Restriction map for pF1KB4399
 (Linear) (Six-base) MAPSORT of: KIBB4399.seq  Check: 1292  from: 1  to: 6984



KIBB4399.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4691    5546    5946    6984 
   Size:       4691     855     400    1038

  Fragments arranged by size: 

                4691    1038     855     400
 
Acc65I G'GTAC_C

Cuts at:      0    1796    6984 
   Size:       1796    5188
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     694    1014    1795    2203    2284    3560    4282    4888 
   Size:        694     320     781     408      81    1276     722     606

Cuts at:   4888    4975    5044    5709    5859    6984 
   Size:         87      69     665     150    1125

  Fragments arranged by size: 

                1276    1125     781     722     694     665     606     408
                 320     150      87      81      69
 
AfeI AGC'GCT

Cuts at:      0    6190    6984 
   Size:       6190     794
 
AflIII A'CryG_T

Cuts at:      0    5063    6984 
   Size:       5063    1921
 
AleI CACnn'nnGTG

Cuts at:      0     805    6984 
   Size:        805    6179
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3323    4178    4543    4907    5389    6984 
   Size:       3323     855     365     364     482    1595

  Fragments arranged by size: 

                3323    1595     855     482     365     364
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     385     417    6984 
   Size:        385      32    6567

  Fragments arranged by size: 

                6567     385      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1020    1184    1461    1545    2475    2529    2823    3060 
   Size:       1020     164     277      84     930      54     294     237

Cuts at:   3060    3135    3207    3286    3297    3426    3613    4566    4629 
   Size:         75      72      79      11     129     187     953      63

Cuts at:   4629    4737    4947    5376    5661    5882    6375    6605    6897 
   Size:        108     210     429     285     221     493     230     292

Cuts at:   6897    6984 
   Size:         87

  Fragments arranged by size: 

                1020     953     930     493     429     294     292     285
                 277     237     230     221     210     187     164     129
                 108      87      84      79      75      72      63      54
                  11
 
ApaI G_GGCC'C

Cuts at:      0    2340    4941    6581    6984 
   Size:       2340    2601    1640     403

  Fragments arranged by size: 

                2601    2340    1640     403
 
ApaLI G'TGCA_C

Cuts at:      0    2866    5509    6984 
   Size:       2866    2643    1475

  Fragments arranged by size: 

                2866    2643    1475
 
ApoI r'AATT_y

Cuts at:      0    5713    6984 
   Size:       5713    1271
 
AvaI C'yCGr_G

Cuts at:      0     206    1570    4218    5207    5461    6042    6342    6984 
   Size:        206    1364    2648     989     254     581     300     642

  Fragments arranged by size: 

                2648    1364     989     642     581     300     254     206
            
 
AvrII C'CTAG_G

Cuts at:      0    4398    6984 
   Size:       4398    2586
 
BamHI G'GATC_C

Cuts at:      0    3617    6984 
   Size:       3617    3367
 
BanI G'GyrC_C

Cuts at:      0    1559    1796    2129    3335    4323    4679    4852    5416 
   Size:       1559     237     333    1206     988     356     173     564

Cuts at:   5416    5681    6984 
   Size:        265    1303

  Fragments arranged by size: 

                1559    1303    1206     988     564     356     333     265
                 237     173
 
BanII G_rGCy'C

Cuts at:      0     403    1241    1440    2057    2340    2486    3587    3857 
   Size:        403     838     199     617     283     146    1101     270

Cuts at:   3857    4141    4693    4814    4941    5354    5534    6089    6452 
   Size:        284     552     121     127     413     180     555     363

Cuts at:   6452    6497    6581    6984 
   Size:         45      84     403

  Fragments arranged by size: 

                1101     838     617     555     552     413     403     403
                 363     284     283     270     199     180     146     127
                 121      84      45
 
BbeI G_GCGC'C

Cuts at:      0    1563    4856    5685    6984 
   Size:       1563    3293     829    1299

  Fragments arranged by size: 

