Restriction map for pF1KB3540
 (Linear) (Six-base) MAPSORT of: KIBB3540.seq  Check: 558  from: 1  to: 3525



KIBB3540.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AccI GT'mk_AC

Cuts at:      0     716    2917    3525 
   Size:        716    2201     608

  Fragments arranged by size: 

                2201     716     608
 
Acc65I G'GTAC_C

Cuts at:      0    2015    3525 
   Size:       2015    1510
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     390    1421    3525 
   Size:        390    1031    2104

  Fragments arranged by size: 

                2104    1031     390
 
AflIII A'CryG_T

Cuts at:      0    1772    3525 
   Size:       1772    1753
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1357    2121    3241    3525 
   Size:       1357     764    1120     284

  Fragments arranged by size: 

                1357    1120     764     284
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3012    3192    3525 
   Size:       3012     180     333

  Fragments arranged by size: 

                3012     333     180
 
ApoI r'AATT_y

Cuts at:      0      16     488     770    1537    1543    1828    1990    2209 
   Size:         16     472     282     767       6     285     162     219

Cuts at:   2209    2518    2817    3002    3433    3525 
   Size:        309     299     185     431      92

  Fragments arranged by size: 

                 767     472     431     309     299     285     282     219
                 185     162      92      16       6
 
BamHI G'GATC_C

Cuts at:      0     225    2354    3525 
   Size:        225    2129    1171

  Fragments arranged by size: 

                2129    1171     225
 
BanI G'GyrC_C

Cuts at:      0    2015    2155    3525 
   Size:       2015     140    1370

  Fragments arranged by size: 

                2015    1370     140
 
BanII G_rGCy'C

Cuts at:      0     602    2678    3525 
   Size:        602    2076     847

  Fragments arranged by size: 

                2076     847     602
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     562     903    1013    1106    1769    1819    2765    3525 
   Size:        562     341     110      93     663      50     946     760

  Fragments arranged by size: 

                 946     760     663     562     341     110      93      50
            
 
BbvCI CC'TCA_GC

Cuts at:      0    3098    3127    3525 
   Size:       3098      29     398

  Fragments arranged by size: 

                3098     398      29
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     990    1024    1840    1874    3450    3484    3525 
   Size:        990      34     816      34    1576      34      41

  Fragments arranged by size: 

                1576     990     816      41      34      34      34
 
BclI T'GATC_A

Cuts at:      0    1703    3080    3525 
   Size:       1703    1377     445

  Fragments arranged by size: 

                1703    1377     445
 
BglI GCCn_nnn'nGGC

Cuts at:      0     799    3525 
   Size:        799    2726
 
BglII A'GATC_T

Cuts at:      0     312     669    1275    3525 
   Size:        312     357     606    2250

  Fragments arranged by size: 

                2250     606     357     312
 
Bme1580I G_kGCm'C

Cuts at:      0    2597    3525 
   Size:       2597     928
 
BmgBI CAC'GTC

Cuts at:      0    2592    3525 
   Size:       2592     933
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     569     901    2591    3525 
   Size:        569     332    1690     934

  Fragments arranged by size: 

                1690     934     569     332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1469    3525 
   Size:       1469    2056
 
Bpu10I CC'TnA_GC

Cuts at:      0    3098    3127    3525 
   Size:       3098      29     398

  Fragments arranged by size: 

                3098     398      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     541    2328    2729    3525 
   Size:        424     117    1787     401     796

  Fragments arranged by size: 

                1787     796     424     401     117
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3110    3525 
   Size:       3110     415
 
BsaAI yAC'GTr

Cuts at:      0    2163    2203    3525 
   Size:       2163      40    1322

  Fragments arranged by size: 

                2163    1322      40
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1031    1061    1868    1898    2477    2507    3525 
   Size:       1031      30     807      30     579      30    1018

  Fragments arranged by size: 

                1031    1018     807     579      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94    1510    1534    3115    3525 
   Size:         94    1416      24    1581     410

  Fragments arranged by size: 

                1581    1416     410      94      24
 
BseYI C'CCAG_C

Cuts at:      0    1082    1464    1715    3185    3525 
   Size:       1082     382     251    1470     340

  Fragments arranged by size: 

                1470    1082     382     340     251
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2134    2856    3106    3525 
   Size:       2134     722     250     419

  Fragments arranged by size: 

