Restriction map for pF1KB3962
 (Circular) (Six-base) MAPSORT of: pF1KB3962.seq  Check: 4243  from: 1  to: 4628



pF1KB3962  4628 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    535    1666     535 
   Size:       1131    3497
 
Acc65I G'GTAC_C

Cuts at:   1644    1644 
   Size:       4628
 
AclI AA'CG_TT

Cuts at:   4468    4468 
   Size:       4628
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    389    1280    1514    2453    2885    3238    3900     389 
   Size:        891     234     939     432     353     662    1117

  Fragments arranged by size: 

                1117     939     891     662     432     353     234
 
AflIII A'CryG_T

Cuts at:    123    1980    3780    4061     123 
   Size:       1857    1800     281     690

  Fragments arranged by size: 

                1857    1800     690     281
 
AleI CACnn'nnGTG

Cuts at:    868     868 
   Size:       4628
 
AlwNI CAG_nnn'CTG

Cuts at:   1519    1567    1933    3371    1519 
   Size:         48     366    1438    2776

  Fragments arranged by size: 

                2776    1438     366      48
 
ApaLI G'TGCA_C

Cuts at:   3466    3466 
   Size:       4628
 
ApoI r'AATT_y

Cuts at:    112     129     268     391    1632    3981    4237     112 
   Size:         17     139     123    1241    2349     256     503

  Fragments arranged by size: 

                2349    1241     503     256     139     123      17
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4628
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4628
 
AvaI C'yCGr_G

Cuts at:   1648    1648 
   Size:       4628
 
BamHI G'GATC_C

Cuts at:   1653    1653 
   Size:       4628
 
BanI G'GyrC_C

Cuts at:    708     870    1644    2058    2306    2341     708 
   Size:        162     774     414     248      35    2995

  Fragments arranged by size: 

                2995     774     414     248     162      35
 
BanII G_rGCy'C

Cuts at:   1642    2672    1642 
   Size:       1030    3598
 
BbeI G_GCGC'C

Cuts at:   2062    2310    2062 
   Size:        248    4380
 
BbsI GAAGACnn'nnnn_

Cuts at:   1268    1430    1268 
   Size:        162    4466
 
BciVI GTATCCnnnnn_n'

Cuts at:   2526    3582    4628    2526 
   Size:       1056    1046    2526

  Fragments arranged by size: 

                2526    1056    1046
 
BclI T'GATC_A

Cuts at:    306    2148     306 
   Size:       1842    2786
 
BglII A'GATC_T

Cuts at:   2143    4180    2143 
   Size:       2037    2591
 
BlpI GC'TnA_GC

Cuts at:   1740    1740 
   Size:       4628
 
Bme1580I G_kGCm'C

Cuts at:   2253    2346    3470    2253 
   Size:         93    1124    3411

  Fragments arranged by size: 

                3411    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1596    1840    1921    2256    4400    1596 
   Size:        244      81     335    2144    1824

  Fragments arranged by size: 

                2144    1824     335     244      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2995    2995 
   Size:       4628
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    953    1076     953 
   Size:        123    4505
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    633     672    1287    1804    2683    3397    3695     633 
   Size:         39     615     517     879     714     298    1566

  Fragments arranged by size: 

                1566     879     714     615     517     298      39
 
BsaI GGTCTCn'nnnn_

Cuts at:   4332    4332 
   Size:       4628
 
BsaAI yAC'GTr

Cuts at:    283    1981     283 
   Size:       1698    2930
 
BsaBI GATnn'nnATC

Cuts at:      6    2142    4179       6 
   Size:       2136    2037     455

  Fragments arranged by size: 

                2136    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2059    2307    3009    4505    2059 
   Size:        248     702    1496    2182

  Fragments arranged by size: 

                2182    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1817    2338    3016    3427    3574    3848    1817 
   Size:        521     678     411     147     274    2597

  Fragments arranged by size: 

                2597     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1065    1095    1065 
   Size:         30    4598
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    493     493 
   Size:       4628
 
BseYI C'CCAG_C

Cuts at:    956    2053    2811    3476    3884     956 
   Size:       1097     758     665     408    1700

  Fragments arranged by size: 

                1700    1097     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    560     560 
   Size:       4628
 
BsiEI CG_ry'CG

Cuts at:    104    1042    2170    2216    3446     104 
   Size:        938    1128      46    1230    1286

  Fragments arranged by size: 

                1286    1230    1128     938      46
 
BsiHKAI G_wGCw'C

Cuts at:   1642    2420    2610    3470    1642 
   Size:        778     190     860    2800