                3293    1563    1299     829
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     562    1697    2698    4117    6984 
   Size:        562    1135    1001    1419    2867

  Fragments arranged by size: 

                2867    1419    1135    1001     562
 
BbvCI CC'TCA_GC

Cuts at:      0    3550    3707    4787    6062    6772    6984 
   Size:       3550     157    1080    1275     710     212

  Fragments arranged by size: 

                3550    1275    1080     710     212     157
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     187     197     221     231     849     883    1396    1430 
   Size:        187      10      24      10     618      34     513      34

Cuts at:   1430    5656    5690    6984 
   Size:       4226      34    1294

  Fragments arranged by size: 

                4226    1294     618     513     187      34      34      34
                  24      10      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3432    6984 
   Size:       3432    3552
 
BglI GCCn_nnn'nGGC

Cuts at:      0     734    2335    3022    3535    3793    4753    4936    5488 
   Size:        734    1601     687     513     258     960     183     552

Cuts at:   5488    6058    6181    6984 
   Size:        570     123     803

  Fragments arranged by size: 

                1601     960     803     734     687     570     552     513
                 258     183     123
 
BglII A'GATC_T

Cuts at:      0    1973    2084    6984 
   Size:       1973     111    4900

  Fragments arranged by size: 

                4900    1973     111
 
BlpI GC'TnA_GC

Cuts at:      0     105     519     722    1864    3287    4256    6984 
   Size:        105     414     203    1142    1423     969    2728

  Fragments arranged by size: 

                2728    1423    1142     969     414     203     105
 
Bme1580I G_kGCm'C

Cuts at:      0    2132    2340    2870    3338    4682    4941    5513    6581 
   Size:       2132     208     530     468    1344     259     572    1068

Cuts at:   6581    6984 
   Size:        403

  Fragments arranged by size: 

                2132    1344    1068     572     530     468     403     259
                 208
 
BmgBI CAC'GTC

Cuts at:      0    3009    5031    5469    5571    6762    6984 
   Size:       3009    2022     438     102    1191     222

  Fragments arranged by size: 

                3009    2022    1191     438     222     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4026    4497    6275    6434    6984 
   Size:       4026     471    1778     159     550

  Fragments arranged by size: 

                4026    1778     550     471     159
 
BmtI G_CTAG'C

Cuts at:      0     931    6984 
   Size:        931    6053
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     300    4511    6984 
   Size:        300    4211    2473

  Fragments arranged by size: 

                4211    2473     300
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     308     343     358     409     582     721    1216 
   Size:        295      13      35      15      51     173     139     495

Cuts at:   1216    1462    1483    1918    2098    2200    2386    2395    2425 
   Size:        246      21     435     180     102     186       9      30

Cuts at:   2425    2659    2668    3019    3061    3448    3823    3841    5077 
   Size:        234       9     351      42     387     375      18    1236

Cuts at:   5077    5125    5368    5908    6046    6223    6290    6592    6984 
   Size:         48     243     540     138     177      67     302     392

  Fragments arranged by size: 

                1236     540     495     435     392     387     375     351
                 302     295     246     243     234     186     180     177
                 173     139     138     102      67      51      48      42
                  35      30      21      18      15      13       9       9
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1454    3077    3550    3707    4787    6062    6628    6772 
   Size:       1454    1623     473     157    1080    1275     566     144

Cuts at:   6772    6984 
   Size:        212

  Fragments arranged by size: 

                1623    1454    1275    1080     566     473     212     157
                 144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    3693    4380    4684    5068    6241    6257    6984 
   Size:        352    3341     687     304     384    1173      16     727

  Fragments arranged by size: 

                3341    1173     727     687     384     352     304      16
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1297    1396    1567    2887    5086    5206    5773    6243 
   Size:       1297      99     171    1320    2199     120     567     470

Cuts at:   6243    6984 
   Size:        741

  Fragments arranged by size: 

                2199    1320    1297     741     567     470     171     120
                  99
 
