Restriction map for pF1KB4171
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     AspI        BbeI        BbvCI       
BclI        BfrI        BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmI        BspDI       BspEI       
BsrFI       BssHII      Bst1107I    BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HgaI        Hin1I       HindIII     
HpaI        KasI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SapI        SbfI        SfiI        SfoI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       2208  GGTACC               G^GTACC
AccI         1553  GTCGAC               GT^[AC][GT]AC
AccII          51  CGCG                 CG^CG
AcsI          295  AAATTC               [GA]^AATT[CT]
AhdI         1096  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1645  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       899  AGCGCT               AGC^GCT
ApoI          295  AAATTC               [GA]^AATT[CT]
Asp718I      2208  GGTACC               G^GTACC
AspEI        1096  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        2112  GAGCAC               G[AT]GC[AT]^C
AviII        1820  TGCGCA               TGC^GCA
BalI          987  TGGCCA               TGG^CCA
BbrPI         639  CACGTG               CAC^GTG
BcgI         2001  GCATTTTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         534  GTATCC               GTATCC(6/5)
BglII        1379  AGATCT               A^GATCT
BlpI         2707  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     2707  GCTAAGC              GC^T[ACGT]AGC
BsaAI         639  CACGTG               [CT]AC^GT[GA]
BsaBI         853  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      2112  GAGCAC               G[AT]GC[AT]^C
BssSI        1883  CTCGTG               CACGAG(-5/-1)
BstAPI       1870  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2237  TTCGAA               TT^CGAA
BstEII       3429  GGTCACC              G^GT[ACGT]ACC
BstPI        3429  GGTCACC              G^GT[ACGT]ACC
BstUI          51  CGCG                 CG^CG
BstXI        2213  CCACGGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1645  CCTCAGG              CC^T[ACGT]AGG
CelII        2707  GCTAAGC              GC^T[ACGT]AGC
DrdI         2536  GACCCAATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1096  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      899  AGCGCT               AGC^GCT
Eco81I       1645  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      3429  GGTCACC              G^GT[ACGT]ACC
FspI         1820  TGCGCA               TGC^GCA
HaeII         899  AGCGCT               [GA]GCGC^[CT]
KpnI         2208  GGTACC               GGTAC^C
KspI           50  CCGCGG               CCGC^GG
MamI          853  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         987  TGGCCA               TGG^CCA
MscI          987  TGGCCA               TGG^CCA
MvnI           51  CGCG                 CG^CG
NspV         2237  TTCGAA               TT^CGAA
PmaCI         639  CACGTG               CAC^GTG
PmlI          639  CACGTG               CAC^GTG
PpuMI         954  AGGTCCC              [GA]G^G[AT]CC[CT]
SacII          50  CCGCGG               CCGC^GG
SalI         1553  GTCGAC               G^TCGAC
ScaI           38  AGTACT               AGT^ACT
SfuI         2237  TTCGAA               TT^CGAA
SmaI          543  CCCGGG               CCC^GGG
StuI         2048  AGGCCT               AGG^CCT
XbaI          552  TCTAGA               T^CTAGA
XmaCI         543  CCCGGG               C^CCGGG
XmaI          543  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

ApaI         3092  GGGCCC               GGGCC^C
             3111  GGGCCC              
AvrII         700  CCTAGG               C^CTAGG
             2055  CCTAGG              
BamHI         644  GGATCC               G^GATCC
              722  GGATCC              
BlnI          700  CCTAGG               C^CTAGG
             2055  CCTAGG              
BsaI         2534  GAGACC               GGTCTC(1/5)
             2767  GAGACC              
BsgI          607  CTGCAC               GTGCAG(16/14)
              394  GTGCAG              
BsmBI        1165  GAGACG               CGTCTC(1/5)
             1717  GAGACG              
BspHI         581  TCATGA               T^CATGA
             1490  TCATGA              
BspLU11I      198  ACATGT               A^CATGT
             1829  ACATGT              
BspMI        1232  ACCTGC               ACCTGC(4/8)
              597  GCAGGT              
CfoI          900  GCGC                 GCG^C
             1821  GCGC                
HapII         544  CCGG                 C^CGG
             2832  CCGG                
HhaI          900  GCGC                 GCG^C
             1821  GCGC                
HinP1I        900  GCGC                 G^CGC
             1821  GCGC                
HpaII         544  CCGG                 C^CGG
             2832  CCGG                
MspI          544  CCGG                 C^CGG
             2832  CCGG                
NcoI         3039  CCATGG               C^CATGG
             3225  CCATGG              
PflMI        1593  CCATGTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3039  CCATGGCTTGG         
RcaI          581  TCATGA               T^CATGA
             1490  TCATGA              
SexAI        1535  ACCAGGT              A^CC[AT]GGT
             1706  ACCTGGT             
Van91I       1593  CCATGTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3039  CCATGGCTTGG         
XcmI         1127  CCATGACCGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2213  CCACGGCCTTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AlwNI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BsrBI       BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspBI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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