Restriction map for pF1KB4815
 (Linear) (Six-base) MAPSORT of: KIBB4815.seq  Check: 4068  from: 1  to: 4422



KIBB4815.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1092    1760    2236    4422 
   Size:       1092     668     476    2186

  Fragments arranged by size: 

                2186    1092     668     476
 
AccI GT'mk_AC

Cuts at:      0     494    2106    3965    4422 
   Size:        494    1612    1859     457

  Fragments arranged by size: 

                1859    1612     494     457
 
Acc65I G'GTAC_C

Cuts at:      0    1246    4422 
   Size:       1246    3176
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     952    1489    1698    2120    2339    2648    2741    2804 
   Size:        952     537     209     422     219     309      93      63

Cuts at:   2804    3157    3830    4195    4422 
   Size:        353     673     365     227

  Fragments arranged by size: 

                 952     673     537     422     365     353     309     227
                 219     209      93      63
 
AflIII A'CryG_T

Cuts at:      0     786    2109    4422 
   Size:        786    1323    2313

  Fragments arranged by size: 

                2313    1323     786
 
AleI CACnn'nnGTG

Cuts at:      0    3763    4422 
   Size:       3763     659
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3743    4422 
   Size:       3743     679
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     286     318    4422 
   Size:        286      32    4104

  Fragments arranged by size: 

                4104     286      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      76     175    2275    2671    4041    4278    4422 
   Size:         76      99    2100     396    1370     237     144

  Fragments arranged by size: 

                2100    1370     396     237     144      99      76
 
ApoI r'AATT_y

Cuts at:      0     337     433     667     865    1085    2604    3865    4422 
   Size:        337      96     234     198     220    1519    1261     557

  Fragments arranged by size: 

                1519    1261     557     337     234     220     198      96
            
 
AseI AT'TA_AT

Cuts at:      0    1949    4422 
   Size:       1949    2473
 
AvaI C'yCGr_G

Cuts at:      0    2831    3155    4422 
   Size:       2831     324    1267

  Fragments arranged by size: 

                2831    1267     324
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1955    1988    4422 
   Size:       1955      33    2434

  Fragments arranged by size: 

                2434    1955      33
 
BamHI G'GATC_C

Cuts at:      0     504    4422 
   Size:        504    3918
 
BanI G'GyrC_C

Cuts at:      0    1246    1453    2658    3570    4422 
   Size:       1246     207    1205     912     852

  Fragments arranged by size: 

                1246    1205     912     852     207
 
BanII G_rGCy'C

Cuts at:      0     593     604    1388    1838    2387    3247    4422 
   Size:        593      11     784     450     549     860    1175

  Fragments arranged by size: 

                1175     860     784     593     549     450      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     272     915    1559    1925    3145    3403    3836    4422 
   Size:        272     643     644     366    1220     258     433     586

  Fragments arranged by size: 

                1220     644     643     586     433     366     272     258
            
 
BbvCI CC'TCA_GC

Cuts at:      0    2255    3611    4422 
   Size:       2255    1356     811

  Fragments arranged by size: 

                2255    1356     811
 
BfrBI ATG'CAT

Cuts at:      0    2091    2462    4422 
   Size:       2091     371    1960

  Fragments arranged by size: 

                2091    1960     371
 
BglII A'GATC_T

Cuts at:      0    4201    4320    4407    4422 
   Size:       4201     119      87      15

  Fragments arranged by size: 

                4201     119      87      15
 
BlpI GC'TnA_GC

Cuts at:      0    1832    4422 
   Size:       1832    2590
 
BmgBI CAC'GTC

Cuts at:      0    1650    4422 
   Size:       1650    2772
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1532    2506    3824    4306    4422 
   Size:       1532     974    1318     482     116

  Fragments arranged by size: 

                1532    1318     974     482     116
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250    1952    2881    2975    3349    3403    3955    4422 
   Size:        250    1702     929      94     374      54     552     467

  Fragments arranged by size: 

                1702     929     552     467     374     250      94      54
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2255    3611    4422 
   Size:       2255    1356     811

  Fragments arranged by size: 

                2255    1356     811
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544    1402    2413    2621    3484    4422 
   Size:        544     858    1011     208     863     938

  Fragments arranged by size: 

                1011     938     863     858     544     208
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     118    2179    3912    4161    4422 
   Size:        118    2061    1733     249     261

