Restriction map for pF1KB3715
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tsp45I      
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccII         430  CGCG                 CG^CG
AcsI           37  AAATTC               [GA]^AATT[CT]
AflIII        504  ACGTGT               A^C[GA][CT]GT
AhdI          145  GACTTCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI           37  AAATTC               [GA]^AATT[CT]
AspEI         145  GACTTCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          325  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         592  GAGCCC               G[GA]GC[CT]^C
BcgI          492  GCAGCTTTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          592  GAGCCC               G[AGT]GC[ACT]^C
BsaI          460  GAGACC               GGTCTC(1/5)
BseRI         415  GAGGAG               GAGGAG(10/8)
BsiYI         311  CCTCTAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          311  CCTCTAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI          80  CGTCTC               CGTCTC(1/5)
Bsp1286I      592  GAGCCC               G[AGT]GC[ACT]^C
BssHII        429  GCGCGC               G^CGCGC
BstUI         430  CGCG                 CG^CG
BstXI         161  CCATGTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI          257  CCACGG               C^C[GA][CT]GG
EaeI          599  CGGCCA               [CT]^GGCC[GA]
Eam1105I      145  GACTTCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeIII        600  GGCC                 GG^CC
MslI          363  CATGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        206  CAGCTG               C[AC]G^C[GT]G
MvnI          430  CGCG                 CG^CG
PflFI         325  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          253  GACTC                GAGTC(4/5)
PstI          190  CTGCAG               CTGCA^G
PvuII         206  CAGCTG               CAG^CTG
SfcI          190  CTGCAG               C^T[GA][CT]AG
TfiI           24  GAATC                G^A[AT]TC
Tsp509I        38  AATT                 ^AATT
Tth111I       325  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII          78  GACGTC               GACGT^C
              328  GACGTC              
AlwI          234  GATCC                GGATC(4/5)
              320  GGATC               
AvaII         174  GGACC                G^G[AT]CC
              384  GGACC               
BbsI           75  GAAGAC               GAAGAC(2/6)
              471  GAAGAC              
BpuAI          75  GAAGAC               GAAGAC(2/6)
              471  GAAGAC              
BsaBI         405  GATAACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              564  GATCGACATC          
BsmAI         460  GAGAC                GTCTC(1/5)
               81  GTCTC               
BsmFI         200  GGGAC                GGGAC(10/14)
              251  GGGAC               
BsrI          475  ACTGG                ACTGG(1/-1)
              464  CCAGT               
BstF5I        246  CATCC                GGATG(2/0)
              560  GGATG               
Cac8I         429  GCGCGC               GC[ACGT]^[ACGT]GC
              601  GCCAGC              
CfoI          429  GCGC                 GCG^C
              431  GCGC                
Cfr13I        174  GGACC                G^G[ACGT]CC
              384  GGACC               
FokI          246  CATCC                GGATG(9/13)
              560  GGATG               
HhaI          429  GCGC                 GCG^C
              431  GCGC                
HinP1I        429  GCGC                 G^CGC
              431  GCGC                
HinfI          24  GAATC                G^A[ACGT]TC
              253  GACTC               
MamI          405  GATAACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              564  GATCGACATC          
MboII          75  GAAGA                GAAGA(8/7)
              471  GAAGA               
NlaIV         221  GGTTCC               GG[ACGT]^[ACGT]CC
              591  GGAGCC              
Sau96I        174  GGACC                G^G[ACGT]CC
              384  GGACC               
XcmI          126  CCAGGCAACCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              257  CCACGGTGGCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        BbvI        
BcnI        BsaHI       BsaJI       BssKI       BstNI       
DdeI        DpnII       EcoRII      Fnu4HI      HapII       
HgaI        Hin1I       HpaII       ItaI        MaeII       
MboI        MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      RsaI        Sau3AI      
ScrFI       TaqI        TseI        TspRI       TthHB8I     
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