Restriction map for pF1KB3482
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraII       DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PvuI        RsrII       
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        SspBI       StuI        
SwaI        Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AciI         1755  CCGC                 CCGC(-3/-1)
AclI          870  AACGTT               AA^CGTT
AlwI         1824  GATCC                GGATC(4/5)
AlwNI        1387  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1368  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         386  GAGCTC               G[AT]GC[AT]^C
AspI         1058  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1191  CCCGAG               C^[CT]CG[GA]G
BalI          575  TGGCCA               TGG^CCA
BanI         2064  GGCACC               G^G[CT][GA]CC
BcnI         2084  CCGGG                CC^[GC]GG
BfaI          823  CTAG                 C^TAG
BglI         1648  GCCACAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2095  AGATCT               A^GATCT
BsaI          667  GAGACC               GGTCTC(1/5)
BsgI          332  CTGCAC               GTGCAG(16/14)
BsiHKAI       386  GAGCTC               G[AT]GC[AT]^C
BsmFI        1051  GGGAC                GGGAC(10/14)
BsmI         1803  GAATGC               GAATGC(1/-1)
BsoBI        1191  CCCGAG               C^[CT]CG[GA]G
BsrFI         481  GCCGGC               [GA]^CCGG[CT]
BsrI          622  CCAGT                ACTGG(1/-1)
BstBI         217  TTCGAA               TT^CGAA
BstXI         491  CCACAGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        481  GCCGGC               [GA]^CCGG[CT]
DrdI          522  GACCTCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          497  CCATGG               C^C[GA][CT]GG
EaeI          575  TGGCCA               [CT]^GGCC[GA]
EarI          133  GAAGAG               CTCTTC(1/4)
EcoRI        1945  GAATTC               G^AATTC
EcoT14I       497  CCATGG               C^C[AT][AT]GG
EcoT22I      1156  ATGCAT               ATGCA^T
HindIII       941  AAGCTT               A^AGCTT
Ksp632I       133  GAAGAG               CTCTTC(1/4)
MaeI          823  CTAG                 C^TAG
MluNI         575  TGGCCA               TGG^CCA
MscI          575  TGGCCA               TGG^CCA
MspA1I       1442  CAGCTG               C[AC]G^C[GT]G
NaeI          481  GCCGGC               GCC^GGC
NciI         2084  CCGGG                CC^[GC]GG
NcoI          497  CCATGG               C^CATGG
NgoMIV        481  GCCGGC               G^CCGGC
NsiI         1156  ATGCAT               ATGCA^T
NspI          485  GCATGT               [GA]CATG^[CT]
NspV          217  TTCGAA               TT^CGAA
PflFI        1058  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      870  AACGTT               AA^CGTT
PstI          473  CTGCAG               CTGCA^G
PvuII        1442  CAGCTG               CAG^CTG
SacI          386  GAGCTC               GAGCT^C
SbfI          472  CCTGCAGG             CCTGCA^GG
SfuI          217  TTCGAA               TT^CGAA
Sse8387I      472  CCTGCAGG             CCTGCA^GG
StyI          497  CCATGG               C^C[AT][AT]GG
Tth111I      1058  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1368  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BanII         386  GAGCTC               G[GA]GC[CT]^C
             1618  GAGCCC              
BbvI          347  GCAGC                GCAGC(8/12)
             1490  GCAGC               
BseRI        1295  CTCCTC               GAGGAG(10/8)
             1463  CTCCTC              
BsmAI         667  GAGAC                GTCTC(1/5)
              818  GTCTC               
BspHI        1376  TCATGA               T^CATGA
             1424  TCATGA              
BspMI         330  ACCTGC               ACCTGC(4/8)
             2050  ACCTGC              
BsrDI        1731  CATTGC               GCAATG(2/0)
             1757  GCAATG              
BstYI        1823  AGATCC               [GA]^GATC[CT]
             2095  AGATCT              
HapII         482  CCGG                 C^CGG
             2084  CCGG                
HpaII         482  CCGG                 C^CGG
             2084  CCGG                
MaeII         871  ACGT                 A^CGT
             1507  ACGT                
MflI         1823  AGATCC               [GA]^GATC[CT]
             2095  AGATCT              
MspI          482  CCGG                 C^CGG
             2084  CCGG                
RcaI         1376  TCATGA               T^CATGA
             1424  TCATGA              
SfcI          473  CTGCAG               C^T[GA][CT]AG
              704  CTACAG              
SspI          952  AATATT               AAT^ATT
             1693  AATATT              
TaqI          218  TCGA                 T^CGA
             1113  TCGA                
TseI          347  GCAGC                G^C[AT]GC
             1490  GCAGC               
TthHB8I       218  TCGA                 T^CGA
             1113  TCGA                
XhoII        1823  AGATCC               [GA]^GATC[CT]
             2095  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AvaII       
BbsI        BmyI        BpmI        BpuAI       BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
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