Restriction map for pF1KB4159
 (Circular) (Six-base) MAPSORT of: pF1KB4159.seq  Check: 216  from: 1  to: 3548



pF1KB4159  3548 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    586     586 
   Size:       3548
 
Acc65I G'GTAC_C

Cuts at:    564     564 
   Size:       3548
 
AclI AA'CG_TT

Cuts at:   3388    3388 
   Size:       3548
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    241    1373    1805    2552    2820     241 
   Size:       1132     432     747     268     969

  Fragments arranged by size: 

                1132     969     747     432     268
 
AflIII A'CryG_T

Cuts at:    900    2004    2981     900 
   Size:       1104     977    1467

  Fragments arranged by size: 

                1467    1104     977
 
AgeI A'CCGG_T

Cuts at:    173    1936     173 
   Size:       1763    1785
 
AlwNI CAG_nnn'CTG

Cuts at:    853    2420     853 
   Size:       1567    1981
 
ApaLI G'TGCA_C

Cuts at:   2318    2318 
   Size:       3548
 
ApoI r'AATT_y

Cuts at:    457     552    2901    3157     457 
   Size:         95    2349     256     848

  Fragments arranged by size: 

                2349     848     256      95
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3548
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3548
 
AvaI C'yCGr_G

Cuts at:    568     568 
   Size:       3548
 
BamHI G'GATC_C

Cuts at:    573     573 
   Size:       3548
 
BanI G'GyrC_C

Cuts at:    564     978    1226    1261     564 
   Size:        414     248      35    2851

  Fragments arranged by size: 

                2851     414     248      35
 
BanII G_rGCy'C

Cuts at:    262     562    1592     262 
   Size:        300    1030    2218

  Fragments arranged by size: 

                2218    1030     300
 
BbeI G_GCGC'C

Cuts at:    982    1230     982 
   Size:        248    3300
 
BbsI GAAGACnn'nnnn_

Cuts at:    218     218 
   Size:       3548
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3514
 
BciVI GTATCCnnnnn_n'

Cuts at:   1446    2207    3548    1446 
   Size:        761    1341    1446

  Fragments arranged by size: 

                1446    1341     761
 
BclI T'GATC_A

Cuts at:   1068    1068 
   Size:       3548
 
BglII A'GATC_T

Cuts at:   1063    3100    1063 
   Size:       2037    1511
 
BlpI GC'TnA_GC

Cuts at:    660     660 
   Size:       3548
 
Bme1580I G_kGCm'C

Cuts at:   1173    1266    2322    1173 
   Size:         93    1056    2399

  Fragments arranged by size: 

                2399    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    760     841    1176    3320     760 
   Size:         81     335    2144     988

  Fragments arranged by size: 

                2144     988     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    276    1915     276 
   Size:       1639    1909
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    724    1603    2095    2393     724 
   Size:        879     492     298    1879

  Fragments arranged by size: 

                1879     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3252    3252 
   Size:       3548
 
BsaAI yAC'GTr

Cuts at:    901     901 
   Size:       3548
 
BsaBI GATnn'nnATC

Cuts at:      6    1062    3099       6 
   Size:       1056    2037     455

  Fragments arranged by size: 

                2037    1056     455
 
BsaHI Gr'CG_yC

Cuts at:    979    1227    1929    3425     979 
   Size:        248     702    1496    1102

  Fragments arranged by size: 

                1496    1102     702     248
 
BsaWI w'CCGG_w

Cuts at:    173     737    1258    1936    2210    2357    2768     173 
   Size:        564     521     678     274     147     411     953

  Fragments arranged by size: 

                 953     678     564     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    973    1731    2308    2804     973 
   Size:        758     577     496    1717

  Fragments arranged by size: 

                1717     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1090    1136    2344     104 
   Size:        986      46    1208    1308

  Fragments arranged by size: 

                1308    1208     986      46
 
BsiHKAI G_wGCw'C

Cuts at:    562    1340    1530    2322     562 
   Size:        778     190     792    1788

  Fragments arranged by size: 

                1788     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    262     562    1173    1266    1340    1530    1592    2322     262 
   Size:        300     611      93      74     190      62     730    1488

  Fragments arranged by size: 

                1488     730     611     300     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2768    2768 
   Size:       3548
 
BspHI T'CATG_A

Cuts at:   2724    2724 
   Size:       3548
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    598     812    1114    1495     598 
   Size:        214     302     381    2651

  Fragments arranged by size: 

                2651     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1840    1894    3378    3543      50 
   Size:       1790      54    1484     165      55

  Fragments arranged by size: 

                1790    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1460    1460 
   Size:       3548
 
BsrFI r'CCGG_y

Cuts at:    173    1546    1727    1936     173 
   Size:       1373     181     209    1785

  Fragments arranged by size: 

                1785    1373     209     181
 
BsrGI T'GTAC_A

Cuts at:    269     269 
   Size:       3548
 
BssSI C'ACGA_G

Cuts at:   1819    2177    1819 
   Size:        358    3190
 
BstAPI GCAn_nnn'nTGC

Cuts at:    925     925 
   Size:       3548
 
BstBI TT'CG_AA

Cuts at:   1909    1909 
   Size:       3548
 
BstYI r'GATC_y

Cuts at:      1     573    1040    1063    1398    1644    2656    2742    2754 
   Size:        572     467      23     335     246    1012      86      12

