Restriction map for pF1KB4066
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       AccI        AccII       AcyI        AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AspEI       AspHI       
AspI        AvaI        AvrII       BalI        BbeI        
BbsI        BbvCI       BcgI        BciVI       BclI        
BlnI        BpuAI       BsaBI       BsaHI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PpuMI       PshAI       PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StyI        SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1120  GGTACC               G^GTACC
AccIII        300  TCCGGA               T^CCGGA
AclI           43  AACGTT               AA^CGTT
AflII         948  CTTAAG               C^TTAAG
AlwNI         157  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI         1162  ATTAAT               AT^TAAT
AsnI         1162  ATTAAT               AT^TAAT
Asp718I      1120  GGTACC               G^GTACC
AviII         834  TGCGCA               TGC^GCA
BamHI         657  GGATCC               G^GATCC
BanI         1120  GGTACC               G^G[CT][GA]CC
BbrPI          60  CACGTG               CAC^GTG
BfrI          948  CTTAAG               C^TTAAG
BglI          635  GCCTGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         900  AGATCT               A^GATCT
BlpI          753  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      753  GCTAAGC              GC^T[ACGT]AGC
BsaAI          60  CACGTG               [CT]AC^GT[GA]
BsaWI         300  TCCGGA               [AT]^CCGG[AT]
BseAI         300  TCCGGA               T^CCGGA
BseRI         130  GAGGAG               GAGGAG(10/8)
BsgI          439  CTGCAC               GTGCAG(16/14)
BsmFI         290  GGGAC                GGGAC(10/14)
BspEI         300  TCCGGA               T^CCGGA
BsrDI        1185  CATTGC               GCAATG(2/0)
CelII         753  GCTAAGC              GC^T[ACGT]AGC
CfoI          835  GCGC                 GCG^C
EarI          210  GAAGAG               CTCTTC(1/4)
FspI          834  TGCGCA               TGC^GCA
HgaI          391  GCGTC                GACGC(5/10)
HhaI          835  GCGC                 GCG^C
HinP1I        835  GCGC                 G^CGC
KpnI         1120  GGTACC               GGTAC^C
Ksp632I       210  GAAGAG               CTCTTC(1/4)
MroI          300  TCCGGA               T^CCGGA
MslI          442  CACCCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI          60  CACGTG               CAC^GTG
PmlI           60  CACGTG               CAC^GTG
PshBI        1162  ATTAAT               AT^TAAT
Psp1406I       43  AACGTT               AA^CGTT
PstI          742  CTGCAG               CTGCA^G
SapI          210  GAAGAGC              GCTCTTC(1/4)
XbaI          942  TCTAGA               T^CTAGA
XcmI          277  CCAGGTGTTTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AlwI          658  GATCC                GGATC(4/5)
              657  GGATC               
Asp700I       320  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1141  GAAGCCCTTC          
AvaII         510  GGACC                G^G[AT]CC
              558  GGACC               
BanII         133  GAGCCC               G[GA]GC[CT]^C
              189  GAGCCC              
BcnI          183  CCCGG                CC^[GC]GG
              444  CCCGG               
BfaI          388  CTAG                 C^TAG
              943  CTAG                
BmyI          133  GAGCCC               G[AGT]GC[ACT]^C
              189  GAGCCC              
Bsp1286I      133  GAGCCC               G[AGT]GC[ACT]^C
              189  GAGCCC              
Bsp1407I      546  TGTACA               T^GTACA
              819  TGTACA              
BsrGI         546  TGTACA               T^GTACA
              819  TGTACA              
BstAPI        744  GCAGACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1232  GCATCAAATGC         
BstYI         657  GGATCC               [GA]^GATC[CT]
              900  AGATCT              
Cac8I         248  GCAGGC               GC[ACGT]^[ACGT]GC
              644  GCCTGC              
DsaI          196  CCACGG               C^C[GA][CT]GG
              594  CCACGG              
MaeI          388  CTAG                 C^TAG
              943  CTAG                
MaeII          44  ACGT                 A^CGT
               61  ACGT                
MaeIII        224  GTGAC                ^GT[ACGT]AC
              451  GTGAC               
MboII         210  GAAGA                GAAGA(8/7)
              920  GAAGA               
MflI          657  GGATCC               [GA]^GATC[CT]
              900  AGATCT              
NciI          183  CCCGG                CC^[GC]GG
              444  CCCGG               
NspI          816  GCATGT               [GA]CATG^[CT]
              837  GCATGT              
PflMI         513  CCAACCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1195  CCAACTATTGG         
PleI          292  GACTC                GAGTC(4/5)
              400  GAGTC               
PvuII         759  CAGCTG               CAG^CTG
              859  CAGCTG              
SfaNI         923  GATGC                GCATC(5/9)
             1232  GCATC               
SmlI          470  CTCAAG               C^T[CT][GA]AG
              948  CTTAAG              
SspBI         546  TGTACA               T^GTACA
              819  TGTACA              
StuI          250  AGGCCT               AGG^CCT
              642  AGGCCT              
TaqI          264  TCGA                 T^CGA
              565  TCGA                
Tsp45I        224  GTGAC                ^GT[GC]AC
              451  GTGAC               
TthHB8I       264  TCGA                 T^CGA
              565  TCGA                
Van91I        513  CCAACCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1195  CCAACTATTGG         
XhoII         657  GGATCC               [GA]^GATC[CT]
              900  AGATCT              
XmnI          320  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1141  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BpmI        BsaI        BsaJI       BsiYI       
BslI        BsmAI       BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MboI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       
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