  Fragments arranged by size: 

                2800     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1642    2253    2346    2420    2610    2672    3470    1642 
   Size:        611      93      74     190      62     798    2800

  Fragments arranged by size: 

                2800     798     611     190      93      74      62
 
BspHI T'CATG_A

Cuts at:   3060    3060 
   Size:       4628
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1678    1892    2194    2575    1678 
   Size:        214     302     381    3731

  Fragments arranged by size: 

                3731     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2920    2974    4458    4623      50 
   Size:       2870      54    1484     165      55

  Fragments arranged by size: 

                2870    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2540    2540 
   Size:       4628
 
BsrFI r'CCGG_y

Cuts at:   2626    2807    2626 
   Size:        181    4447
 
BssSI C'ACGA_G

Cuts at:   2899    3607    2899 
   Size:        708    3920
 
BstAPI GCAn_nnn'nTGC

Cuts at:    574    2005     574 
   Size:       1431    3197
 
BstBI TT'CG_AA

Cuts at:   2989    2989 
   Size:       4628
 
BstYI r'GATC_y

Cuts at:      1    1155    1653    2120    2143    2478    2724    3030    3042 
   Size:       1154     498     467      23     335     246     306      12

Cuts at:   3042    3128    4180       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1154    1052     498     467     449     335     306     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1614    1614 
   Size:       4628
 
BtgI C'CryG_G

Cuts at:    866     866 
   Size:       4628
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2578    2759    2922    2578 
   Size:        181     163    4284

  Fragments arranged by size: 

                4284     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1871    1871 
   Size:       4628
 
DraI TTT'AAA

Cuts at:   1627    1627 
   Size:       4628
 
DrdI GACnn_nn'nnGTC

Cuts at:   2334    3678    2334 
   Size:       1344    3284
 
EaeI y'GGCC_r

Cuts at:    204    2213    2387    2778    2805    4150     204 
   Size:       2009     174     391      27    1345     682

  Fragments arranged by size: 

                2009    1345     682     391     174      27
 
EagI C'GGCC_G

Cuts at:   2213    2213 
   Size:       4628
 
EarI CTCTTCn'nnn_

Cuts at:    761    1134    2651    2861     761 
   Size:        373    1517     210    2528

  Fragments arranged by size: 

                2528    1517     373     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1564    2836    3578    3724    4438    1564 
   Size:       1272     742     146     714    1754

  Fragments arranged by size: 

                1754    1272     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1640    1640 
   Size:       4628
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    389     953    1076    1280    1514    2453    2885    3238    3900 
   Size:        564     123     204     234     939     432     353     662

Cuts at:   3900     389 
   Size:       1117

  Fragments arranged by size: 

                1117     939     662     564     432     353     234     204
                 123
 
EcoNI CCTnn'n_nnAGG

Cuts at:    720     740     720 
   Size:         20    4608
 
EcoO109I rG'GnC_Cy

Cuts at:    150     895    1767     150 
   Size:        745     872    3011

  Fragments arranged by size: 

                3011     872     745
 
EcoRI G'AATT_C

Cuts at:    129     391    1632     129 
   Size:        262    1241    3125

  Fragments arranged by size: 

                3125    1241     262
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2647    2647 
   Size:       4628
 
FspI TGC'GCA

Cuts at:   2409    2409 
   Size:       4628
 
HaeII r_GCGC'y

Cuts at:   2062    2310    3540    4311    2062 
   Size:        248    1230     771    2379

  Fragments arranged by size: 

                2379    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2470    2500    2502    2532    2470 
   Size:         30       2      30    4566

  Fragments arranged by size: 

                4566      30      30       2
 
HincII GTy'rAC

Cuts at:   1667    1667 
   Size:       4628
 
HindIII A'AGCT_T

Cuts at:    401     401 
   Size:       4628
 
KasI G'GCGC_C

Cuts at:   2058    2306    2058 
   Size:        248    4380
 
KpnI G_GTAC'C

Cuts at:   1648    1648 
   Size:       4628
 
MfeI C'AATT_G

Cuts at:    728     728 
   Size:       4628
 
MluI A'CGCG_T

Cuts at:   4061    4061 
   Size:       4628
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1114    3387    3571    1114 
   Size:       2273     184    2171

  Fragments arranged by size: 

                2273    2171     184
 
MscI TGG'CCA

Cuts at:    206    2389     206 
   Size:       2183    2445
 
MslI CAynn'nnrTG

Cuts at:    251     868    1432    1543    2617    2744     251 
   Size:        617     564     111    1074     127    2135

  Fragments arranged by size: 