Cuts at:   2754    3100       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     572     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1498    1679    1842    1498 
   Size:        181     163    3204

  Fragments arranged by size: 

                3204     181     163
 
BtsI GCAGTG_nn'

Cuts at:    791     791 
   Size:       3548
 
DraI TTT'AAA

Cuts at:    547     547 
   Size:       3548
 
DrdI GACnn_nn'nnGTC

Cuts at:   1254    2112    1254 
   Size:        858    2690
 
EaeI y'GGCC_r

Cuts at:   1133    1307    1698    1725    3070    1133 
   Size:        174     391      27    1345    1611

  Fragments arranged by size: 

                1611    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1133    1133 
   Size:       3548
 
EarI CTCTTCn'nnn_

Cuts at:   1571    1781    1571 
   Size:        210    3338
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1756    2066    2212    3358    1756 
   Size:        310     146    1146    1946

  Fragments arranged by size: 

                1946    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    560     560 
   Size:       3548
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    241    1373    1805    2552    2820     241 
   Size:       1132     432     747     268     969

  Fragments arranged by size: 

                1132     969     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    687     687 
   Size:       3548
 
EcoRI G'AATT_C

Cuts at:    552     552 
   Size:       3548
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    301    1567     301 
   Size:       1266    2282
 
FspI TGC'GCA

Cuts at:   1329    1329 
   Size:       3548
 
HaeII r_GCGC'y

Cuts at:    982    1230    2252    3231     982 
   Size:        248    1022     979    1299

  Fragments arranged by size: 

                1299    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1390    1420    1422    1452    1390 
   Size:         30       2      30    3486

  Fragments arranged by size: 

                3486      30      30       2
 
HincII GTy'rAC

Cuts at:    587     587 
   Size:       3548
 
KasI G'GCGC_C

Cuts at:    978    1226     978 
   Size:        248    3300
 
KpnI G_GTAC'C

Cuts at:    568     568 
   Size:       3548
 
MluI A'CGCG_T

Cuts at:   2981    2981 
   Size:       3548
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    429    2219    2403     429 
   Size:       1790     184    1574

  Fragments arranged by size: 

                1790    1574     184
 
MscI TGG'CCA

Cuts at:   1309    1309 
   Size:       3548
 
MslI CAynn'nnrTG

Cuts at:   1537    1664    1537 
   Size:        127    3421
 
MspA1I CmG'CkG

Cuts at:    659     973    1333    2346    2591     659 
   Size:        314     360    1013     245    1616

  Fragments arranged by size: 

                1616    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1729    1729 
   Size:       3548
 
NarI GG'CG_CC

Cuts at:    979    1227     979 
   Size:        248    3300
 
NgoMIV G'CCGG_C

Cuts at:   1727    1727 
   Size:       3548
 
NspI r_CATG'y

Cuts at:    422     601    1101    2008     422 
   Size:        179     500     907    1962

  Fragments arranged by size: 

                1962     907     500     179
 
PciI A'CATG_T

Cuts at:   2004    2004 
   Size:       3548
 
PmeI GTTT'AAAC

Cuts at:    547     547 
   Size:       3548
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2687    2719    2687 
   Size:         32    3516
 
PstI C_TGCA'G

Cuts at:    595    1280     595 
   Size:        685    2863
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3548
 
PvuII CAG'CTG

Cuts at:    973    1333     973 
   Size:        360    3188
 
RsrII CG'GwC_CG

Cuts at:   1743    1743 
   Size:       3548
 
SacI G_AGCT'C

Cuts at:    562     562 
   Size:       3548
 
SalI G'TCGA_C

Cuts at:    585     585 
   Size:       3548
 
SapI GCTCTTCn'nnn_

Cuts at:   1571    1781    1571 
   Size:        210    3338
 
SbfI CC_TGCA'GG

Cuts at:    595     595 
   Size:       3548
 
ScaI AGT'ACT

Cuts at:   3065    3065 
   Size:       3548
 
SfcI C'TryA_G

Cuts at:     33     345     591    1276    2269    2460      33 
   Size:        312     246     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     312     246     191
 
SfoI GGC'GCC

Cuts at:    980    1228     980 
   Size:        248    3300
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3548
 
SmaI CCC'GGG

Cuts at:    570     570 
   Size:       3548
 
SmlI C'TyrA_G

Cuts at:    703    1618    2110    2372     703 
   Size:        915     492     262    1879

  Fragments arranged by size: 

                1879     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3548
 
SphI G_CATG'C

Cuts at:    601    1101     601 
   Size:        500    3048
 
StyI C'CwwG_G

Cuts at:    386     682    1036     386 
   Size:        296     354    2898

  Fragments arranged by size: 

                2898     354     296
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1131    1264    1723    1932    1131 
   Size:        133     459     209    2747

  Fragments arranged by size: 

                2747     459     209     133
 
TatI w'GTAC_w

Cuts at:    269    3063     269 
   Size:       2794     754
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3065    3097    3065 
   Size:         32    3516
 
Tth111I GACn'n_nGTC

Cuts at:   1345    1345 
   Size:       3548
 
XbaI T'CTAG_A

Cuts at:    579     579 
   Size:       3548
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1043    1043 
   Size:       3548
 
XmaI C'CCGG_G

Cuts at:    568     568 
   Size:       3548
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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