Restriction map for pF1KB4121
 (Linear) (Six-base) MAPSORT of: KIBB4121.seq  Check: 328  from: 1  to: 1809



KIBB4121.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AatII G_ACGT'C

Cuts at:      0      67    1809 
   Size:         67    1742
 
AccI GT'mk_AC

Cuts at:      0    1027    1809 
   Size:       1027     782
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1079    1809 
   Size:       1079     730
 
AflIII A'CryG_T

Cuts at:      0     419    1809 
   Size:        419    1390
 
ApoI r'AATT_y

Cuts at:      0     312     671     809    1249    1369    1522    1809 
   Size:        312     359     138     440     120     153     287

  Fragments arranged by size: 

                 440     359     312     287     153     138     120
 
AseI AT'TA_AT

Cuts at:      0     563    1809 
   Size:        563    1246
 
AvaI C'yCGr_G

Cuts at:      0      68     270    1454    1809 
   Size:         68     202    1184     355

  Fragments arranged by size: 

                1184     355     202      68
 
BanII G_rGCy'C

Cuts at:      0     581    1679    1809 
   Size:        581    1098     130

  Fragments arranged by size: 

                1098     581     130
 
BfrBI ATG'CAT

Cuts at:      0     898    1809 
   Size:        898     911
 
BglI GCCn_nnn'nGGC

Cuts at:      0     865    1809 
   Size:        865     944
 
BglII A'GATC_T

Cuts at:      0      87    1809 
   Size:         87    1722
 
BlpI GC'TnA_GC

Cuts at:      0    1148    1809 
   Size:       1148     661
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      83    1809 
   Size:         83    1726
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1300    1809 
   Size:       1300     509
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1732    1809 
   Size:       1732      77
 
BsaBI GATnn'nnATC

Cuts at:      0     899    1809 
   Size:        899     910
 
BsaHI Gr'CG_yC

Cuts at:      0      64    1809 
   Size:         64    1745
 
BsaWI w'CCGG_w

Cuts at:      0     638    1809 
   Size:        638    1171
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      34      64    1809 
   Size:         34      30    1745

  Fragments arranged by size: 

                1745      34      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     320     890    1809 
   Size:        320     570     919

  Fragments arranged by size: 

                 919     570     320
 
BseYI C'CCAG_C

Cuts at:      0     841    1809 
   Size:        841     968
 
BsiHKAI G_wGCw'C

Cuts at:      0     581    1679    1809 
   Size:        581    1098     130

  Fragments arranged by size: 

                1098     581     130
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      71    1809 
   Size:         71    1738
 
Bsp1286I G_dGCh'C

Cuts at:      0     581    1679    1809 
   Size:        581    1098     130

  Fragments arranged by size: 

                1098     581     130
 
BspEI T'CCGG_A

Cuts at:      0     638    1809 
   Size:        638    1171
 
BsrDI GCAATG_nn'

Cuts at:      0    1119    1809 
   Size:       1119     690
 
BssSI C'ACGA_G

Cuts at:      0     411    1809 
   Size:        411    1398
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     531    1785    1809 
   Size:        531    1254      24

  Fragments arranged by size: 

                1254     531      24
 
BstBI TT'CG_AA

Cuts at:      0    1208    1809 
   Size:       1208     601
 
BstYI r'GATC_y

Cuts at:      0      87     404    1809 
   Size:         87     317    1405

  Fragments arranged by size: 

                1405     317      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     355    1809 
   Size:        355    1454
 
BtsI GCAGTG_nn'

Cuts at:      0     860    1131    1809 
   Size:        860     271     678

  Fragments arranged by size: 

                 860     678     271
 
DraI TTT'AAA

Cuts at:      0     461     962    1809 
   Size:        461     501     847

  Fragments arranged by size: 

                 847     501     461
 
EaeI y'GGCC_r

Cuts at:      0     857    1163    1809 
   Size:        857     306     646

  Fragments arranged by size: 

                 857     646     306
 
EarI CTCTTCn'nnn_

Cuts at:      0     177     384    1096    1809 
   Size:        177     207     712     713

  Fragments arranged by size: 

