Restriction map for pF1KB4102
 (Linear) (Six-base) MAPSORT of: KIBB4102.seq  Check: 792  from: 1  to: 2145



KIBB4102.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1679    2145 
   Size:       1679     466
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311    1277    2145 
   Size:        311     966     868

  Fragments arranged by size: 

                 966     868     311
 
AflIII A'CryG_T

Cuts at:      0      17    1838    2145 
   Size:         17    1821     307

  Fragments arranged by size: 

                1821     307      17
 
AleI CACnn'nnGTG

Cuts at:      0     739    1411    2073    2145 
   Size:        739     672     662      72

  Fragments arranged by size: 

                 739     672     662      72
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1102    2145 
   Size:       1102    1043
 
AlwNI CAG_nnn'CTG

Cuts at:      0     174     285     781     829     889    1122    1255    1681 
   Size:        174     111     496      48      60     233     133     426

Cuts at:   1681    2145 
   Size:        464

  Fragments arranged by size: 

                 496     464     426     233     174     133     111      60
                  48
 
ApoI r'AATT_y

Cuts at:      0    2116    2145 
   Size:       2116      29
 
BanI G'GyrC_C

Cuts at:      0     727    1014    1372    2145 
   Size:        727     287     358     773

  Fragments arranged by size: 

                 773     727     358     287
 
BanII G_rGCy'C

Cuts at:      0      38    2145 
   Size:         38    2107
 
BbeI G_GCGC'C

Cuts at:      0    1376    2145 
   Size:       1376     769
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1503    2145 
   Size:       1503     642
 
BbvCI CC'TCA_GC

Cuts at:      0     822     890    1075    2145 
   Size:        822      68     185    1070

  Fragments arranged by size: 

                1070     822     185      68
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1431    1465    1548    1582    2090    2124    2145 
   Size:       1431      34      83      34     508      34      21

  Fragments arranged by size: 

                1431     508      83      34      34      34      21
 
BfrBI ATG'CAT

Cuts at:      0    1971    2145 
   Size:       1971     174
 
BglI GCCn_nnn'nGGC

Cuts at:      0     115     170     214     562    1095    1312    2145 
   Size:        115      55      44     348     533     217     833

  Fragments arranged by size: 

                 833     533     348     217     115      55      44
 
BglII A'GATC_T

Cuts at:      0    1730    2145 
   Size:       1730     415
 
Bme1580I G_kGCm'C

Cuts at:      0     719     905    1185    1535    1561    2145 
   Size:        719     186     280     350      26     584

  Fragments arranged by size: 

                 719     584     350     280     186      26
 
BmgBI CAC'GTC

Cuts at:      0    1839    2145 
   Size:       1839     306
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     440    1566    2067    2145 
   Size:        440    1126     501      78

  Fragments arranged by size: 

                1126     501     440      78
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1977    2082    2145 
   Size:       1977     105      63

  Fragments arranged by size: 

                1977     105      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     196     262     318     651     672    1144    2145 
   Size:        175      21      66      56     333      21     472    1001

  Fragments arranged by size: 

                1001     472     333     175      66      56      21      21
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     822     890     955    1075    1708    2145 
   Size:        822      68      65     120     633     437

  Fragments arranged by size: 

                 822     633     437     120      68      65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    1807    2145 
   Size:       1345     462     338

  Fragments arranged by size: 

                1345     462     338
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      58     298     539    2056    2145 
   Size:         58     240     241    1517      89

  Fragments arranged by size: 

                1517     241     240      89      58
 
BsaAI yAC'GTr

Cuts at:      0     141    2145 
   Size:        141    2004
 
BsaHI Gr'CG_yC

Cuts at:      0    1163    1373    2145 
   Size:       1163     210     772

  Fragments arranged by size: 

                1163     772     210
 
BsaWI w'CCGG_w

Cuts at:      0     296    2145 
   Size:        296    1849
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1514    1544    2145 
   Size:       1514      30     601

  Fragments arranged by size: 

                1514     601      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     615     889    1039    1237    1348    2071    2074    2145 
   Size:        615     274     150     198     111     723       3      71

  Fragments arranged by size: 

                 723     615     274     198     150     111      71       3
            
 
BseYI C'CCAG_C

Cuts at:      0     418    1166    2127    2140    2145 
   Size:        418     748     961      13       5