BsaAI yAC'GTr

Cuts at:      0    5172    6984 
   Size:       5172    1812
 
BsaBI GATnn'nnATC

Cuts at:      0     325    6984 
   Size:        325    6659
 
BsaHI Gr'CG_yC

Cuts at:      0      71    1007    1560    2282    2573    4745    4853    5480 
   Size:         71     936     553     722     291    2172     108     627

Cuts at:   5480    5682    6926    6984 
   Size:        202    1244      58

  Fragments arranged by size: 

                2172    1244     936     722     627     553     291     202
                 108      71      58
 
BsaWI w'CCGG_w

Cuts at:      0    1283    3620    6984 
   Size:       1283    2337    3364

  Fragments arranged by size: 

                3364    2337    1283
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     385     415     580     610     814     844    3034    3064 
   Size:        385      30     165      30     204      30    2190      30

Cuts at:   3064    3109    3139    4807    4837    5050    5080    5731    5761 
   Size:         45      30    1668      30     213      30     651      30

Cuts at:   5761    6814    6844    6984 
   Size:       1053      30     140

  Fragments arranged by size: 

                2190    1668    1053     651     385     213     204     165
                 140      45      30      30      30      30      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     739    1438    1873    2383    2392    2905    3133    4312 
   Size:        739     699     435     510       9     513     228    1179

Cuts at:   4312    4354    4361    4498    4681    5734    5796    5947    6274 
   Size:         42       7     137     183    1053      62     151     327

Cuts at:   6274    6292    6832    6835    6984 
   Size:         18     540       3     149

  Fragments arranged by size: 

                1179    1053     739     699     540     513     510     435
                 327     228     183     151     149     137      62      42
                  18       9       7       3
 
BseYI C'CCAG_C

Cuts at:      0      65     732    1879    3053    3168    3613    4115    4302 
   Size:         65     667    1147    1174     115     445     502     187

Cuts at:   4302    5541    5599    5690    6002    6179    6984 
   Size:       1239      58      91     312     177     805

  Fragments arranged by size: 

                1239    1174    1147     805     667     502     445     312
                 187     177     115      91      65      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1597    2677    3766    4054    5380    5575    6556    6984 
   Size:       1597    1080    1089     288    1326     195     981     428

  Fragments arranged by size: 

                1597    1326    1089    1080     981     428     288     195
            
 
BsiEI CG_ry'CG

Cuts at:      0    4721    4911    5492    6984 
   Size:       4721     190     581    1492

  Fragments arranged by size: 

                4721    1492     581     190
 
BsiHKAI G_wGCw'C

Cuts at:      0     284     707    1241    2018    2057    2402    2486    2870 
   Size:        284     423     534     777      39     345      84     384

Cuts at:   2870    3857    4141    4693    4814    5354    5513    5534    6629 
   Size:        987     284     552     121     540     159      21    1095

Cuts at:   6629    6853    6984 
   Size:        224     131

  Fragments arranged by size: 

                1095     987     777     552     540     534     423     384
                 345     284     284     224     159     131     121      84
                  39      21
 
BsmI GAATG_Cn'

Cuts at:      0     709    3456    5256    6984 
   Size:        709    2747    1800    1728

  Fragments arranged by size: 

                2747    1800    1728     709
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4462    6984 
   Size:       4462    2522
 
Bsp1286I G_dGCh'C

Cuts at:      0     284     403     707    1241    1440    2018    2057    2132 
   Size:        284     119     304     534     199     578      39      75

Cuts at:   2132    2340    2402    2486    2870    3338    3587    3857    4141 
   Size:        208      62      84     384     468     249     270     284

Cuts at:   4141    4682    4693    4814    4941    5354    5513    5534    6089 
   Size:        541      11     121     127     413     159      21     555

Cuts at:   6089    6452    6497    6581    6629    6853    6984 
   Size:        363      45      84      48     224     131

  Fragments arranged by size: 

                 578     555     541     534     468     413     384     363
                 304     284     284     270     249     224     208     199
                 159     131     127     121     119      84      84      75
                  62      48      45      39      21      11
 