                 713     712     207     177
 
EcoICRI GAG'CTC

Cuts at:      0     579    1677    1809 
   Size:        579    1098     132

  Fragments arranged by size: 

                1098     579     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1079    1300    1809 
   Size:       1079     221     509

  Fragments arranged by size: 

                1079     509     221
 
EcoO109I rG'GnC_Cy

Cuts at:      0     659    1809 
   Size:        659    1150
 
EcoRI G'AATT_C

Cuts at:      0     671    1369    1809 
   Size:        671     698     440

  Fragments arranged by size: 

                 698     671     440
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      62      66      94     176     208    1809 
   Size:         34      28       4      28      82      32    1601

  Fragments arranged by size: 

                1601      82      34      32      28      28       4
 
HindIII A'AGCT_T

Cuts at:      0    1741    1809 
   Size:       1741      68
 
MluI A'CGCG_T

Cuts at:      0     419    1809 
   Size:        419    1390
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1157    1809 
   Size:       1157     652
 
MscI TGG'CCA

Cuts at:      0     859    1165    1809 
   Size:        859     306     644

  Fragments arranged by size: 

                 859     644     306
 
MslI CAynn'nnrTG

Cuts at:      0    1124    1171    1289    1809 
   Size:       1124      47     118     520

  Fragments arranged by size: 

                1124     520     118      47
 
MspA1I CmG'CkG

Cuts at:      0    1152    1809 
   Size:       1152     657
 
NdeI CA'TA_TG

Cuts at:      0     154    1809 
   Size:        154    1655
 
NsiI A_TGCA'T

Cuts at:      0     900    1809 
   Size:        900     909
 
PfoI T'CCnGG_A

Cuts at:      0     114    1809 
   Size:        114    1695
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1709    1741    1809 
   Size:       1709      32      68

  Fragments arranged by size: 

                1709      68      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     659    1809 
   Size:        659    1150
 
PsiI TTA'TAA

Cuts at:      0    1393    1809 
   Size:       1393     416
 
PstI C_TGCA'G

Cuts at:      0    1724    1809 
   Size:       1724      85
 
PvuII CAG'CTG

Cuts at:      0    1152    1809 
   Size:       1152     657
 
SacI G_AGCT'C

Cuts at:      0     581    1679    1809 
   Size:        581    1098     130

  Fragments arranged by size: 

                1098     581     130
 
SfcI C'TryA_G

Cuts at:      0    1188    1358    1720    1809 
   Size:       1188     170     362      89

  Fragments arranged by size: 

                1188     362     170      89
 
SmlI C'TyrA_G

Cuts at:      0      68     270    1454    1711    1809 
   Size:         68     202    1184     257      98

  Fragments arranged by size: 

                1184     257     202      98      68
 
SspI AAT'ATT

Cuts at:      0     939    1809 
   Size:        939     870
 
StyI C'CwwG_G

Cuts at:      0      38    1160    1809 
   Size:         38    1122     649

  Fragments arranged by size: 

                1122     649      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250    1590    1809 
   Size:        250    1340     219

  Fragments arranged by size: 

                1340     250     219
 
XbaI T'CTAG_A

Cuts at:      0     324    1809 
   Size:        324    1485
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     683    1173    1809 
   Size:        683     490     636

  Fragments arranged by size: 

                 683     636     490
 
XhoI C'TCGA_G

Cuts at:      0      68     270    1454    1809 
   Size:         68     202    1184     355

  Fragments arranged by size: 

                1184     355     202      68
 
ZraI GAC'GTC

Cuts at:      0      65    1809 
   Size:         65    1744
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     ApoI     AseI     AvaI    BanII
    BfrBI     BglI    BglII     BlpI     BplI     BpmI    BpuEI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BsrDI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtsI
     DraI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I
  HindIII     MluI     MmeI     MscI     MslI   MspA1I     NdeI     NsiI
     PfoI     PpiI    PpuMI     PsiI     PstI    PvuII     SacI     SfcI
     SmlI     SspI     StyI    TaqII     XbaI     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI
     BsgI    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI
     TstI  Tth111I     XmaI     XmnI
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