Restriction map for pF1KB4121
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BlnI        BpuAI       
BsaAI       BsaI        BsgI        BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
Cfr10I      ClaI        CpoI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FbaI        
FseI        FspI        HaeII       HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MunI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
Van91I      XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII          63  GACGTC               GACGT^C
AccI         1026  GTCTAC               GT^[AC][GT]AC
AccIII        638  TCCGGA               T^CCGGA
AcyI           63  GACGTC               G[GA]^CG[CT]C
AflIII        419  ACGCGT               A^C[GA][CT]GT
AocI          354  CCTGAGG              CC^T[ACGT]AGG
AseI          562  ATTAAT               AT^TAAT
AsnI          562  ATTAAT               AT^TAAT
AvaII         659  GGTCC                G^G[AT]CC
BfaI          325  CTAG                 C^TAG
BglI          859  GCCAACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          87  AGATCT               A^GATCT
BlpI         1147  GCTCAGC              GC^T[ACGT]AGC
BpmI         1315  CTCCAG               CTGGAG(16/14)
Bpu1102I     1147  GCTCAGC              GC^T[ACGT]AGC
BsaBI         895  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          63  GACGTC               G[GA]^CG[CT]C
BsaWI         638  TCCGGA               [AT]^CCGG[AT]
BseAI         638  TCCGGA               T^CCGGA
BsmBI          65  CGTCTC               CGTCTC(1/5)
BspEI         638  TCCGGA               T^CCGGA
BsrDI        1120  CATTGC               GCAATG(2/0)
BssSI         411  CACGAG               CACGAG(-5/-1)
BstBI        1207  TTCGAA               TT^CGAA
Bsu36I        354  CCTGAGG              CC^T[ACGT]AGG
CelII        1147  GCTCAGC              GC^T[ACGT]AGC
CfoI          293  GCGC                 GCG^C
DraII         658  AGGTCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        354  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      658  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       896  ATGCAT               ATGCA^T
FauI          951  GCGGG                CCCGC(4/6)
HapII         639  CCGG                 C^CGG
HgaI         1584  GCGTC                GACGC(5/10)
HhaI          293  GCGC                 GCG^C
Hin1I          63  GACGTC               G[GA]^CG[CT]C
HinP1I        293  GCGC                 G^CGC
HindIII      1741  AAGCTT               A^AGCTT
HpaII         639  CCGG                 C^CGG
MaeI          325  CTAG                 C^TAG
MamI          895  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          419  ACGCGT               A^CGCGT
MroI          638  TCCGGA               T^CCGGA
MspA1I       1150  CAGCTG               C[AC]G^C[GT]G
MspI          639  CCGG                 C^CGG
NdeI          153  CATATG               CA^TATG
NsiI          896  ATGCAT               ATGCA^T
NspV         1207  TTCGAA               TT^CGAA
PpuMI         658  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI         562  ATTAAT               AT^TAAT
PstI         1720  CTGCAG               CTGCA^G
PvuII        1150  CAGCTG               CAG^CTG
SfuI         1207  TTCGAA               TT^CGAA
SspI          937  AATATT               AAT^ATT
XbaI          324  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AciI          951  GCGG                 CCGC(-3/-1)
             1707  GCGG                
AfaI         1219  GTAC                 GT^AC
             1427  GTAC                
AspHI         577  GAGCTC               G[AT]GC[AT]^C
             1675  GAGCTC              
BalI          857  TGGCCA               TGG^CCA
             1163  TGGCCA              
BanII         577  GAGCTC               G[GA]GC[CT]^C
             1675  GAGCTC              
BbvI         1662  GCAGC                GCAGC(8/12)
             1722  GCAGC               
BmyI          577  GAGCTC               G[AGT]GC[ACT]^C
             1675  GAGCTC              
BseRI         305  GAGGAG               GAGGAG(10/8)
              875  GAGGAG              
BsiHKAI       577  GAGCTC               G[AT]GC[AT]^C
             1675  GAGCTC              
BsmAI         796  GAGAC                GTCTC(1/5)
               66  GTCTC               
BsmFI        1136  GGGAC                GGGAC(10/14)
              660  GTCCC               
Bsp1286I      577  GAGCTC               G[AGT]GC[ACT]^C
             1675  GAGCTC              
BstAPI        525  GCAGGGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1779  GCAAATGATGC         
BstYI          87  AGATCT               [GA]^GATC[CT]
              404  AGATCC              
Cac8I         530  GCTTGC               GC[ACGT]^[ACGT]GC
             1725  GCTCGC              
Cfr13I        169  GGCCC                G^G[ACGT]CC
              659  GGTCC               
DraI          459  TTTAAA               TTT^AAA
              960  TTTAAA              
EaeI          857  TGGCCA               [CT]^GGCC[GA]
             1163  TGGCCA              
EcoRI         671  GAATTC               G^AATTC
             1369  GAATTC              
EcoT14I        38  CCTTGG               C^C[AT][AT]GG
             1160  CCTTGG              
MaeII          64  ACGT                 A^CGT
              479  ACGT                
MflI           87  AGATCT               [GA]^GATC[CT]
              404  AGATCC              
MluNI         857  TGGCCA               TGG^CCA
             1163  TGGCCA              
MscI          857  TGGCCA               TGG^CCA
             1163  TGGCCA              
RsaI         1219  GTAC                 GT^AC
             1427  GTAC                
SacI          577  GAGCTC               GAGCT^C
             1675  GAGCTC              
Sau96I        169  GGCCC                G^G[ACGT]CC
              659  GGTCC               
StyI           38  CCTTGG               C^C[AT][AT]GG
             1160  CCTTGG              
TseI         1662  GCAGC                G^C[AT]GC
             1722  GCAGC               
XcmI          676  CCAGTGCTGTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1166  CCATGCTGATGTTGG     
XhoII          87  AGATCT               [GA]^GATC[CT]
              404  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccII       AcsI        AluI        AlwI        ApoI        
AvaI        BsaJI       BsiYI       BslI        BsoBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PaeR7I      PleI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoI        
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