  Fragments arranged by size: 

                 961     748     418      13       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1401    1651    2145 
   Size:       1401     250     494

  Fragments arranged by size: 

                1401     494     250
 
BsiHKAI G_wGCw'C

Cuts at:      0    1553    2145 
   Size:       1553     592
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     124    2145 
   Size:        124    2021
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     719     905    1185    1535    1553    1561    2145 
   Size:         38     681     186     280     350      18       8     584

  Fragments arranged by size: 

                 681     584     350     280     186      38      18       8
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     164    1032    1089    1655    1673    1679    2006    2033 
   Size:        164     868      57     566      18       6     327      27

Cuts at:   2033    2145 
   Size:        112

  Fragments arranged by size: 

                 868     566     327     164     112      57      27      18
                   6
 
BsrBI CCG'CTC

Cuts at:      0      26     429    2145 
   Size:         26     403    1716

  Fragments arranged by size: 

                1716     403      26
 
BsrFI r'CCGG_y

Cuts at:      0    1017    2012    2145 
   Size:       1017     995     133

  Fragments arranged by size: 

                1017     995     133
 
BsrGI T'GTAC_A

Cuts at:      0     219    2145 
   Size:        219    1926
 
BssHII G'CGCG_C

Cuts at:      0       8      92     118    2145 
   Size:          8      84      26    2027

  Fragments arranged by size: 

                2027      84      26       8
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      45    2145 
   Size:         45    2100
 
BstYI r'GATC_y

Cuts at:      0     485    1730    2145 
   Size:        485    1245     415

  Fragments arranged by size: 

                1245     485     415
 
Bsu36I CC'TnA_GG

Cuts at:      0    1092    2145 
   Size:       1092    1053
 
BtgI C'CryG_G

Cuts at:      0      56    1505    1517    2145 
   Size:         56    1449      12     628

  Fragments arranged by size: 

                1449     628      56      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1902    2145 
   Size:       1902     243
 
BtsI GCAGTG_nn'

Cuts at:      0     129    1342    1535    2145 
   Size:        129    1213     193     610

  Fragments arranged by size: 

                1213     610     193     129
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1690    2145 
   Size:       1690     455
 
EaeI y'GGCC_r

Cuts at:      0     107     579     977    1019    1502    1853    2145 
   Size:        107     472     398      42     483     351     292

  Fragments arranged by size: 

                 483     472     398     351     292     107      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      46    2145 
   Size:         46    2099
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     196     262     311     318     651     672    1144 
   Size:        175      21      66      49       7     333      21     472

Cuts at:   1144    1277    2145 
   Size:        133     868

  Fragments arranged by size: 

                 868     472     333     175     133      66      49      21
                  21       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     178    2053    2145 
   Size:        178    1875      92

  Fragments arranged by size: 

                1875     178      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0     171     322     555    1044    1071    2145 
   Size:        171     151     233     489      27    1074

  Fragments arranged by size: 

                1074     489     233     171     151      27
 
EcoRI G'AATT_C

Cuts at:      0    2116    2145 
   Size:       2116      29
 
FspI TGC'GCA

Cuts at:      0    1783    1948    2145 
   Size:       1783     165     197

  Fragments arranged by size: 

                1783     197     165
 
FspAI rTGC'GCAy

Cuts at:      0    1783    2145 
   Size:       1783     362
 
HaeII r_GCGC'y

Cuts at:      0     543    1376    2145 
   Size:        543     833     769

  Fragments arranged by size: 

                 833     769     543
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     295     327    1279    1311    2145 
   Size:        295      32     952      32     834

  Fragments arranged by size: 

                 952     834     295      32      32
 
KasI G'GCGC_C

Cuts at:      0    1372    2145 
   Size:       1372     773
 
MluI A'CGCG_T

Cuts at:      0      17    2145 
   Size:         17    2128
 
MscI TGG'CCA

Cuts at:      0     581     979    1504    2145 
   Size:        581     398     525     641

  Fragments arranged by size: 

                 641     581     525     398
 
MslI CAynn'nnrTG

Cuts at:      0     232     377     739     991    1411    1721    1882    1906 
   Size:        232     145     362     252     420     310     161      24

Cuts at:   1906    2073    2145 
   Size:        167      72

  Fragments arranged by size: 

