Restriction map for pF1KB4815
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AspEI       AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BsaBI       
BsaWI       BseAI       BspEI       BsrFI       BssHII      
BssSI       BstBI       BstUI       Bsu36I      Cfr10I      
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I       1246  GGTACC               G^GTACC
AseI         1948  ATTAAT               AT^TAAT
AsnI         1948  ATTAAT               AT^TAAT
Asp718I      1246  GGTACC               G^GTACC
AspI         2689  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         504  GGATCC               G^GATCC
BlpI         1831  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1831  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2108  TACGTG               [CT]AC^GT[GA]
BsiEI        4136  CGATCG               CG[GA][CT]^CG
BsiWI        2190  CGTACG               C^GTACG
BsmBI        4360  GAGACG               CGTCTC(1/5)
BspDI        3172  ATCGAT               AT^CGAT
BspLU11I      786  ACATGT               A^CATGT
BsrBI        4116  CCGCTC               CCGCTC(-3/-3)
Bst1107I      493  GTATAC               GTA^TAC
BstAPI       4035  GCAGACTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       493  GTATAC               GTA^TAC
CelII        1831  GCTGAGC              GC^T[ACGT]AGC
ClaI         3172  ATCGAT               AT^CGAT
DraIII       2286  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        4254  CCTTGATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1085  GAATTC               G^AATTC
FauI         2494  CCCGC                CCCGC(4/6)
HaeII        3551  AGCGCC               [GA]GCGC^[CT]
KpnI         1246  GGTACC               GGTAC^C
MfeI         4376  CAATTG               C^AATTG
MunI         4376  CAATTG               C^AATTG
PaeR7I       3155  CTCGAG               C^TCGAG
PflFI        2689  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2764  CCATCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4221  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1948  ATTAAT               AT^TAAT
PstI         3898  CTGCAG               CTGCA^G
PvuI         4136  CGATCG               CGAT^CG
SapI         2170  GAAGAGC              GCTCTTC(1/4)
SpeI          489  ACTAGT               A^CTAGT
SspI         3991  AATATT               AAT^ATT
StuI         2417  AGGCCT               AGG^CCT
Tth111I      2689  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2764  CCATCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         3155  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII        786  ACATGT               A^C[GA][CT]GT
             2109  ACGTGT              
Asp700I       457  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              667  GAATTTGTTC          
AvaI         2831  CCCGAG               C^[CT]CG[GA]G
             3155  CTCGAG              
BalI         1410  TGGCCA               TGG^CCA
             1691  TGGCCA              
BbvCI        2254  GCTGAGG              CCTCAGC(-5/-2)
             3610  GCTGAGG             
BcnI         1643  CCGGG                CC^[GC]GG
             2466  CCGGG               
BsmI         3726  GAATGC               GAATGC(1/-1)
             2029  GCATTC              
BsoBI        2831  CCCGAG               C^[CT]CG[GA]G
             3155  CTCGAG              
Bsp1407I     1212  TGTACA               T^GTACA
             1997  TGTACA              
BspHI        1395  TCATGA               T^CATGA
             1817  TCATGA              
BsrGI        1212  TGTACA               T^GTACA
             1997  TGTACA              
BstEII       3031  GGTTACC              G^GT[ACGT]ACC
             3472  GGTAACC             
BstPI        3031  GGTTACC              G^GT[ACGT]ACC
             3472  GGTAACC             
EaeI         1410  TGGCCA               [CT]^GGCC[GA]
             1691  TGGCCA              
EcoO65I      3031  GGTTACC              G^GT[ACGT]ACC
             3472  GGTAACC             
EcoT22I      2089  ATGCAT               ATGCA^T
             2460  ATGCAT              
MluNI        1410  TGGCCA               TGG^CCA
             1691  TGGCCA              
MscI         1410  TGGCCA               TGG^CCA
             1691  TGGCCA              
NciI         1643  CCGGG                CC^[GC]GG
             2466  CCGGG               
NdeI          764  CATATG               CA^TATG
             3201  CATATG              
NsiI         2089  ATGCAT               ATGCA^T
             2460  ATGCAT              
NspI          786  ACATGT               [GA]CATG^[CT]
             2163  GCATGT              
PpuMI        1874  AGGACCT              [GA]G^G[AT]CC[CT]
             3677  AGGACCT             
PvuII         928  CAGCTG               CAG^CTG
             2479  CAGCTG              
RcaI         1395  TCATGA               T^CATGA
             1817  TCATGA              
ScaI         2198  AGTACT               AGT^ACT
             3495  AGTACT              
SfcI         3072  CTACAG               C^T[GA][CT]AG
             3898  CTGCAG              
SspBI        1212  TGTACA               T^GTACA
             1997  TGTACA              
XmnI          457  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              667  GAATTTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com