                2134     722     419     250
 
BsiEI CG_ry'CG

Cuts at:      0     208    3525 
   Size:        208    3317
 
BsiHKAI G_wGCw'C

Cuts at:      0    2678    3234    3525 
   Size:       2678     556     291

  Fragments arranged by size: 

                2678     556     291
 
BsmI GAATG_Cn'

Cuts at:      0     187    3525 
   Size:        187    3338
 
Bsp1286I G_dGCh'C

Cuts at:      0     602    2597    2678    3234    3525 
   Size:        602    1995      81     556     291

  Fragments arranged by size: 

                1995     602     556     291      81
 
BspHI T'CATG_A

Cuts at:      0    2036    2922    3525 
   Size:       2036     886     603

  Fragments arranged by size: 

                2036     886     603
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2106    3128    3525 
   Size:       2106    1022     397

  Fragments arranged by size: 

                2106    1022     397
 
BsrDI GCAATG_nn'

Cuts at:      0     376     809    1139    2951    3525 
   Size:        376     433     330    1812     574

  Fragments arranged by size: 

                1812     574     433     376     330
 
BsrGI T'GTAC_A

Cuts at:      0    1034    1138    3525 
   Size:       1034     104    2387

  Fragments arranged by size: 

                2387    1034     104
 
BssSI C'ACGA_G

Cuts at:      0    3172    3525 
   Size:       3172     353
 
BstBI TT'CG_AA

Cuts at:      0    2403    3525 
   Size:       2403    1122
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237    3396    3525 
   Size:        237    3159     129

  Fragments arranged by size: 

                3159     237     129
 
BstYI r'GATC_y

Cuts at:      0     225     312     669     753    1275    1287    2354    2508 
   Size:        225      87     357      84     522      12    1067     154

Cuts at:   2508    3525 
   Size:       1017

  Fragments arranged by size: 

                1067    1017     522     357     225     154      87      84
                  12
 
BtgI C'CryG_G

Cuts at:      0    2690    3525 
   Size:       2690     835
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1984    3525 
   Size:       1984    1541
 
BtsI GCAGTG_nn'

Cuts at:      0     309    1230    2868    3142    3525 
   Size:        309     921    1638     274     383

  Fragments arranged by size: 

                1638     921     383     309     274
 
DraI TTT'AAA

Cuts at:      0    2795    2825    3525 
   Size:       2795      30     700

  Fragments arranged by size: 

                2795     700      30
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2302    3525 
   Size:       2302    1223
 
EaeI y'GGCC_r

Cuts at:      0       2    2346    3525 
   Size:          2    2344    1179

  Fragments arranged by size: 

                2344    1179       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     118    1487    2255    3525 
   Size:        118    1369     768    1270

  Fragments arranged by size: 

                1369    1270     768     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     300    3525 
   Size:        300    3225
 
EcoICRI GAG'CTC

Cuts at:      0    2676    3525 
   Size:       2676     849
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     390    1421    1469    3525 
   Size:        390    1031      48    2056

  Fragments arranged by size: 

                2056    1031     390      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1153    2938    3525 
   Size:       1153    1785     587

  Fragments arranged by size: 

                1785    1153     587
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1611    3525 
   Size:       1611    1914
 
EcoRI G'AATT_C

Cuts at:      0    1537    2209    3433    3525 
   Size:       1537     672    1224      92

  Fragments arranged by size: 

                1537    1224     672      92
 
EcoRV GAT'ATC

Cuts at:      0     883    1146    2386    2490    3525 
   Size:        883     263    1240     104    1035

  Fragments arranged by size: 

                1240    1035     883     263     104
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     561    2799    3436    3525 
   Size:        561    2238     637      89

  Fragments arranged by size: 

                2238     637     561      89
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102     636     668    1031    1063    1868    1900 
   Size:         70      32     534      32     363      32     805      32

Cuts at:   1900    2053    2085    2488    2520    3006    3013    3038    3045 
   Size:        153      32     403      32     486       7      25       7

Cuts at:   3045    3525 
   Size:        480

  Fragments arranged by size: 

                 805     534     486     480     403     363     153      70
                  32      32      32      32      32      32      25       7
                   7
 
HincII GTy'rAC

Cuts at:      0    2883    2918    3525 
   Size:       2883      35     607

  Fragments arranged by size: 

