CG[CT]C
             5601  GACGCC              
BsmBI        5809  GAGACG               CGTCTC(1/5)
             6549  GAGACG              
Bsp1407I     1216  TGTACA               T^GTACA
             3739  TGTACA              
BspHI         572  TCATGA               T^CATGA
             4242  TCATGA              
BsrGI        1216  TGTACA               T^GTACA
             3739  TGTACA              
Bst1107I     3412  GTATAC               GTA^TAC
             4177  GTATAC              
BstZ17I      3412  GTATAC               GTA^TAC
             4177  GTATAC              
EcoT22I      3760  ATGCAT               ATGCA^T
             4126  ATGCAT              
Hin1I          72  GGCGTC               G[GA]^CG[CT]C
             5601  GACGCC              
MaeII        5831  ACGT                 A^CGT
             7016  ACGT                
MamI          178  GATATTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4840  GATCCTGATC          
MfeI          584  CAATTG               C^AATTG
             5253  CAATTG              
MunI          584  CAATTG               C^AATTG
             5253  CAATTG              
NdeI         1829  CATATG               CA^TATG
             6847  CATATG              
NsiI         3760  ATGCAT               ATGCA^T
             4126  ATGCAT              
PshBI         138  ATTAAT               AT^TAAT
              181  ATTAAT              
RcaI          572  TCATGA               T^CATGA
             4242  TCATGA              
SpeI          418  ACTAGT               A^CTAGT
             2869  ACTAGT              
SspBI        1216  TGTACA               T^GTACA
             3739  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  129 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BamHI       BanI        BanII       
BbsI        BbvCI       BbvI        BciVI       BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI        
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