Restriction map for pF1KB4141
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaBI       BsiWI       BsmBI       BsmI        BspDI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoRV       EcoT22I     FbaI        
FseI        FspI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfuI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        TfiI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII         168  CGCG                 CG^CG
AccIII        501  TCCGGA               T^CCGGA
AcsI         1251  GAATTC               [GA]^AATT[CT]
Alw44I        374  GTGCAC               G^TGCAC
Aor51HI       986  AGCGCT               AGC^GCT
ApaLI         374  GTGCAC               G^TGCAC
ApoI         1251  GAATTC               [GA]^AATT[CT]
AspHI         374  GTGCAC               G[AT]GC[AT]^C
AspI           56  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          616  GGCGCC               GGCGC^C
BfaI          331  CTAG                 C^TAG
BsaAI         226  TACGTG               [CT]AC^GT[GA]
BseAI         501  TCCGGA               T^CCGGA
BsgI         2023  GTGCAG               GTGCAG(16/14)
BsiEI        2168  CGGTCG               CG[GA][CT]^CG
BsiHKAI       374  GTGCAC               G[AT]GC[AT]^C
BspEI         501  TCCGGA               T^CCGGA
BspHI        2048  TCATGA               T^CATGA
BsrFI         613  ACCGGC               [GA]^CCGG[CT]
BstAPI        338  GCATGGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1968  GGTCACC              G^GT[ACGT]ACC
BstPI        1968  GGTCACC              G^GT[ACGT]ACC
BstUI         168  CGCG                 CG^CG
Cfr10I        613  ACCGGC               [GA]^CCGG[CT]
DraI         1320  TTTAAA               TTT^AAA
DraIII        379  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      986  AGCGCT               AGC^GCT
EcoO65I      1968  GGTCACC              G^GT[ACGT]ACC
EcoRI        1251  GAATTC               G^AATTC
HincII       1009  GTTGAC               GT[CT]^[GA]AC
HindII       1009  GTTGAC               GT[CT]^[GA]AC
HindIII        13  AAGCTT               A^AGCTT
KasI          616  GGCGCC               G^GCGCC
MaeI          331  CTAG                 C^TAG
MaeII         227  ACGT                 A^CGT
MroI          501  TCCGGA               T^CCGGA
MseI         1321  TTAA                 T^TAA
MvnI          168  CGCG                 CG^CG
NarI          616  GGCGCC               GG^CGCC
PflFI          56  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         681  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2048  TCATGA               T^CATGA
SexAI          57  ACCAGGT              A^CC[AT]GGT
SfiI          929  GGCCGAAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          616  GGCGCC               GGC^GCC
SgrAI         612  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         1462  CCCGGG               CCC^GGG
SphI         1421  GCATGC               GCATG^C
Tru9I        1321  TTAA                 T^TAA
Tth111I        56  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        681  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1462  CCCGGG               C^CCGGG
XmaI         1462  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BanI          616  GGCGCC               G^G[CT][GA]CC
             1867  GGCACC              
BglI          733  GCCAGTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              930  GCCGAAGTGGC         
BlpI         1833  GCTGAGC              GC^T[ACGT]AGC
             2065  GCTGAGC             
Bpu1102I     1833  GCTGAGC              GC^T[ACGT]AGC
             2065  GCTGAGC             
BsaWI           8  ACCGGA               [AT]^CCGG[AT]
              501  TCCGGA              
Bsp1407I     1655  TGTACA               T^GTACA
             2240  TGTACA              
BspMI        1897  ACCTGC               ACCTGC(4/8)
              948  GCAGGT              
BsrBI        1821  CCGCTC               CCGCTC(-3/-3)
               30  GAGCGG              
BsrDI        2061  CATTGC               GCAATG(2/0)
              956  GCAATG              
BsrGI        1655  TGTACA               T^GTACA
             2240  TGTACA              
BsrI          734  CCAGT                ACTGG(1/-1)
             1539  CCAGT               
BstXI        1121  CCAATTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1871  CCAGCCTGGTGG        
BstYI        1730  AGATCC               [GA]^GATC[CT]
             2177  AGATCC              
CelII        1833  GCTGAGC              GC^T[ACGT]AGC
             2065  GCTGAGC             
EarI         1291  CTCTTC               CTCTTC(1/4)
              922  GAAGAG              
Ksp632I      1291  CTCTTC               CTCTTC(1/4)
              922  GAAGAG              
MaeIII        551  GTCAC                ^GT[ACGT]AC
             1969  GTCAC               
MflI         1730  AGATCC               [GA]^GATC[CT]
             2177  AGATCC              
NspI           35  GCATGT               [GA]CATG^[CT]
             1421  GCATGC              
SspBI        1655  TGTACA               T^GTACA
             2240  TGTACA              
StuI          788  AGGCCT               AGG^CCT
             1553  AGGCCT              
Tsp45I        551  GTCAC                ^GT[GC]AC
             1969  GTCAC               
XhoII        1730  AGATCC               [GA]^GATC[CT]
             2177  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AvaI        AvaII       
BalI        BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BssKI       BssSI       
BstF5I      BstNI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp509I     TspRI       
TthHB8I     XcmI        
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