Restriction map for pF1KB4019
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbsI        BbvCI       
BcgI        BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII         698  CGCG                 CG^CG
AflIII       1016  ACATGT               A^C[GA][CT]GT
AlwI          672  GATCC                GGATC(4/5)
ApaI         1079  GGGCCC               GGGCC^C
AspHI         651  GAGCAC               G[AT]GC[AT]^C
BcnI           58  CCCGG                CC^[GC]GG
BsaWI         866  TCCGGT               [AT]^CCGG[AT]
BsgI         1048  GTGCAG               GTGCAG(16/14)
BsiHKAI       651  GAGCAC               G[AT]GC[AT]^C
BsmBI         708  GAGACG               CGTCTC(1/5)
Bsp1407I     1178  TGTACA               T^GTACA
BspHI        1040  TCATGA               T^CATGA
BspLU11I     1016  ACATGT               A^CATGT
BspMI         415  GCAGGT               ACCTGC(4/8)
BsrBI         592  GAGCGG               CCGCTC(-3/-3)
BsrGI        1178  TGTACA               T^GTACA
BstUI         698  CGCG                 CG^CG
DraI          201  TTTAAA               TTT^AAA
DsaI          954  CCATGG               C^C[GA][CT]GG
EaeI           47  TGGCCG               [CT]^GGCC[GA]
EarI          705  GAAGAG               CTCTTC(1/4)
EcoNI        1249  CCTACAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I        22  ATGCAT               ATGCA^T
Ksp632I       705  GAAGAG               CTCTTC(1/4)
MvnI          698  CGCG                 CG^CG
NciI           58  CCCGG                CC^[GC]GG
NcoI          954  CCATGG               C^CATGG
NsiI           22  ATGCAT               ATGCA^T
PflMI         165  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1160  GACTC                GAGTC(4/5)
RcaI         1040  TCATGA               T^CATGA
SfcI         1250  CTACAG               C^T[GA][CT]AG
SmlI          643  CTCAAG               C^T[CT][GA]AG
SspBI        1178  TGTACA               T^GTACA
StuI          509  AGGCCT               AGG^CCT
Van91I        165  CCAGAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          714  GAATTT               [GA]^AATT[CT]
              889  AAATTT              
AfaI          870  GTAC                 GT^AC
             1179  GTAC                
ApoI          714  GAATTT               [GA]^AATT[CT]
              889  AAATTT              
AvaII          95  GGACC                G^G[AT]CC
              563  GGACC               
BanII         281  GAGCCC               G[GA]GC[CT]^C
             1079  GGGCCC              
BbrPI         532  CACGTG               CAC^GTG
              654  CACGTG              
BsaAI         532  CACGTG               [CT]AC^GT[GA]
              654  CACGTG              
BsmFI          94  GGGAC                GGGAC(10/14)
               66  GTCCC               
BsmI           24  GCATTC               GAATGC(1/-1)
              438  GCATTC              
CfoI          181  GCGC                 GCG^C
              697  GCGC                
DpnII         468  GATC                 ^GATC
              672  GATC                
EcoT14I       954  CCATGG               C^C[AT][AT]GG
             1074  CCAAGG              
FauI          110  GCGGG                CCCGC(4/6)
              352  GCGGG               
HhaI          181  GCGC                 GCG^C
              697  GCGC                
HinP1I        181  GCGC                 G^CGC
              697  GCGC                
HphI          666  GGTGA                GGTGA(8/7)
              524  TCACC               
MboI          468  GATC                 ^GATC
              672  GATC                
NdeII         468  GATC                 ^GATC
              672  GATC                
PmaCI         532  CACGTG               CAC^GTG
              654  CACGTG              
PmlI          532  CACGTG               CAC^GTG
              654  CACGTG              
PvuII         310  CAGCTG               CAG^CTG
              926  CAGCTG              
RsaI          870  GTAC                 GT^AC
             1179  GTAC                
Sau3AI        468  GATC                 ^GATC
              672  GATC                
SspI          749  AATATT               AAT^ATT
              907  AATATT              
StyI          954  CCATGG               C^C[AT][AT]GG
             1074  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        BbvI        BciVI       BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MaeII       
MaeIII      MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NlaIII      
NlaIV       NspI        Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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