Restriction map for pF1KB3666
 (Linear) (Six-base) MAPSORT of: KIBB3666.seq  Check: 6310  from: 1  to: 2214



KIBB3666.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1671    2214 
   Size:       1671     543
 
AatII G_ACGT'C

Cuts at:      0      41    2214 
   Size:         41    2173
 
AclI AA'CG_TT

Cuts at:      0     452    2214 
   Size:        452    1762
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1598    1820    2035    2214 
   Size:       1598     222     215     179

  Fragments arranged by size: 

                1598     222     215     179
 
AflII C'TTAA_G

Cuts at:      0     433    2214 
   Size:        433    1781
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1730    1762    2214 
   Size:       1730      32     452

  Fragments arranged by size: 

                1730     452      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1897    2113    2214 
   Size:       1897     216     101

  Fragments arranged by size: 

                1897     216     101
 
ApoI r'AATT_y

Cuts at:      0     313     356     389     632    1054    1133    2214 
   Size:        313      43      33     243     422      79    1081

  Fragments arranged by size: 

                1081     422     313     243      79      43      33
 
AvaI C'yCGr_G

Cuts at:      0    1919    2214 
   Size:       1919     295
 
BanI G'GyrC_C

Cuts at:      0      55     495    1659    2214 
   Size:         55     440    1164     555

  Fragments arranged by size: 

                1164     555     440      55
 
BanII G_rGCy'C

Cuts at:      0     118     545    1319    1679    2214 
   Size:        118     427     774     360     535

  Fragments arranged by size: 

                 774     535     427     360     118
 
BbeI G_GCGC'C

Cuts at:      0      59    2214 
   Size:         59    2155
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      33    1441    1628    2214 
   Size:         33    1408     187     586

  Fragments arranged by size: 

                1408     586     187      33
 
BbvCI CC'TCA_GC

Cuts at:      0    1697    2214 
   Size:       1697     517
 
BclI T'GATC_A

Cuts at:      0     507    2214 
   Size:        507    1707
 
BfrBI ATG'CAT

Cuts at:      0     921    2019    2214 
   Size:        921    1098     195

  Fragments arranged by size: 

                1098     921     195
 
BmtI G_CTAG'C

Cuts at:      0    1600    2214 
   Size:       1600     614
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1742    1791    2214 
   Size:         16    1726      49     423

  Fragments arranged by size: 

                1726     423      49      16
 
Bpu10I CC'TnA_GC

Cuts at:      0    1697    2214 
   Size:       1697     517
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255    2214 
   Size:        255    1959
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     174    2214 
   Size:        174    2040
 
BsaHI Gr'CG_yC

Cuts at:      0      38      56    2214 
   Size:         38      18    2158

  Fragments arranged by size: 

                2158      38      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1730    1760    2214 
   Size:       1730      30     454

  Fragments arranged by size: 

                1730     454      30
 
BseYI C'CCAG_C

Cuts at:      0    1318    2214 
   Size:       1318     896
 
BsiHKAI G_wGCw'C

Cuts at:      0     118     545    2214 
   Size:        118     427    1669

  Fragments arranged by size: 

                1669     427     118
 
BsmI GAATG_Cn'

Cuts at:      0    1784    2214 
   Size:       1784     430
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     545    1319    1679    2214 
   Size:        118     427     774     360     535

  Fragments arranged by size: 

                 774     535     427     360     118
 
BspHI T'CATG_A

Cuts at:      0    1344    1419    2214 
   Size:       1344      75     795

  Fragments arranged by size: 

                1344     795      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155    1671    1886    2214 
   Size:        155    1516     215     328

  Fragments arranged by size: 

                1516     328     215     155
 
BssSI C'ACGA_G

Cuts at:      0    1625    2214 
   Size:       1625     589
 
BstYI r'GATC_y

Cuts at:      0     648    2214 
   Size:        648    1566
 
Bsu36I CC'TnA_GG

Cuts at:      0     515    1265    2214 
   Size:        515     750     949

  Fragments arranged by size: 

                 949     750     515
 
BtgI C'CryG_G

Cuts at:      0     716    1013    2214 
   Size:        716     297    1201

  Fragments arranged by size: 

                1201     716     297
 
BtsI GCAGTG_nn'

Cuts at:      0     590    2214 
   Size:        590    1624
 
DraIII CAC_nnn'GTG

Cuts at:      0    1192    2214 
   Size:       1192    1022
 
EaeI y'GGCC_r

Cuts at:      0     866    2214 
   Size:        866    1348
 
EarI CTCTTCn'nnn_

Cuts at:      0     799     995    1307    1796    2054    2214 
   Size:        799     196     312     489     258     160

  Fragments arranged by size: 

                 799     489     312     258     196     160
 
EcoICRI GAG'CTC

Cuts at:      0     116     543    2214 
   Size:        116     427    1671

  Fragments arranged by size: 