                2883     607      35
 
HindIII A'AGCT_T

Cuts at:      0    1424    3525 
   Size:       1424    2101
 
KpnI G_GTAC'C

Cuts at:      0    2019    3525 
   Size:       2019    1506
 
MfeI C'AATT_G

Cuts at:      0    1823    1975    3525 
   Size:       1823     152    1550

  Fragments arranged by size: 

                1823    1550     152
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     237    3525 
   Size:        237    3288
 
MscI TGG'CCA

Cuts at:      0       4    2348    3525 
   Size:          4    2344    1177

  Fragments arranged by size: 

                2344    1177       4
 
MslI CAynn'nnrTG

Cuts at:      0     817    2227    3525 
   Size:        817    1410    1298

  Fragments arranged by size: 

                1410    1298     817
 
MspA1I CmG'CkG

Cuts at:      0    1419    3192    3525 
   Size:       1419    1773     333

  Fragments arranged by size: 

                1773    1419     333
 
NdeI CA'TA_TG

Cuts at:      0    2466    3525 
   Size:       2466    1059
 
NspI r_CATG'y

Cuts at:      0     437    1776    1822    1905    2839    3525 
   Size:        437    1339      46      83     934     686

  Fragments arranged by size: 

                1339     934     686     437      83      46
 
PciI A'CATG_T

Cuts at:      0    1772    3525 
   Size:       1772    1753
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     942     954    3525 
   Size:        942      12    2571

  Fragments arranged by size: 

                2571     942      12
 
PshAI GACnn'nnGTC

Cuts at:      0    1574    3525 
   Size:       1574    1951
 
PsiI TTA'TAA

Cuts at:      0    1655    1769    1841    3525 
   Size:       1655     114      72    1684

  Fragments arranged by size: 

                1684    1655     114      72
 
PstI C_TGCA'G

Cuts at:      0     304    1237    3525 
   Size:        304     933    2288

  Fragments arranged by size: 

                2288     933     304
 
PvuII CAG'CTG

Cuts at:      0    1419    3192    3525 
   Size:       1419    1773     333

  Fragments arranged by size: 

                1773    1419     333
 
SacI G_AGCT'C

Cuts at:      0    2678    3525 
   Size:       2678     847
 
SalI G'TCGA_C

Cuts at:      0    2916    3525 
   Size:       2916     609
 
SfcI C'TryA_G

Cuts at:      0     300    1233    1622    3525 
   Size:        300     933     389    1903

  Fragments arranged by size: 

                1903     933     389     300
 
SmlI C'TyrA_G

Cuts at:      0     439     556    2307    2744    3525 
   Size:        439     117    1751     437     781

  Fragments arranged by size: 

                1751     781     439     437     117
 
SnaBI TAC'GTA

Cuts at:      0    2163    2203    3525 
   Size:       2163      40    1322

  Fragments arranged by size: 

                2163    1322      40
 
SpeI A'CTAG_T

Cuts at:      0    2575    3525 
   Size:       2575     950
 
SspI AAT'ATT

Cuts at:      0    1267    1761    2451    3525 
   Size:       1267     494     690    1074

  Fragments arranged by size: 

                1267    1074     690     494
 
StuI AGG'CCT

Cuts at:      0     383     854    3525 
   Size:        383     471    2671

  Fragments arranged by size: 

                2671     471     383
 
StyI C'CwwG_G

Cuts at:      0    1871    3525 
   Size:       1871    1654
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     194    1786    3525 
   Size:        194    1592    1739

  Fragments arranged by size: 

                1739    1592     194
 
TatI w'GTAC_w

Cuts at:      0    1034    1138    1250    3525 
   Size:       1034     104     112    2275

  Fragments arranged by size: 

                2275    1034     112     104
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     279     311    2477    2509    3525 
   Size:        279      32    2166      32    1016

  Fragments arranged by size: 

                2166    1016     279      32      32
 
XbaI T'CTAG_A

Cuts at:      0    3429    3525 
   Size:       3429      96
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2697    3525 
   Size:       2697     828
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApoI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI     BclI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I
   HincII  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NdeI     NspI     PciI    PflMI    PshAI     PsiI     PstI    PvuII
     SacI     SalI     SfcI     SmlI    SnaBI     SpeI     SspI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BbeI    BciVI    BfrBI     BlpI     BmtI     BplI    BsaBI
    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
   BstAPI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII     EagI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     MluI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SphI     SrfI
     SwaI  Tth111I     XhoI     XmaI     XmnI     ZraI
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