BspEI T'CCGG_A

Cuts at:      0    3620    6984 
   Size:       3620    3364
 
BspHI T'CATG_A

Cuts at:      0    4547    5822    6984 
   Size:       4547    1275    1162

  Fragments arranged by size: 

                4547    1275    1162
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     251     947    1544    1703    2522    2953    3301    4154 
   Size:        251     696     597     159     819     431     348     853

Cuts at:   4154    4691    5402    5546    5946    6383    6984 
   Size:        537     711     144     400     437     601

  Fragments arranged by size: 

                 853     819     711     696     601     597     537     437
                 431     400     348     251     159     144
 
BsrBI CCG'CTC

Cuts at:      0     153     747    1428    2835    5235    5526    6009    6984 
   Size:        153     594     681    1407    2400     291     483     975

  Fragments arranged by size: 

                2400    1407     975     681     594     483     291     153
            
 
BsrDI GCAATG_nn'

Cuts at:      0    1301    6984 
   Size:       1301    5683
 
BsrFI r'CCGG_y

Cuts at:      0     644    2288    4073    6507    6984 
   Size:        644    1644    1785    2434     477

  Fragments arranged by size: 

                2434    1785    1644     644     477
 
BsrGI T'GTAC_A

Cuts at:      0     381    1139    2549    6984 
   Size:        381     758    1410    4435

  Fragments arranged by size: 

                4435    1410     758     381
 
BssHII G'CGCG_C

Cuts at:      0    2113    6984 
   Size:       2113    4871
 
BssSI C'ACGA_G

Cuts at:      0    1330    6984 
   Size:       1330    5654
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     332    1020    1545    2475    2529    3135    3147    3297 
   Size:        332     688     525     930      54     606      12     150

Cuts at:   3297    4536    5661    6534    6984 
   Size:       1239    1125     873     450

  Fragments arranged by size: 

                1239    1125     930     873     688     606     525     450
                 332     150      54      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2739    6669    6984 
   Size:       2739    3930     315

  Fragments arranged by size: 

                3930    2739     315
 
BstYI r'GATC_y

Cuts at:      0    1973    2084    3204    3617    4832    5423    5936    6984 
   Size:       1973     111    1120     413    1215     591     513    1048

  Fragments arranged by size: 

                1973    1215    1120    1048     591     513     413     111
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    4967    6984 
   Size:       4967    2017
 
BtgI C'CryG_G

Cuts at:      0    1046    2058    2385    4190    4594    6170    6443    6514 
   Size:       1046    1012     327    1805     404    1576     273      71

Cuts at:   6514    6522    6690    6804    6984 
   Size:          8     168     114     180

  Fragments arranged by size: 

                1805    1576    1046    1012     404     327     273     180
                 168     114      71       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1713    2264    2955    5286    6984 
   Size:       1713     551     691    2331    1698

  Fragments arranged by size: 

                2331    1713    1698     691     551
 
BtsI GCAGTG_nn'

Cuts at:      0     330     857    1585    1855    4564    5116    6984 
   Size:        330     527     728     270    2709     552    1868

  Fragments arranged by size: 

                2709    1868     728     552     527     330     270
 
ClaI AT'CG_AT

Cuts at:      0    3995    6984 
   Size:       3995    2989
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      35    2909    2944    6201    6236    6984 
   Size:         35    2874      35    3257      35     748

  Fragments arranged by size: 

                3257    2874     748      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2804    5508    6120    6984 
   Size:       2804    2704     612     864

  Fragments arranged by size: 

                2804    2704     864     612
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     487    1816    2650    2826    6984 
   Size:        487    1329     834     176    4158

  Fragments arranged by size: 

                4158    1329     834     487     176
 
EaeI y'GGCC_r

Cuts at:      0       8    2612    2751    3011    4718    4908    4928    5294 
   Size:          8    2604     139     260    1707     190      20     366

Cuts at:   5294    5378    5489    5573    6095    6173    6323    6984 
   Size:         84     111      84     522      78     150     661

  Fragments arranged by size: 