  Fragments arranged by size: 

                2061    1733     261     249     118
 
BsaAI yAC'GTr

Cuts at:      0    2110    4422 
   Size:       2110    2312
 
BsaHI Gr'CG_yC

Cuts at:      0      65    1127    1843    4422 
   Size:         65    1062     716    2579

  Fragments arranged by size: 

                2579    1062     716      65
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     286     316    1165    1195    1499    1529    1715    1745 
   Size:        286      30     849      30     304      30     186      30

Cuts at:   1745    1752    1782    1950    1980    2255    2285    4422 
   Size:          7      30     168      30     275      30    2137

  Fragments arranged by size: 

                2137     849     304     286     275     186     168      30
                  30      30      30      30      30      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     307    1760    3293    3628    3735    4422 
   Size:         31     276    1453    1533     335     107     687

  Fragments arranged by size: 

                1533    1453     687     335     276     107      31
 
BseYI C'CCAG_C

Cuts at:      0    1461    1734    3656    4422 
   Size:       1461     273    1922     766

  Fragments arranged by size: 

                1922    1461     766     273
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2497    3809    4054    4422 
   Size:       2497    1312     245     368

  Fragments arranged by size: 

                2497    1312     368     245
 
BsiEI CG_ry'CG

Cuts at:      0    4139    4422 
   Size:       4139     283
 
BsiHKAI G_wGCw'C

Cuts at:      0     650    1838    2387    3247    3638    4422 
   Size:        650    1188     549     860     391     784

  Fragments arranged by size: 

                1188     860     784     650     549     391
 
BsiWI C'GTAC_G

Cuts at:      0    2190    4422 
   Size:       2190    2232
 
BsmI GAATG_Cn'

Cuts at:      0    2029    3732    4422 
   Size:       2029    1703     690

  Fragments arranged by size: 

                2029    1703     690
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4354    4422 
   Size:       4354      68
 
Bsp1286I G_dGCh'C

Cuts at:      0     593     604     650    1388    1838    2387    3247    3638 
   Size:        593      11      46     738     450     549     860     391

Cuts at:   3638    4422 
   Size:        784

  Fragments arranged by size: 

                 860     784     738     593     549     450     391      46
                  11
 
BspHI T'CATG_A

Cuts at:      0    1395    1817    4422 
   Size:       1395     422    2605

  Fragments arranged by size: 

                2605    1395     422
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1092    1760    1868    2236    3460    3894    4422 
   Size:       1092     668     108     368    1224     434     528

  Fragments arranged by size: 

                1224    1092     668     528     434     368     108
 
BsrBI CCG'CTC

Cuts at:      0    4118    4422 
   Size:       4118     304
 
BsrDI GCAATG_nn'

Cuts at:      0    1598    3372    3910    4422 
   Size:       1598    1774     538     512

  Fragments arranged by size: 

                1774    1598     538     512
 
BsrGI T'GTAC_A

Cuts at:      0    1212    1997    4422 
   Size:       1212     785    2425

  Fragments arranged by size: 

                2425    1212     785
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4041    4422 
   Size:       4041     381
 
BstEII G'GTnAC_C

Cuts at:      0    3031    3472    4422 
   Size:       3031     441     950

  Fragments arranged by size: 

                3031     950     441
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     360    1242    1701    4422 
   Size:        360     882     459    2721

  Fragments arranged by size: 

                2721     882     459     360
 
BstYI r'GATC_y

Cuts at:      0     504     974    2216    4201    4320    4407    4422 
   Size:        504     470    1242    1985     119      87      15

  Fragments arranged by size: 

                1985    1242     504     470     119      87      15
 
BstZ17I GTA'TAC

Cuts at:      0     495    4422 
   Size:        495    3927
 
BtgI C'CryG_G

Cuts at:      0     449     659     834     888    1193    2435    3417    4422 
   Size:        449     210     175      54     305    1242     982    1005

  Fragments arranged by size: 

                1242    1005     982     449     305     210     175      54
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     507    3303    4422 
   Size:        507    2796    1119

  Fragments arranged by size: 

                2796    1119     507
 
BtsI GCAGTG_nn'

Cuts at:      0    1337    2345    2535    3253    3442    4422 
   Size:       1337    1008     190     718     189     980

  Fragments arranged by size: 