                1671     427     116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1598    1742    1791    1820    2035    2214 
   Size:         16    1582     144      49      29     215     179

  Fragments arranged by size: 

                1582     215     179     144      49      29      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144    2214 
   Size:        144    2070
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1049    2065    2214 
   Size:       1049    1016     149

  Fragments arranged by size: 

                1049    1016     149
 
HaeII r_GCGC'y

Cuts at:      0      10      59    2214 
   Size:         10      49    2155

  Fragments arranged by size: 

                2155      49      10
 
HincII GTy'rAC

Cuts at:      0     774    2214 
   Size:        774    1440
 
HindIII A'AGCT_T

Cuts at:      0     334    2214 
   Size:        334    1880
 
HpaI GTT'AAC

Cuts at:      0     774    2214 
   Size:        774    1440
 
KasI G'GCGC_C

Cuts at:      0      55    2214 
   Size:         55    2159
 
MfeI C'AATT_G

Cuts at:      0    1502    2214 
   Size:       1502     712
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1065    1716    2214 
   Size:       1065     651     498

  Fragments arranged by size: 

                1065     651     498
 
MscI TGG'CCA

Cuts at:      0     868    2214 
   Size:        868    1346
 
MspA1I CmG'CkG

Cuts at:      0      72     107    1328    1618    1687    2073    2146    2214 
   Size:         72      35    1221     290      69     386      73      68

  Fragments arranged by size: 

                1221     386     290      73      72      69      68      35
            
 
NarI GG'CG_CC

Cuts at:      0      56    2214 
   Size:         56    2158
 
NcoI C'CATG_G

Cuts at:      0     716    2214 
   Size:        716    1498
 
NheI G'CTAG_C

Cuts at:      0    1596    2214 
   Size:       1596     618
 
NsiI A_TGCA'T

Cuts at:      0     923    2021    2214 
   Size:        923    1098     193

  Fragments arranged by size: 

                1098     923     193
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1192    2214 
   Size:       1192    1022
 
PfoI T'CCnGG_A

Cuts at:      0      32    2195    2214 
   Size:         32    2163      19

  Fragments arranged by size: 

                2163      32      19
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1730    1762    2214 
   Size:       1730      32     452

  Fragments arranged by size: 

                1730     452      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     144    2214 
   Size:        144    2070
 
PvuII CAG'CTG

Cuts at:      0      72    1618    2073    2146    2214 
   Size:         72    1546     455      73      68

  Fragments arranged by size: 

                1546     455      73      72      68
 
SacI G_AGCT'C

Cuts at:      0     118     545    2214 
   Size:        118     427    1669

  Fragments arranged by size: 

                1669     427     118
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1307    2214 
   Size:       1307     907
 
SfcI C'TryA_G

Cuts at:      0     500    1374    2214 
   Size:        500     874     840

  Fragments arranged by size: 

                 874     840     500
 
SfoI GGC'GCC

Cuts at:      0      57    2214 
   Size:         57    2157
 
SmlI C'TyrA_G

Cuts at:      0     270     433    2214 
   Size:        270     163    1781

  Fragments arranged by size: 

                1781     270     163
 
SspI AAT'ATT

Cuts at:      0    1069    1989    2214 
   Size:       1069     920     225

  Fragments arranged by size: 

                1069     920     225
 
StyI C'CwwG_G

Cuts at:      0     491     716    2214 
   Size:        491     225    1498

  Fragments arranged by size: 

                1498     491     225
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     462    2214 
   Size:        462    1752
 
TatI w'GTAC_w

Cuts at:      0    1387    2214 
   Size:       1387     827
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1515    1547    2214 
   Size:       1515      32     667

  Fragments arranged by size: 

                1515     667      32
 
XbaI T'CTAG_A

Cuts at:      0    1308    2214 
   Size:       1308     906
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1156    1434    2214 
   Size:       1156     278     780

  Fragments arranged by size: 

                1156     780     278
 
XmnI GAAnn'nnTTC

Cuts at:      0     425    1340    2214 
   Size:        425     915     874

  Fragments arranged by size: 

                 915     874     425
 
ZraI GAC'GTC

Cuts at:      0      39    2214 
   Size:         39    2175
 

 Enzymes that do cut:

     AarI    AatII     AclI     AcuI    AflII     AloI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BclI    BfrBI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BssSI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MmeI
     MscI   MspA1I     NarI     NcoI     NheI     NsiI    PflMI     PfoI
     PpiI    PpuMI    PvuII     SacI     SapI     SfcI     SfoI     SmlI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AfeI   AflIII     AgeI     AhdI     AleI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI    BsaAI    BsaBI    BsaWI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I     KpnI     MluI     MslI     NaeI     NdeI   NgoMIV
     NotI     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XhoI     XmaI
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