                2135    1074     617     564     127     111
 
MspA1I CmG'CkG

Cuts at:    838    1287    1329    1739    2053    2413    3197    3442     838 
   Size:        449      42     410     314     360     784     245    2024

  Fragments arranged by size: 

                2024     784     449     410     360     314     245      42
            
 
NaeI GCC'GGC

Cuts at:   2809    2809 
   Size:       4628
 
NarI GG'CG_CC

Cuts at:   2059    2307    2059 
   Size:        248    4380
 
NcoI C'CATG_G

Cuts at:    866     866 
   Size:       4628
 
NgoMIV G'CCGG_C

Cuts at:   2807    2807 
   Size:       4628
 
NspI r_CATG'y

Cuts at:    127     832    1681    2181    3784     127 
   Size:        705     849     500    1603     971

  Fragments arranged by size: 

                1603     971     849     705     500
 
PciI A'CATG_T

Cuts at:    123    3780     123 
   Size:       3657     971
 
PfoI T'CCnGG_A

Cuts at:    882     882 
   Size:       4628
 
PmeI GTTT'AAAC

Cuts at:   1627    1627 
   Size:       4628
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3074    3106    3074 
   Size:         32    4596
 
PpuMI rG'GwC_Cy

Cuts at:    150     895     150 
   Size:        745    3883
 
PshAI GACnn'nnGTC

Cuts at:   1044    1044 
   Size:       4628
 
PstI C_TGCA'G

Cuts at:    843    1292    1675    2360     843 
   Size:        449     383     685    3111

  Fragments arranged by size: 

                3111     685     449     383
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4628
 
PvuII CAG'CTG

Cuts at:    838    1287    1329    2053    2413     838 
   Size:        449      42     724     360    3053

  Fragments arranged by size: 

                3053     724     449     360      42
 
RsrII CG'GwC_CG

Cuts at:   2823    2823 
   Size:       4628
 
SacI G_AGCT'C

Cuts at:   1642    1642 
   Size:       4628
 
SalI G'TCGA_C

Cuts at:   1665    1665 
   Size:       4628
 
SapI GCTCTTCn'nnn_

Cuts at:   1134    2651    2861    1134 
   Size:       1517     210    2901

  Fragments arranged by size: 

                2901    1517     210
 
SbfI CC_TGCA'GG

Cuts at:   1675    1675 
   Size:       4628
 
ScaI AGT'ACT

Cuts at:    693    4145     693 
   Size:       3452    1176
 
SfcI C'TryA_G

Cuts at:     33     839    1073    1288    1520    1671    2356    3324    3515 
   Size:        806     234     215     232     151     685     968     191

Cuts at:   3515      33 
   Size:       1146

  Fragments arranged by size: 

                1146     968     806     685     234     232     215     191
                 151
 
SfoI GGC'GCC

Cuts at:   2060    2308    2060 
   Size:        248    4380
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4628
 
SmaI CCC'GGG

Cuts at:   1650    1650 
   Size:       4628
 
SmlI C'TyrA_G

Cuts at:    648     687    1266    1783    2698    3412    3674     648 
   Size:         39     579     517     915     714     262    1602

  Fragments arranged by size: 

                1602     915     714     579     517     262      39
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4628
 
SphI G_CATG'C

Cuts at:   1681    2181    1681 
   Size:        500    4128
 
StyI C'CwwG_G

Cuts at:    866    1508    1762    2116     866 
   Size:        642     254     354    3378

  Fragments arranged by size: 

                3378     642     354     254
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    542     718    1464    1555    2211    2344    2803    3012     542 
   Size:        176     746      91     656     133     459     209    2158

  Fragments arranged by size: 

                2158     746     656     459     209     176     133      91
            
 
TatI w'GTAC_w

Cuts at:    187     691     915    1120    1558    4143     187 
   Size:        504     224     205     438    2585     672

  Fragments arranged by size: 

                2585     672     504     438     224     205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    150     182    4145    4177     150 
   Size:         32    3963      32     601

  Fragments arranged by size: 

                3963     601      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    292    1023    2425     292 
   Size:        731    1402    2495

  Fragments arranged by size: 

                2495    1402     731
 
XbaI T'CTAG_A

Cuts at:    432    1659     432 
   Size:       1227    3401
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1123    2123    1123 
   Size:       1000    3628
 
XmaI C'CCGG_G

Cuts at:   1648    1648 
   Size:       4628
 
XmnI GAAnn'nnTTC

Cuts at:    391     391 
   Size:       4628
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsiWI     BsmI    BsmBI    BspEI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI     HpaI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmlI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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