Restriction map for pF1KB4796
 (Linear) (Six-base) MAPSORT of: KIBB4796.seq  Check: 6973  from: 1  to: 669



KIBB4796.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

Acc65I G'GTAC_C

Cuts at:      0     589     669 
   Size:        589      80
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     669 
   Size:        484     185
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     377     669 
   Size:        377     292
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     329     361     669 
   Size:        329      32     308

  Fragments arranged by size: 

                 329     308      32
 
ApoI r'AATT_y

Cuts at:      0      92     310     669 
   Size:         92     218     359

  Fragments arranged by size: 

                 359     218      92
 
AseI AT'TA_AT

Cuts at:      0     470     669 
   Size:        470     199
 
BanI G'GyrC_C

Cuts at:      0     589     669 
   Size:        589      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      53     669 
   Size:         53     616
 
BfrBI ATG'CAT

Cuts at:      0     185     669 
   Size:        185     484
 
BmgBI CAC'GTC

Cuts at:      0       9     669 
   Size:          9     660
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     357     541     669 
   Size:         37     320     184     128

  Fragments arranged by size: 

                 320     184     128      37
 
BsaHI Gr'CG_yC

Cuts at:      0      50     669 
   Size:         50     619
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     329     359     669 
   Size:        329      30     310

  Fragments arranged by size: 

                 329     310      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     669 
   Size:         70     599
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     641     669 
   Size:        401     240      28

  Fragments arranged by size: 

                 401     240      28
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      69     669 
   Size:         69     600
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     648     669 
   Size:        648      21
 
BstYI r'GATC_y

Cuts at:      0     368     669 
   Size:        368     301
 
EarI CTCTTCn'nnn_

Cuts at:      0     601     617     669 
   Size:        601      16      52

  Fragments arranged by size: 

                 601      52      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     357     484     541     669 
   Size:         37     320     127      57     128

  Fragments arranged by size: 

                 320     128     127      57      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     233     265     669 
   Size:        233      32     404

  Fragments arranged by size: 

                 404     233      32
 
KpnI G_GTAC'C

Cuts at:      0     593     669 
   Size:        593      76
 
MslI CAynn'nnrTG

Cuts at:      0     359     646     669 
   Size:        359     287      23

  Fragments arranged by size: 

                 359     287      23
 
NsiI A_TGCA'T

Cuts at:      0     187     669 
   Size:        187     482
 
PfoI T'CCnGG_A

Cuts at:      0     251     669 
   Size:        251     418
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     329     361     669 
   Size:        329      32     308

  Fragments arranged by size: 

                 329     308      32
 
SfcI C'TryA_G

Cuts at:      0     154     669 
   Size:        154     515
 
SspI AAT'ATT

Cuts at:      0     198     669 
   Size:        198     471
 
TatI w'GTAC_w

Cuts at:      0     150     425     669 
   Size:        150     275     244

  Fragments arranged by size: 

                 275     244     150
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     AloI     ApoI     AseI     BanI     BbsI
    BfrBI    BmgBI     BpmI    BsaHI    BsaXI    BseRI     BsgI    BsmBI
    BstXI    BstYI     EarI  Eco57MI    Hin4I     KpnI     MslI     NsiI
     PfoI     PpiI     SfcI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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