Restriction map for pF1KB4102
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvaI        AvrII       
BamHI       BbrPI       BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BseAI       
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MamI        MfeI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        ScaI        SexAI       SfuI        
SgrAI       SmaI        SnaBI       SpeI        SspI        
SwaI        TfiI        Tru9I       XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcsI         2116  GAATTC               [GA]^AATT[CT]
AocI         1091  CCTCAGG              CC^T[ACGT]AGG
ApoI         2116  GAATTC               [GA]^AATT[CT]
AspHI        1549  GAGCAC               G[AT]GC[AT]^C
AspI          306  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII          34  GAGCCC               G[GA]GC[CT]^C
BbeI         1372  GGCGCC               GGCGC^C
BbsI         1510  GTCTTC               GAAGAC(2/6)
BglII        1730  AGATCT               A^GATCT
BpuAI        1510  GTCTTC               GAAGAC(2/6)
BsaAI         139  TACGTG               [CT]AC^GT[GA]
BsaWI         296  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1549  GAGCAC               G[AT]GC[AT]^C
BsmBI         130  GAGACG               CGTCTC(1/5)
Bsp1407I      219  TGTACA               T^GTACA
BsrGI         219  TGTACA               T^GTACA
BstXI          38  CCAACGCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1091  CCTCAGG              CC^T[ACGT]AGG
DrdI         1684  GACCAGAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1091  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2116  GAATTC               G^AATTC
EcoT22I      1969  ATGCAT               ATGCA^T
KasI         1372  GGCGCC               G^GCGCC
KspI           56  CCGCGG               CCGC^GG
MluI           17  ACGCGT               A^CGCGT
NaeI         1017  GCCGGC               GCC^GGC
NarI         1372  GGCGCC               GG^CGCC
NgoMIV       1017  GCCGGC               G^CCGGC
NsiI         1969  ATGCAT               ATGCA^T
PflFI         306  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         369  CCACCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII          56  CCGCGG               CCGC^GG
SfoI         1372  GGCGCC               GGC^GCC
SphI         1971  GCATGC               GCATG^C
SspBI         219  TGTACA               T^GTACA
Tth111I       306  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        369  CCACCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1376  CCATGTGCAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI         1162  GACGCC               G[GA]^CG[CT]C
             1372  GGCGCC              
AflIII         17  ACGCGT               A^C[GA][CT]GT
             1838  ACGTGT              
AlwI          288  GATCC                GGATC(4/5)
              486  GATCC               
AviII        1781  TGCGCA               TGC^GCA
             1946  TGCGCA              
BfaI           79  CTAG                 C^TAG
             1219  CTAG                
BsaHI        1162  GACGCC               G[GA]^CG[CT]C
             1372  GGCGCC              
BsgI         1666  CTGCAC               GTGCAG(16/14)
             1380  GTGCAG              
BsrBI          24  CCGCTC               CCGCTC(-3/-3)
              427  GAGCGG              
BsrFI        1017  GCCGGC               [GA]^CCGG[CT]
             2012  ACCGGC              
BstYI         485  AGATCC               [GA]^GATC[CT]
             1730  AGATCT              
Cfr10I       1017  GCCGGC               [GA]^CCGG[CT]
             2012  ACCGGC              
EcoNI         174  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2049  CCTGAATGAGG         
FspI         1781  TGCGCA               TGC^GCA
             1946  TGCGCA              
HaeII         539  AGCGCC               [GA]GCGC^[CT]
             1372  GGCGCC              
HgaI         1162  GACGC                GACGC(5/10)
             2042  GACGC               
Hin1I        1162  GACGCC               G[GA]^CG[CT]C
             1372  GGCGCC              
HinfI         272  GAGTC                G^A[ACGT]TC
             1294  GAGTC               
MaeI           79  CTAG                 C^TAG
             1219  CTAG                
MaeII         140  ACGT                 A^CGT
             1838  ACGT                
MflI          485  AGATCC               [GA]^GATC[CT]
             1730  AGATCT              
NcoI         1505  CCATGG               C^CATGG
             1517  CCATGG              
NspI         1448  ACATGC               [GA]CATG^[CT]
             1971  GCATGC              
PleI          272  GAGTC                GAGTC(4/5)
             1294  GAGTC               
PpuMI        1043  AGGTCCT              [GA]G^G[AT]CC[CT]
             1070  AGGACCT             
SfiI          555  GGCCCTGATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1305  GGCCTTGGTGGCC       
SmlI         1360  CTCAAG               C^T[CT][GA]AG
             1822  CTCAAG              
StuI          474  AGGCCT               AGG^CCT
             1007  AGGCCT              
Tsp509I       965  AATT                 ^AATT
             2117  AATT                
XhoII         485  AGATCC               [GA]^GATC[CT]
             1730  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AvaII       BalI        BanI        BbvCI       BbvI        
BcgI        BcnI        BglI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       RsaI        
Sau3AI      Sau96I      SbfI        ScrFI       SfaNI       
SfcI        Sse8387I    StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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