                 420     362     310     252     232     167     161     145
                  72      24
 
MspA1I CmG'CkG

Cuts at:      0      58     620     692     889    1211    1626    1678    1861 
   Size:         58     562      72     197     322     415      52     183

Cuts at:   1861    2145 
   Size:        284

  Fragments arranged by size: 

                 562     415     322     284     197     183      72      58
                  52
 
NaeI GCC'GGC

Cuts at:      0    1019    2145 
   Size:       1019    1126
 
NarI GG'CG_CC

Cuts at:      0    1373    2145 
   Size:       1373     772
 
NcoI C'CATG_G

Cuts at:      0    1505    1517    2145 
   Size:       1505      12     628

  Fragments arranged by size: 

                1505     628      12
 
NgoMIV G'CCGG_C

Cuts at:      0    1017    2145 
   Size:       1017    1128
 
NsiI A_TGCA'T

Cuts at:      0    1973    2145 
   Size:       1973     172
 
NspI r_CATG'y

Cuts at:      0    1452    1975    2145 
   Size:       1452     523     170

  Fragments arranged by size: 

                1452     523     170
 
PasI CC'CwG_GG

Cuts at:      0     846    2145 
   Size:        846    1299
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     375    2145 
   Size:        375    1770
 
PpuMI rG'GwC_Cy

Cuts at:      0    1044    1071    2145 
   Size:       1044      27    1074

  Fragments arranged by size: 

                1074    1044      27
 
PstI C_TGCA'G

Cuts at:      0     170     390     795    1835    2003    2030    2145 
   Size:        170     220     405    1040     168      27     115

  Fragments arranged by size: 

                1040     405     220     170     168     115      27
 
PvuII CAG'CTG

Cuts at:      0     692     889    1626    1678    1861    2145 
   Size:        692     197     737      52     183     284

  Fragments arranged by size: 

                 737     692     284     197     183      52
 
SacII CC_GC'GG

Cuts at:      0      59    2145 
   Size:         59    2086
 
SbfI CC_TGCA'GG

Cuts at:      0     170    1835    2030    2145 
   Size:        170    1665     195     115

  Fragments arranged by size: 

                1665     195     170     115
 
SfcI C'TryA_G

Cuts at:      0     166     386     791    1430    1831    1976    1999    2026 
   Size:        166     220     405     639     401     145      23      27

Cuts at:   2026    2145 
   Size:        119

  Fragments arranged by size: 

                 639     405     401     220     166     145     119      27
                  23
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     562    1312    2145 
   Size:        562     750     833

  Fragments arranged by size: 

                 833     750     562
 
SfoI GGC'GCC

Cuts at:      0    1374    2145 
   Size:       1374     771
 
SmlI C'TyrA_G

Cuts at:      0    1360    1822    2145 
   Size:       1360     462     323

  Fragments arranged by size: 

                1360     462     323
 
SphI G_CATG'C

Cuts at:      0    1975    2145 
   Size:       1975     170
 
StuI AGG'CCT

Cuts at:      0     476    1009    2145 
   Size:        476     533    1136

  Fragments arranged by size: 

                1136     533     476
 
StyI C'CwwG_G

Cuts at:      0     767    1307    1505    1517    1607    2145 
   Size:        767     540     198      12      90     538

  Fragments arranged by size: 

                 767     540     538     198      90      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     528     714     745    2145 
   Size:        528     186      31    1400

  Fragments arranged by size: 

                1400     528     186      31
 
TatI w'GTAC_w

Cuts at:      0     219     251    2145 
   Size:        219      32    1894

  Fragments arranged by size: 

                1894     219      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1514    1546    1802    1834    2145 
   Size:       1514      32     256      32     311

  Fragments arranged by size: 

                1514     311     256      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     309    2145 
   Size:        309    1836
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1383    2145 
   Size:       1383     762
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I
     KasI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PasI    PflMI    PpuMI     PstI    PvuII
    SacII     SbfI     SfcI     SfiI     SfoI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BciVI     BclI     BlpI     BmtI    BsaBI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI     EarI
  EcoICRI    EcoRV     FalI     FseI   HincII  HindIII     HpaI     KpnI
     MfeI     MmeI     NdeI     NheI     NotI     NruI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI     ScaI
    SexAI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI     XbaI     XhoI     XmaI     XmnI     ZraI
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