                1337    1008     980     718     190     189
 
ClaI AT'CG_AT

Cuts at:      0    3173    4422 
   Size:       3173    1249
 
DraIII CAC_nnn'GTG

Cuts at:      0    2291    4422 
   Size:       2291    2131
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      70     382    2102    3538    3840    4422 
   Size:         70     312    1720    1436     302     582

  Fragments arranged by size: 

                1720    1436     582     312     302      70
 
EaeI y'GGCC_r

Cuts at:      0    1410    1691    4422 
   Size:       1410     281    2731

  Fragments arranged by size: 

                2731    1410     281
 
EarI CTCTTCn'nnn_

Cuts at:      0     223     725     928    1319    2136    2165    2346    4004 
   Size:        223     502     203     391     817      29     181    1658

Cuts at:   4004    4422 
   Size:        418

  Fragments arranged by size: 

                1658     817     502     418     391     223     203     181
                  29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     250    3294    4422 
   Size:        250    3044    1128

  Fragments arranged by size: 

                3044    1128     250
 
EcoICRI GAG'CTC

Cuts at:      0    1836    2385    3245    4422 
   Size:       1836     549     860    1177

  Fragments arranged by size: 

                1836    1177     860     549
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     952    1489    1698    1952    2120    2339    2648 
   Size:        250     702     537     209     254     168     219     309

Cuts at:   2648    2741    2804    2881    2975    3157    3349    3403    3830 
   Size:         93      63      77      94     182     192      54     427

Cuts at:   3830    3955    4195    4422 
   Size:        125     240     227

  Fragments arranged by size: 

                 702     537     427     309     254     250     240     227
                 219     209     192     182     168     125      94      93
                  77      63      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4258    4422 
   Size:       4258     164
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1875    2303    3678    4422 
   Size:       1875     428    1375     744

  Fragments arranged by size: 

                1875    1375     744     428
 
EcoRI G'AATT_C

Cuts at:      0    1085    4422 
   Size:       1085    3337
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1497    4422 
   Size:       1497    2925
 
HaeII r_GCGC'y

Cuts at:      0    3555    4422 
   Size:       3555     867
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1375    1407    1499    1531    2255    2287    2321    2353 
   Size:       1375      32      92      32     724      32      34      32

Cuts at:   2353    2825    2857    2986    3018    3149    3181    3610    3642 
   Size:        472      32     129      32     131      32     429      32

Cuts at:   3642    3670    3702    4029    4061    4249    4281    4422 
   Size:         28      32     327      32     188      32     141

  Fragments arranged by size: 

                1375     724     472     429     327     188     141     131
                 129      92      34      32      32      32      32      32
                  32      32      32      32      32      32      28
 
KpnI G_GTAC'C

Cuts at:      0    1250    4422 
   Size:       1250    3172
 
MfeI C'AATT_G

Cuts at:      0    4376    4422 
   Size:       4376      46
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     851    4151    4422 
   Size:        851    3300     271

  Fragments arranged by size: 

                3300     851     271
 
MscI TGG'CCA

Cuts at:      0    1412    1693    4422 
   Size:       1412     281    2729

  Fragments arranged by size: 

                2729    1412     281
 
MslI CAynn'nnrTG

Cuts at:      0     358    3062    3763    3943    4422 
   Size:        358    2704     701     180     479

  Fragments arranged by size: 

                2704     701     479     358     180
 
MspA1I CmG'CkG

Cuts at:      0     930    1765    2481    4422 
   Size:        930     835     716    1941

  Fragments arranged by size: 

                1941     930     835     716
 
NcoI C'CATG_G

Cuts at:      0     449     888    3417    4422 
   Size:        449     439    2529    1005

  Fragments arranged by size: 

                2529    1005     449     439
 
NdeI CA'TA_TG

Cuts at:      0     765    3202    4422 
   Size:        765    2437    1220

  Fragments arranged by size: 

                2437    1220     765
 
NsiI A_TGCA'T

Cuts at:      0    2093    2464    4422 
   Size:       2093     371    1958

  Fragments arranged by size: 

                2093    1958     371
 
NspI r_CATG'y

Cuts at:      0     790    2167    4422 
   Size:        790    1377    2255

  Fragments arranged by size: 

                2255    1377     790
 
PasI CC'CwG_GG

Cuts at:      0    1891    3732    4422 
   Size:       1891    1841     690

  Fragments arranged by size: 