                2604    1707     661     522     366     260     190     150
                 139     111      84      84      78      20       8
 
EagI C'GGCC_G

Cuts at:      0    4718    4908    5489    6984 
   Size:       4718     190     581    1495

  Fragments arranged by size: 

                4718    1495     581     190
 
EarI CTCTTCn'nnn_

Cuts at:      0    1603    2426    4841    5033    5145    5183    6347    6805 
   Size:       1603     823    2415     192     112      38    1164     458

Cuts at:   6805    6949    6984 
   Size:        144      35

  Fragments arranged by size: 

                2415    1603    1164     823     458     192     144     112
                  38      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1899    2524    4657    5050    5722    6984 
   Size:       1899     625    2133     393     672    1262

  Fragments arranged by size: 

                2133    1899    1262     672     625     393
 
EcoICRI GAG'CTC

Cuts at:      0    1239    2055    2484    3855    4139    4691    4812    5352 
   Size:       1239     816     429    1371     284     552     121     540

Cuts at:   5352    5532    6984 
   Size:        180    1452

  Fragments arranged by size: 

                1452    1371    1239     816     552     540     429     284
                 180     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     308     343     358     409     582     694     721 
   Size:        295      13      35      15      51     173     112      27

Cuts at:    721    1014    1216    1462    1483    1795    1918    2098    2200 
   Size:        293     202     246      21     312     123     180     102

Cuts at:   2200    2203    2284    2386    2395    2425    2659    2668    3019 
   Size:          3      81     102       9      30     234       9     351

Cuts at:   3019    3061    3448    3560    3823    3841    4282    4888    4975 
   Size:         42     387     112     263      18     441     606      87

Cuts at:   4975    5044    5077    5125    5368    5709    5859    5908    6046 
   Size:         69      33      48     243     341     150      49     138

Cuts at:   6046    6223    6290    6592    6984 
   Size:        177      67     302     392

  Fragments arranged by size: 

                 606     441     392     387     351     341     312     302
                 295     293     263     246     243     234     202     180
                 177     173     150     138     123     112     112     102
                 102      87      81      69      67      51      49      48
                  42      35      33      30      27      21      18      15
                  13       9       9       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1453    2680    3280    4698    6457    6984 
   Size:       1453    1227     600    1418    1759     527

  Fragments arranged by size: 

                1759    1453    1418    1227     600     527
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125     240    1181    1446    2187    2337    2472    2643 
   Size:        125     115     941     265     741     150     135     171

Cuts at:   2643    2955    3132    3432    4227    4659    4944    6462    6577 
   Size:        312     177     300     795     432     285    1518     115

Cuts at:   6577    6602    6984 
   Size:         25     382

  Fragments arranged by size: 

                1518     941     795     741     432     382     312     300
                 285     265     177     171     150     135     125     115
                 115      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     630    1111    6780    6984 
   Size:        630     481    5669     204

  Fragments arranged by size: 

                5669     630     481     204
 
FspI TGC'GCA

Cuts at:      0     325    6984 
   Size:        325    6659
 
FspAI rTGC'GCAy

Cuts at:      0     325    6984 
   Size:        325    6659
 
HaeII r_GCGC'y

Cuts at:      0     729    1278    1329    1563    4190    4674    4856    5662 
   Size:        729     549      51     234    2627     484     182     806

Cuts at:   5662    5685    6192    6984 
   Size:         23     507     792

  Fragments arranged by size: 

                2627     806     792     729     549     507     484     234
                 182      51      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      88     120     775     807    1629    1661    3034    3066 
   Size:         88      32     655      32     822      32    1373      32

Cuts at:   3066    5731    5763    5791    5823    6255    6287    6322    6354 
   Size:       2665      32      28      32     432      32      35      32

Cuts at:   6354    6707    6739    6814    6846    6984 
   Size:        353      32      75      32     138

  Fragments arranged by size: 

                2665    1373     822     655     432     353     138      88
                  75      35      32      32      32      32      32      32
                  32      32      32      32      28
 
