Restriction map for pF1KB4249
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        CpoI        
DraI        DraII       EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
HinP1I      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MroI        
MseI        MunI        MvnI        NarI        NciI        
NdeI        NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI          720  GGCGTC               G[GA]^CG[CT]C
Alw44I        916  GTGCAC               G^TGCAC
AlwI            3  GGATC                GGATC(4/5)
ApaLI         916  GTGCAC               G^TGCAC
Asp700I       981  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          601  CCCGAG               C^[CT]CG[GA]G
BglI          712  GCCATCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI           4  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         720  GGCGTC               G[GA]^CG[CT]C
BsmBI        1051  GAGACG               CGTCTC(1/5)
BsmFI         101  GTCCC                GGGAC(10/14)
BsoBI         601  CCCGAG               C^[CT]CG[GA]G
BspDI        1030  ATCGAT               AT^CGAT
BspMI         856  ACCTGC               ACCTGC(4/8)
BsrDI         261  CATTGC               GCAATG(2/0)
BssSI         310  CTCGTG               CACGAG(-5/-1)
BstAPI         68  GCAGCGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         182  CCAACTCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI           3  GGATCT               [GA]^GATC[CT]
ClaI         1030  ATCGAT               AT^CGAT
DraIII        897  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          552  GACCGTCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I         720  GGCGTC               G[GA]^CG[CT]C
HincII        928  GTCAAC               GT[CT]^[GA]AC
HindII        928  GTCAAC               GT[CT]^[GA]AC
HinfI        1043  GAGTC                G^A[ACGT]TC
MamI            4  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI            3  GGATCT               [GA]^GATC[CT]
MspA1I         51  CAGCTG               C[AC]G^C[GT]G
NaeI          860  GCCGGC               GCC^GGC
NcoI          332  CCATGG               C^CATGG
NgoMIV        860  GCCGGC               G^CCGGC
PleI         1043  GAGTC                GAGTC(4/5)
PvuII          51  CAGCTG               CAG^CTG
SacI          340  GAGCTC               GAGCT^C
StuI          983  AGGCCT               AGG^CCT
XcmI          182  CCAACTCCGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII           3  GGATCT               [GA]^GATC[CT]
XmnI          981  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          423  CCGC                 CCGC(-3/-1)
              811  GCGG                
AlwNI          69  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              901  CAGTGCCTG           
AvaII         420  GGACC                G^G[AT]CC
              590  GGTCC               
BanII         340  GAGCTC               G[GA]GC[CT]^C
             1015  GGGCTC              
BpmI         1038  CTCCAG               CTGGAG(16/14)
              865  CTGGAG              
BsaI          577  GAGACC               GGTCTC(1/5)
              642  GGTCTC              
BsgI          838  CTGCAC               GTGCAG(16/14)
              313  GTGCAG              
BsrFI         587  GCCGGT               [GA]^CCGG[CT]
              860  GCCGGC              
Cfr10I        587  GCCGGT               [GA]^CCGG[CT]
              860  GCCGGC              
DdeI           24  CTCAG                C^T[ACGT]AG
             1047  CTCAG               
DsaI          188  CCGTGG               C^C[GA][CT]GG
              332  CCATGG              
EcoT14I       332  CCATGG               C^C[AT][AT]GG
             1067  CCTTGG              
NspI          107  ACATGC               [GA]CATG^[CT]
              891  GCATGT              
ScaI          998  AGTACT               AGT^ACT
             1058  AGTACT              
SfcI          942  CTACAG               C^T[GA][CT]AG
              963  CTACAG              
StyI          332  CCATGG               C^C[AT][AT]GG
             1067  CCTTGG              
TaqI           17  TCGA                 T^CGA
             1031  TCGA                
TthHB8I        17  TCGA                 T^CGA
             1031  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AfaI        AluI        AspHI       BalI        BbrPI       
BbsI        BbvI        BmyI        BpuAI       BsaAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DpnII       EaeI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HgaI        HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      PmaCI       PmlI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        Tsp45I      TspRI       
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