Restriction map for pF1KB4249
 (Linear) (Six-base) MAPSORT of: KIBB4249.seq  Check: 3052  from: 1  to: 1086



KIBB4249.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     865    1086 
   Size:        865     221
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568    1086 
   Size:        568     518
 
AlwNI CAG_nnn'CTG

Cuts at:      0      74     906    1086 
   Size:         74     832     180

  Fragments arranged by size: 

                 832     180      74
 
ApaLI G'TGCA_C

Cuts at:      0     916    1086 
   Size:        916     170
 
AvaI C'yCGr_G

Cuts at:      0     601    1086 
   Size:        601     485
 
BanII G_rGCy'C

Cuts at:      0     344    1019    1086 
   Size:        344     675      67

  Fragments arranged by size: 

                 675     344      67
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     556     681     771    1086 
   Size:        556     125      90     315

  Fragments arranged by size: 

                 556     315     125      90
 
BglI GCCn_nnn'nGGC

Cuts at:      0     718    1086 
   Size:        718     368
 
Bme1580I G_kGCm'C

Cuts at:      0     920    1086 
   Size:        920     166
 
BmgBI CAC'GTC

Cuts at:      0     560    1086 
   Size:        560     526
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     286    1086 
   Size:        286     800
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     660    1086 
   Size:        660     426
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     886    1023    1086 
   Size:        886     137      63

  Fragments arranged by size: 

                 886     137      63
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     571     648    1086 
   Size:        571      77     438

  Fragments arranged by size: 

                 571     438      77
 
BsaAI yAC'GTr

Cuts at:      0     347     407     771     807    1086 
   Size:        347      60     364      36     279

  Fragments arranged by size: 

                 364     347     279      60      36
 
BsaBI GATnn'nnATC

Cuts at:      0       8    1086 
   Size:          8    1078
 
BsaHI Gr'CG_yC

Cuts at:      0     721    1086 
   Size:        721     365
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     124     154     814     844    1086 
   Size:        124      30     660      30     242

  Fragments arranged by size: 

                 660     242     124      30      30
 
BseYI C'CCAG_C

Cuts at:      0     457    1086 
   Size:        457     629
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     823    1086 
   Size:        334     489     263

  Fragments arranged by size: 

                 489     334     263
 
BsiHKAI G_wGCw'C

Cuts at:      0     344     608     872     920    1081    1086 
   Size:        344     264     264      48     161       5

  Fragments arranged by size: 

                 344     264     264     161      48       5
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1045    1086 
   Size:       1045      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     344     608     872     920    1019    1081    1086 
   Size:        344     264     264      48      99      62       5

  Fragments arranged by size: 

                 344     264     264      99      62      48       5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     865    1086 
   Size:        865     221
 
BsrDI GCAATG_nn'

Cuts at:      0     260    1086 
   Size:        260     826
 
BsrFI r'CCGG_y

Cuts at:      0     587     860    1086 
   Size:        587     273     226

  Fragments arranged by size: 

                 587     273     226
 
BssSI C'ACGA_G

Cuts at:      0     310    1086 
   Size:        310     776
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      74    1086 
   Size:         74    1012
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     189    1086 
   Size:        189     897
 
BstYI r'GATC_y

Cuts at:      0       3    1086 
   Size:          3    1083
 
BtgI C'CryG_G

Cuts at:      0     188     332    1086 
   Size:        188     144     754

  Fragments arranged by size: 

                 754     188     144
 
BtsI GCAGTG_nn'

Cuts at:      0     237     918    1086 
   Size:        237     681     168

  Fragments arranged by size: 

                 681     237     168
 
ClaI AT'CG_AT

Cuts at:      0    1031    1086 
   Size:       1031      55
 
DraIII CAC_nnn'GTG

Cuts at:      0     902    1086 
   Size:        902     184
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     558    1086 
   Size:        558     528
 
EaeI y'GGCC_r

Cuts at:      0      55     257     704    1004    1086 
   Size:         55     202     447     300      82

  Fragments arranged by size: 

                 447     300     202      82      55
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     388     883    1086 
   Size:         19     369     495     203

  Fragments arranged by size: 

                 495     369     203      19
 
EcoICRI GAG'CTC

Cuts at:      0     342    1086 
   Size:        342     744
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568     886    1023    1086 
   Size:        568     318     137      63

  Fragments arranged by size: 

                 568     318     137      63
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     777    1086 
   Size:        777     309
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     814     846    1086 
   Size:        814      32     240

  Fragments arranged by size: 

                 814     240      32
 
HincII GTy'rAC

Cuts at:      0     930    1086 
   Size:        930     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    1086 
   Size:        593     493
 
MscI TGG'CCA

Cuts at:      0      57     259     706    1086 
   Size:         57     202     447     380

  Fragments arranged by size: 

                 447     380     202      57
 
MslI CAynn'nnrTG

Cuts at:      0      82      94     172     409     415     499    1086 
   Size:         82      12      78     237       6      84     587

  Fragments arranged by size: 

                 587     237      84      82      78      12       6
 
MspA1I CmG'CkG

Cuts at:      0      53    1086 
   Size:         53    1033
 
NaeI GCC'GGC

Cuts at:      0     862    1086 
   Size:        862     224
 
NcoI C'CATG_G

Cuts at:      0     332    1086 
   Size:        332     754
 
NgoMIV G'CCGG_C

Cuts at:      0     860    1086 
   Size:        860     226
 
NspI r_CATG'y

Cuts at:      0     111     895    1086 
   Size:        111     784     191

  Fragments arranged by size: 

                 784     191     111
 
PmlI CAC'GTG

Cuts at:      0     347     407     807    1086 
   Size:        347      60     400     279

  Fragments arranged by size: 

                 400     347     279      60
 
PvuII CAG'CTG

Cuts at:      0      53    1086 
   Size:         53    1033
 
SacI G_AGCT'C

Cuts at:      0     344    1086 
   Size:        344     742
 
ScaI AGT'ACT

Cuts at:      0    1000    1060    1086 
   Size:       1000      60      26

  Fragments arranged by size: 

                1000      60      26
 
SfcI C'TryA_G

Cuts at:      0     942     963    1086 
   Size:        942      21     123

  Fragments arranged by size: 

                 942     123      21
 
StuI AGG'CCT

Cuts at:      0     985    1086 
   Size:        985     101
 
StyI C'CwwG_G

Cuts at:      0     332    1067    1086 
   Size:        332     735      19

  Fragments arranged by size: 

                 735     332      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189     288    1086 
   Size:        189      99     798

  Fragments arranged by size: 

                 798     189      99
 
TatI w'GTAC_w

Cuts at:      0     998    1058    1086 
   Size:        998      60      28

  Fragments arranged by size: 

                 998      60      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     189    1086 
   Size:        189     897
 
XmnI GAAnn'nnTTC

Cuts at:      0     985    1086 
   Size:        985     101
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI    ApaLI     AvaI    BanII     BbsI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI     BtsI
     ClaI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI     FalI
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PmlI    PvuII     SacI     ScaI     SfcI     StuI
     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI   Bpu10I    BpuEI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI    CspCI
     DraI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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