KasI G'GCGC_C

Cuts at:      0    1559    4852    5681    6984 
   Size:       1559    3293     829    1303

  Fragments arranged by size: 

                3293    1559    1303     829
 
KpnI G_GTAC'C

Cuts at:      0    1800    6984 
   Size:       1800    5184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1661    2810    4166    6620    6984 
   Size:       1661    1149    1356    2454     364

  Fragments arranged by size: 

                2454    1661    1356    1149     364
 
MscI TGG'CCA

Cuts at:      0    2753    4930    5575    6097    6984 
   Size:       2753    2177     645     522     887

  Fragments arranged by size: 

                2753    2177     887     645     522
 
MslI CAynn'nnrTG

Cuts at:      0     578     805    4861    5365    6407    6984 
   Size:        578     227    4056     504    1042     577

  Fragments arranged by size: 

                4056    1042     578     577     504     227
 
MspA1I CmG'CkG

Cuts at:      0      65     602     732     923    1020    1366    1879    2181 
   Size:         65     537     130     191      97     346     513     302

Cuts at:   2181    3060    3102    3186    3423    3750    4014    4203    4419 
   Size:        879      42      84     237     327     264     189     216

Cuts at:   4419    4596    4629    4717    4926    5373    5913    6002    6101 
   Size:        177      33      88     209     447     540      89      99

Cuts at:   6101    6179    6516    6984 
   Size:         78     337     468

  Fragments arranged by size: 

                 879     540     537     513     468     447     346     337
                 327     302     264     237     216     209     191     189
                 177     130      99      97      89      88      84      78
                  65      42      33
 
NaeI GCC'GGC

Cuts at:      0    6509    6984 
   Size:       6509     475
 
NarI GG'CG_CC

Cuts at:      0    1560    4853    5682    6984 
   Size:       1560    3293     829    1302

  Fragments arranged by size: 

                3293    1560    1302     829
 
NcoI C'CATG_G

Cuts at:      0    4190    6522    6690    6804    6984 
   Size:       4190    2332     168     114     180

  Fragments arranged by size: 

                4190    2332     180     168     114
 
NdeI CA'TA_TG

Cuts at:      0    5595    6984 
   Size:       5595    1389
 
NgoMIV G'CCGG_C

Cuts at:      0    6507    6984 
   Size:       6507     477
 
NheI G'CTAG_C

Cuts at:      0     927    6984 
   Size:        927    6057
 
NspI r_CATG'y

Cuts at:      0     261    1611    3267    3310    5067    5370    6021    6721 
   Size:        261    1350    1656      43    1757     303     651     700

Cuts at:   6721    6984 
   Size:        263

  Fragments arranged by size: 

                1757    1656    1350     700     651     303     263     261
                  43
 
PasI CC'CwG_GG

Cuts at:      0    2340    2418    3456    4941    5795    6675    6984 
   Size:       2340      78    1038    1485     854     880     309

  Fragments arranged by size: 

                2340    1485    1038     880     854     309      78
 
PciI A'CATG_T

Cuts at:      0    5063    6984 
   Size:       5063    1921
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     336     432    2496    2903    3426    4059    5541    5571 
   Size:        336      96    2064     407     523     633    1482      30

Cuts at:   5571    5697    6984 
   Size:        126    1287

  Fragments arranged by size: 

                2064    1482    1287     633     523     407     336     126
                  96      30
 
PfoI T'CCnGG_A

Cuts at:      0     127    1111    1601    1823    2090    2216    2363    2675 
   Size:        127     984     490     222     267     126     147     312

Cuts at:   2675    3509    4379    4388    5195    5345    5564    5749    5965 
   Size:        834     870       9     807     150     219     185     216

Cuts at:   5965    6604    6984 
   Size:        639     380

  Fragments arranged by size: 

                 984     870     834     807     639     490     380     312
                 267     222     219     216     185     150     147     127
                 126       9
 
PmlI CAC'GTG

Cuts at:      0    5172    6984 
   Size:       5172    1812
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     385     417    4360    4392    6351    6383    6984 
   Size:        385      32    3943      32    1959      32     601

