Restriction map for pF1KB4009
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

Acc65I      AclI        AhdI        Alw44I      AlwI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AvrII       BalI        BamHI       BbvCI       BcgI        
BglII       BlnI        BsaBI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EcoRV       EcoT22I     
FseI        HindIII     HpaI        KpnI        MamI        
MfeI        MflI        MluI        MluNI       MscI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PmeI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
ScaI        SfiI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII        1327  GACGTC               GACGT^C
AccI         2062  GTCTAC               GT^[AC][GT]AC
AccIII       1155  TCCGGA               T^CCGGA
AflII         255  CTTAAG               C^TTAAG
AflIII       1202  ACACGT               A^C[GA][CT]GT
AgeI         2304  ACCGGT               A^CCGGT
AlwNI        1480  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1420  AGCGCT               AGC^GCT
ApaI         2187  GGGCCC               GGGCC^C
Asp700I       856  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1871  TGCGCA               TGC^GCA
BbeI         1594  GGCGCC               GGCGC^C
BbrPI        1006  CACGTG               CAC^GTG
BclI          381  TGATCA               T^GATCA
BfrI          255  CTTAAG               C^TTAAG
BglI         1567  GCCCTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1887  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1887  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1006  CACGTG               [CT]AC^GT[GA]
BseAI        1155  TCCGGA               T^CCGGA
BsiEI        1736  CGGCCG               CG[GA][CT]^CG
BspEI        1155  TCCGGA               T^CCGGA
BstAPI         36  GCAAGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2366  TTCGAA               TT^CGAA
BstEII       1755  GGTGACC              G^GT[ACGT]ACC
BstPI        1755  GGTGACC              G^GT[ACGT]ACC
BstXI        1760  CCAAGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1887  GCTGAGC              GC^T[ACGT]AGC
DpnII         382  GATC                 ^GATC
EagI         1736  CGGCCG               C^GGCCG
EclXI        1736  CGGCCG               C^GGCCG
Eco47III     1420  AGCGCT               AGC^GCT
Eco52I       1736  CGGCCG               C^GGCCG
EcoNI         677  CCTTGGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1755  GGTGACC              G^GT[ACGT]ACC
EcoRI        2287  GAATTC               G^AATTC
FbaI          381  TGATCA               T^GATCA
FspI         1871  TGCGCA               TGC^GCA
HincII       1456  GTCAAC               GT[CT]^[GA]AC
HindII       1456  GTCAAC               GT[CT]^[GA]AC
KasI         1594  GGCGCC               G^GCGCC
KspI         1605  CCGCGG               CCGC^GG
MboI          382  GATC                 ^GATC
MroI         1155  TCCGGA               T^CCGGA
NarI         1594  GGCGCC               GG^CGCC
NdeII         382  GATC                 ^GATC
NlaIII        847  CATG                 CATG^
NspV         2366  TTCGAA               TT^CGAA
PflMI        1200  CCACACGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2304  ACCGGT               A^CCGGT
PmaCI        1006  CACGTG               CAC^GTG
PmlI         1006  CACGTG               CAC^GTG
SacII        1605  CCGCGG               CCGC^GG
Sau3AI        382  GATC                 ^GATC
SbfI         1569  CCTGCAGG             CCTGCA^GG
SexAI        1751  ACCTGGT              A^CC[AT]GGT
SfoI         1594  GGCGCC               GGC^GCC
SfuI         2366  TTCGAA               TT^CGAA
Sse8387I     1569  CCTGCAGG             CCTGCA^GG
Van91I       1200  CCACACGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1907  CCAAGCACAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          856  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          721  GAATTT               [GA]^AATT[CT]
             2287  GAATTC              
ApoI          721  GAATTT               [GA]^AATT[CT]
             2287  GAATTC              
AseI          703  ATTAAT               AT^TAAT
              880  ATTAAT              
AsnI          703  ATTAAT               AT^TAAT
              880  ATTAAT              
BbsI         1159  GAAGAC               GAAGAC(2/6)
             2091  GTCTTC              
BciVI        1280  GGATAC               GTATCC(6/5)
             1106  GTATCC              
BpmI           90  CTGGAG               CTGGAG(16/14)
              893  CTGGAG              
BpuAI        1159  GAAGAC               GAAGAC(2/6)
             2091  GTCTTC              
BsaWI        1155  TCCGGA               [AT]^CCGG[AT]
             2304  ACCGGT              
BseRI         277  GAGGAG               GAGGAG(10/8)
             2145  GAGGAG              
BsrFI        2031  ACCGGC               [GA]^CCGG[CT]
             2304  ACCGGT              
Cfr10I       2031  ACCGGC               [GA]^CCGG[CT]
             2304  ACCGGT              
HgaI         1657  GACGC                GACGC(5/10)
             1900  GACGC               
MaeIII       1267  GTCAC                ^GT[ACGT]AC
             1756  GTGAC               
MslI          537  CATTAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1308  CACAACTGTG          
PshBI         703  ATTAAT               AT^TAAT
              880  ATTAAT              
PstI         1570  CTGCAG               CTGCA^G
             1693  CTGCAG              
PvuII         226  CAGCTG               CAG^CTG
             1813  CAGCTG              
SapI          607  GAAGAGC              GCTCTTC(1/4)
             2097  GCTCTTC             
SfaNI         764  GCATC                GCATC(5/9)
             2202  GCATC               
SmaI         1730  CCCGGG               CCC^GGG
             1834  CCCGGG              
SmlI          255  CTTAAG               C^T[CT][GA]AG
             1819  CTCAAG              
TfiI          488  GAATC                G^A[AT]TC
              750  GATTC               
Tsp45I       1267  GTCAC                ^GT[GC]AC
             1756  GTGAC               
XmaCI        1730  CCCGGG               C^CCGGG
             1834  CCCGGG              
XmaI         1730  CCCGGG               C^CCGGG
             1834  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AocI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BsaHI       BsaI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIV       PleI        PpuMI       RsaI        
SacI        Sau96I      ScrFI       SfcI        StyI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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