Restriction map for pF1KB4019
 (Linear) (Six-base) MAPSORT of: KIBB4019.seq  Check: 6916  from: 1  to: 1290



KIBB4019.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AflIII A'CryG_T

Cuts at:      0    1016    1290 
   Size:       1016     274
 
AleI CACnn'nnGTG

Cuts at:      0     532    1290 
   Size:        532     758
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      47    1290 
   Size:         47    1243
 
ApaI G_GGCC'C

Cuts at:      0    1083    1290 
   Size:       1083     207
 
ApoI r'AATT_y

Cuts at:      0     714     889    1290 
   Size:        714     175     401

  Fragments arranged by size: 

                 714     401     175
 
BanII G_rGCy'C

Cuts at:      0     285    1083    1290 
   Size:        285     798     207

  Fragments arranged by size: 

                 798     285     207
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     283    1107    1141    1290 
   Size:        283     824      34     149

  Fragments arranged by size: 

                 824     283     149      34
 
BfrBI ATG'CAT

Cuts at:      0      24    1290 
   Size:         24    1266
 
Bme1580I G_kGCm'C

Cuts at:      0    1083    1290 
   Size:       1083     207
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     776    1290 
   Size:        776     514
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     475    1290 
   Size:        475     815
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     514    1049    1290 
   Size:        428      86     535     241

  Fragments arranged by size: 

                 535     428     241      86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1290 
   Size:        628     662
 
BsaAI yAC'GTr

Cuts at:      0     534     656    1290 
   Size:        534     122     634

  Fragments arranged by size: 

                 634     534     122
 
BsaWI w'CCGG_w

Cuts at:      0     866    1290 
   Size:        866     424
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1082    1112    1290 
   Size:       1082      30     178

  Fragments arranged by size: 

                1082     178      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     293     304     814    1290 
   Size:        293      11     510     476

  Fragments arranged by size: 

                 510     476     293      11
 
BseYI C'CCAG_C

Cuts at:      0      34      68     308    1290 
   Size:         34      34     240     982

  Fragments arranged by size: 

                 982     240      34      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1069    1290 
   Size:       1069     221
 
BsiHKAI G_wGCw'C

Cuts at:      0     655    1290 
   Size:        655     635
 
BsmI GAATG_Cn'

Cuts at:      0      24     438    1290 
   Size:         24     414     852

  Fragments arranged by size: 

                 852     414      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     702    1290 
   Size:        702     588
 
Bsp1286I G_dGCh'C

Cuts at:      0     285     655    1083    1290 
   Size:        285     370     428     207

  Fragments arranged by size: 

                 428     370     285     207
 
BspHI T'CATG_A

Cuts at:      0    1040    1290 
   Size:       1040     250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     406    1290 
   Size:        406     884
 
BsrBI CCG'CTC

Cuts at:      0     594    1290 
   Size:        594     696
 
BsrGI T'GTAC_A

Cuts at:      0    1178    1290 
   Size:       1178     112
 
BtgI C'CryG_G

Cuts at:      0     954    1290 
   Size:        954     336
 
BtsI GCAGTG_nn'

Cuts at:      0     932    1272    1290 
   Size:        932     340      18

  Fragments arranged by size: 

                 932     340      18
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     798     833    1290 
   Size:        798      35     457

  Fragments arranged by size: 

                 798     457      35
 
DraI TTT'AAA

Cuts at:      0     203    1290 
   Size:        203    1087
 
EaeI y'GGCC_r

Cuts at:      0      47    1290 
   Size:         47    1243
 
EarI CTCTTCn'nnn_

Cuts at:      0     700    1290 
   Size:        700     590
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     383     717    1290 
   Size:        383     334     573

  Fragments arranged by size: 

                 573     383     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     514    1049    1290 
   Size:        428      86     535     241

  Fragments arranged by size: 

                 535     428     241      86
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1253    1290 
   Size:       1253      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0     355    1079    1258    1290 
   Size:        355     724     179      32

  Fragments arranged by size: 

                 724     355     179      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     243    1290 
   Size:        243    1047
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1167    1199    1290 
   Size:       1167      32      91

  Fragments arranged by size: 

                1167      91      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1068    1290 
   Size:       1068     222
 
MslI CAynn'nnrTG

Cuts at:      0      29     532     634    1045    1290 
   Size:         29     503     102     411     245

  Fragments arranged by size: 

                 503     411     245     102      29
 
MspA1I CmG'CkG

Cuts at:      0     312     385     394     928    1290 
   Size:        312      73       9     534     362

  Fragments arranged by size: 

                 534     362     312      73       9
 
NcoI C'CATG_G

Cuts at:      0     954    1290 
   Size:        954     336
 
NsiI A_TGCA'T

Cuts at:      0      26    1290 
   Size:         26    1264
 
NspI r_CATG'y

Cuts at:      0     199     333    1020    1290 
   Size:        199     134     687     270

  Fragments arranged by size: 

                 687     270     199     134
 
PciI A'CATG_T

Cuts at:      0    1016    1290 
   Size:       1016     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     171    1290 
   Size:        171    1119
 
PmlI CAC'GTG

Cuts at:      0     534     656    1290 
   Size:        534     122     634

  Fragments arranged by size: 

                 634     534     122
 
PspOMI G'GGCC_C

Cuts at:      0    1079    1290 
   Size:       1079     211
 
PvuII CAG'CTG

Cuts at:      0     312     928    1290 
   Size:        312     616     362

  Fragments arranged by size: 

                 616     362     312
 
SfcI C'TryA_G

Cuts at:      0    1250    1290 
   Size:       1250      40
 
SmlI C'TyrA_G

Cuts at:      0     643    1290 
   Size:        643     647
 
SspI AAT'ATT

Cuts at:      0     751     909    1290 
   Size:        751     158     381

  Fragments arranged by size: 

                 751     381     158
 
StuI AGG'CCT

Cuts at:      0     511    1290 
   Size:        511     779
 
StyI C'CwwG_G

Cuts at:      0     954    1074    1290 
   Size:        954     120     216

  Fragments arranged by size: 

                 954     216     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     112     544    1290 
   Size:        112     432     746

  Fragments arranged by size: 

                 746     432     112
 
TatI w'GTAC_w

Cuts at:      0    1178    1290 
   Size:       1178     112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1082    1114    1290 
   Size:       1082      32     176

  Fragments arranged by size: 

                1082     176      32
 

 Enzymes that do cut:

   AflIII     AleI     AlfI     ApaI     ApoI    BanII    BciVI    BfrBI
 Bme1580I     BmrI     BplI     BpmI    BpuEI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrGI     BtgI     BtsI    CspCI     DraI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I     MmeI
     MslI   MspA1I     NcoI     NsiI     NspI     PciI    PflMI     PmlI
   PspOMI    PvuII     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I
     BsaI    BsaBI    BsaHI    BsiEI    BsiWI    BspEI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI   DraIII     DrdI     EagI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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