  Fragments arranged by size: 

                3943    1959     601     385      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     125     240    1446    2187    2643    2955    4227    4659 
   Size:        125     115    1206     741     456     312    1272     432

Cuts at:   4659    6462    6602    6984 
   Size:       1803     140     382

  Fragments arranged by size: 

                1803    1272    1206     741     456     432     382     312
                 140     125     115
 
PshAI GACnn'nnGTC

Cuts at:      0    1006    2516    4237    6984 
   Size:       1006    1510    1721    2747

  Fragments arranged by size: 

                2747    1721    1510    1006
 
PspOMI G'GGCC_C

Cuts at:      0    2336    4937    6577    6984 
   Size:       2336    2601    1640     407

  Fragments arranged by size: 

                2601    2336    1640     407
 
PspXI vC'TCGA_Gb

Cuts at:      0     206    6984 
   Size:        206    6778
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2542    2574    5059    5091    6601    6633    6984 
   Size:       2542      32    2485      32    1510      32     351

  Fragments arranged by size: 

                2542    2485    1510     351      32      32      32
 
PstI C_TGCA'G

Cuts at:      0    1025    1541    1553    1592    2471    2534    3140    3152 
   Size:       1025     516      12      39     879      63     606      12

Cuts at:   3152    3191    3203    3302    3609    4094    4445    4571    4616 
   Size:         39      12      99     307     485     351     126      45

Cuts at:   4616    4688    4733    4959    5588    5912    5954    6380    6842 
   Size:         72      45     226     629     324      42     426     462

Cuts at:   6842    6984 
   Size:        142

  Fragments arranged by size: 

                1025     879     629     606     516     485     462     426
                 351     324     307     226     142     126      99      72
                  63      45      45      42      39      39      12      12
                  12
 
PvuII CAG'CTG

Cuts at:      0     602     732    1020    1366    1879    2181    3060    3186 
   Size:        602     130     288     346     513     302     879     126

Cuts at:   3186    3750    4014    4203    4419    4629    4926    5373    6179 
   Size:        564     264     189     216     210     297     447     806

Cuts at:   6179    6984 
   Size:        805

  Fragments arranged by size: 

                 879     806     805     602     564     513     447     346
                 302     297     288     264     216     210     189     130
                 126
 
SacI G_AGCT'C

Cuts at:      0    1241    2057    2486    3857    4141    4693    4814    5354 
   Size:       1241     816     429    1371     284     552     121     540

Cuts at:   5354    5534    6984 
   Size:        180    1450

  Fragments arranged by size: 

                1450    1371    1241     816     552     540     429     284
                 180     121
 
SacII CC_GC'GG

Cuts at:      0    4597    6517    6984 
   Size:       4597    1920     467

  Fragments arranged by size: 

                4597    1920     467
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1603    5033    6805    6984 
   Size:       1603    3430    1772     179

  Fragments arranged by size: 

                3430    1772    1603     179
 
SbfI CC_TGCA'GG

Cuts at:      0    1541    3152    3203    6984 
   Size:       1541    1611      51    3781

  Fragments arranged by size: 

                3781    1611    1541      51
 
ScaI AGT'ACT

Cuts at:      0    4765    6984 
   Size:       4765    2219
 
SexAI A'CCwGG_T

Cuts at:      0    2800    6984 
   Size:       2800    4184
 
SfcI C'TryA_G

Cuts at:      0    1021    1537    1549    1588    1699    2467    2530    3136 
   Size:       1021     516      12      39     111     768      63     606

Cuts at:   3136    3148    3187    3199    3298    3605    3826    4090    4441 
   Size:         12      39      12      99     307     221     264     351

Cuts at:   4441    4567    4612    4684    4729    4955    5584    5908    5950 
   Size:        126      45      72      45     226     629     324      42

Cuts at:   5950    6376    6838    6984 
   Size:        426     462     146

  Fragments arranged by size: 