                1891    1841     690
 
PciI A'CATG_T

Cuts at:      0     786    4422 
   Size:        786    3636
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2770    4422 
   Size:       2770    1652
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     286     318    1512    1544    4422 
   Size:        286      32    1194      32    2878

  Fragments arranged by size: 

                2878    1194     286      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1875    3678    4422 
   Size:       1875    1803     744

  Fragments arranged by size: 

                1875    1803     744
 
PshAI GACnn'nnGTC

Cuts at:      0    4225    4422 
   Size:       4225     197
 
PspXI vC'TCGA_Gb

Cuts at:      0    3155    4422 
   Size:       3155    1267
 
PstI C_TGCA'G

Cuts at:      0    3902    4422 
   Size:       3902     520
 
PvuI CG_AT'CG

Cuts at:      0    4139    4422 
   Size:       4139     283
 
PvuII CAG'CTG

Cuts at:      0     930    2481    4422 
   Size:        930    1551    1941

  Fragments arranged by size: 

                1941    1551     930
 
SacI G_AGCT'C

Cuts at:      0    1838    2387    3247    4422 
   Size:       1838     549     860    1175

  Fragments arranged by size: 

                1838    1175     860     549
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2165    4422 
   Size:       2165    2257
 
ScaI AGT'ACT

Cuts at:      0    2200    3497    4422 
   Size:       2200    1297     925

  Fragments arranged by size: 

                2200    1297     925
 
SfcI C'TryA_G

Cuts at:      0    3072    3898    4422 
   Size:       3072     826     524

  Fragments arranged by size: 

                3072     826     524
 
SmlI C'TyrA_G

Cuts at:      0     559    1381    2428    2636    3155    3499    4422 
   Size:        559     822    1047     208     519     344     923

  Fragments arranged by size: 

                1047     923     822     559     519     344     208
 
SpeI A'CTAG_T

Cuts at:      0     489    4422 
   Size:        489    3933
 
SspI AAT'ATT

Cuts at:      0    3993    4422 
   Size:       3993     429
 
StuI AGG'CCT

Cuts at:      0    2419    4422 
   Size:       2419    2003
 
StyI C'CwwG_G

Cuts at:      0     188     449     888     917    3005    3417    3566    4422 
   Size:        188     261     439      29    2088     412     149     856

  Fragments arranged by size: 

                2088     856     439     412     261     188     149      29
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1296    3766    4422 
   Size:       1296    2470     656

  Fragments arranged by size: 

                2470    1296     656
 
TatI w'GTAC_w

Cuts at:      0     690    1212    1978    1997    2198    2955    3495    4385 
   Size:        690     522     766      19     201     757     540     890

Cuts at:   4385    4422 
   Size:         37

  Fragments arranged by size: 

                 890     766     757     690     540     522     201      37
                  19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     980    1012    1526    1558    1715    1747    1752    1784 
   Size:        980      32     514      32     157      32       5      32

Cuts at:   1784    1950    1982    4422 
   Size:        166      32    2440

  Fragments arranged by size: 

                2440     980     514     166     157      32      32      32
                  32      32       5
 
Tth111I GACn'n_nGTC

Cuts at:      0    2692    4422 
   Size:       2692    1730
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     550    1239    2905    3324    3444    3573    4422 
   Size:        550     689    1666     419     120     129     849

  Fragments arranged by size: 

                1666     849     689     550     419     129     120
 
XhoI C'TCGA_G

Cuts at:      0    3155    4422 
   Size:       3155    1267
 
XmnI GAAnn'nnTTC

Cuts at:      0     461     671    4422 
   Size:        461     210    3751

  Fragments arranged by size: 

                3751     461     210
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI    BfrBI    BglII     BlpI    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI     PasI     PciI    PflMI     PpiI
    PpuMI    PshAI    PspXI     PstI     PvuI    PvuII     SacI     SapI
     ScaI     SfcI     SmlI     SpeI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BbeI     BcgI    BciVI     BclI     BglI
 Bme1580I     BmtI     BplI    BsaBI    BsaWI    BspEI    BsrFI   BssHII
    BssSI    BstBI   Bsu36I    CspCI     DraI     EagI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     MluI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PfoI     PmeI
     PmlI     PsiI   PspOMI     PsrI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SwaI     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com