                1021     768     629     606     516     462     426     351
                 324     307     264     226     221     146     126     111
                  99      72      63      45      45      42      39      39
                  12      12      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2335    3793    4936    6984 
   Size:       2335    1458    1143    2048

  Fragments arranged by size: 

                2335    2048    1458    1143
 
SfoI GGC'GCC

Cuts at:      0    1561    4854    5683    6984 
   Size:       1561    3293     829    1301

  Fragments arranged by size: 

                3293    1561    1301     829
 
SmaI CCC'GGG

Cuts at:      0    4220    5209    6344    6984 
   Size:       4220     989    1135     640

  Fragments arranged by size: 

                4220    1135     989     640
 
SmlI C'TyrA_G

Cuts at:      0     206     367    1570    3672    4359    4663    5083    6042 
   Size:        206     161    1203    2102     687     304     420     959

Cuts at:   6042    6220    6272    6984 
   Size:        178      52     712

  Fragments arranged by size: 

                2102    1203     959     712     687     420     304     206
                 178     161      52
 
SpeI A'CTAG_T

Cuts at:      0    1161    6984 
   Size:       1161    5823
 
SphI G_CATG'C

Cuts at:      0     261    3310    5370    6984 
   Size:        261    3049    2060    1614

  Fragments arranged by size: 

                3049    2060    1614     261
 
StuI AGG'CCT

Cuts at:      0     139    1027    2041    3193    3223    3796    4792    5926 
   Size:        139     888    1014    1152      30     573     996    1134

Cuts at:   5926    6984 
   Size:       1058

  Fragments arranged by size: 

                1152    1134    1058    1014     996     888     573     139
                  30
 
StyI C'CwwG_G

Cuts at:      0     140     311     524    1737    3384    3501    4190    4398 
   Size:        140     171     213    1213    1647     117     689     208

Cuts at:   4398    4838    6522    6690    6804    6984 
   Size:        440    1684     168     114     180

  Fragments arranged by size: 

                1684    1647    1213     689     440     213     208     180
                 171     168     140     117     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     177    1420    2771    3253    4467    5110    5533    6984 
   Size:        177    1243    1351     482    1214     643     423    1451

  Fragments arranged by size: 

                1451    1351    1243    1214     643     482     423     177
            
 
TatI w'GTAC_w

Cuts at:      0     381     917    1139    2549    4763    6617    6984 
   Size:        381     536     222    1410    2214    1854     367

  Fragments arranged by size: 

                2214    1854    1410     536     381     367     222
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     814     846    3109    3141    6104    6136    6984 
   Size:        814      32    2263      32    2963      32     848

  Fragments arranged by size: 

                2963    2263     848     814      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1345    2098    2337    2943    6984 
   Size:       1345     753     239     606    4041

  Fragments arranged by size: 

                4041    1345     753     606     239
 
XhoI C'TCGA_G

Cuts at:      0     206    1570    6042    6984 
   Size:        206    1364    4472     942

  Fragments arranged by size: 

                4472    1364     942     206
 
XmaI C'CCGG_G

Cuts at:      0    4218    5207    6342    6984 
   Size:       4218     989    1135     642

  Fragments arranged by size: 

                4218    1135     989     642
 
XmnI GAAnn'nnTTC

Cuts at:      0     161    1597    1924    2233    2435    4375    5254    6958 
   Size:        161    1436     327     309     202    1940     879    1704

Cuts at:   6958    6984 
   Size:         26

  Fragments arranged by size: 

                1940    1704    1436     879     327     309     202     161
                  26
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI    SacII     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AgeI     AhdI     AscI     AseI
    AsiSI     BaeI     BclI    BfrBI    BsiWI    BstBI   BstEII  BstZ17I
     DraI    EcoRI    EcoRV     FseI   HincII  HindIII     HpaI     MfeI
     MluI     NotI     NruI     NsiI     PacI     PmeI     PsiI     PvuI
    RsrII     SalI    SanDI     SgfI    SgrAI    SnaBI     SrfI     SspI
     SwaI  Tth111I     XbaI     ZraI
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