Restriction map for pF1KB3482
 (Linear) (Six-base) MAPSORT of: KIBB3482.seq  Check: 8984  from: 1  to: 2199



KIBB3482.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     339    2059    2199 
   Size:        339    1720     140

  Fragments arranged by size: 

                1720     339     140
 
AclI AA'CG_TT

Cuts at:      0     871    2199 
   Size:        871    1328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     872     929     937    1238    1414    1543    1556 
   Size:        383     489      57       8     301     176     129      13

Cuts at:   1556    2199 
   Size:        643

  Fragments arranged by size: 

                 643     489     383     301     176     129      57      13
                   8
 
AleI CACnn'nnGTG

Cuts at:      0    1789    2199 
   Size:       1789     410
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     160     192    2199 
   Size:        160      32    2007

  Fragments arranged by size: 

                2007     160      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1392    2199 
   Size:       1392     807
 
ApoI r'AATT_y

Cuts at:      0     146     212     766    1016    1945    2199 
   Size:        146      66     554     250     929     254

  Fragments arranged by size: 

                 929     554     254     250     146      66
 
AvaI C'yCGr_G

Cuts at:      0    1191    2199 
   Size:       1191    1008
 
BanI G'GyrC_C

Cuts at:      0    2064    2199 
   Size:       2064     135
 
BanII G_rGCy'C

Cuts at:      0     390    1622    2199 
   Size:        390    1232     577

  Fragments arranged by size: 

                1232     577     390
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245    1006    1355    1531    1559    1665    2199 
   Size:        245     761     349     176      28     106     534

  Fragments arranged by size: 

                 761     534     349     245     176     106      28
 
BfrBI ATG'CAT

Cuts at:      0    1158    2199 
   Size:       1158    1041
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1654    2199 
   Size:       1654     545
 
BglII A'GATC_T

Cuts at:      0    2095    2199 
   Size:       2095     104
 
Bme1580I G_kGCm'C

Cuts at:      0    1563    2199 
   Size:       1563     636
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     616    2199 
   Size:        616    1583
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1111    2199 
   Size:       1111    1088
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404    1081    1789    2199 
   Size:        404     677     708     410

  Fragments arranged by size: 

                 708     677     410     404
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     661    2199 
   Size:        661    1538
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     447     477    1342    1372    2199 
   Size:        447      30     865      30     827

  Fragments arranged by size: 

                 865     827     447      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1286    1454    2199 
   Size:       1286     168     745

  Fragments arranged by size: 

                1286     745     168
 
BseYI C'CCAG_C

Cuts at:      0    1440    2199 
   Size:       1440     759
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317    2199 
   Size:        317    1882
 
BsiHKAI G_wGCw'C

Cuts at:      0     390    2199 
   Size:        390    1809
 
BsmI GAATG_Cn'

Cuts at:      0    1809    2199 
   Size:       1809     390
 
Bsp1286I G_dGCh'C

Cuts at:      0     390    1563    1622    2199 
   Size:        390    1173      59     577

  Fragments arranged by size: 

                1173     577     390      59
 
BspHI T'CATG_A

Cuts at:      0    1376    1424    2199 
   Size:       1376      48     775

  Fragments arranged by size: 

                1376     775      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     339    2059    2199 
   Size:        339    1720     140

  Fragments arranged by size: 

                1720     339     140
 
BsrDI GCAATG_nn'

Cuts at:      0    1730    1764    2199 
   Size:       1730      34     435

  Fragments arranged by size: 

                1730     435      34
 
BsrFI r'CCGG_y

Cuts at:      0     481    2199 
   Size:        481    1718
 
BstBI TT'CG_AA

Cuts at:      0     218    2199 
   Size:        218    1981
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     498    2199 
   Size:        498    1701
 
BstYI r'GATC_y

Cuts at:      0    1823    2095    2199 
   Size:       1823     272     104

  Fragments arranged by size: 

                1823     272     104
 
BtgI C'CryG_G

Cuts at:      0     497    2199 
   Size:        497    1702
 
DraI TTT'AAA

Cuts at:      0     110     122     746    2199 
   Size:        110      12     624    1453

  Fragments arranged by size: 

                1453     624     110      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     528    2199 
   Size:        528    1671
 
EaeI y'GGCC_r

Cuts at:      0     575    2199 
   Size:        575    1624
 
EarI CTCTTCn'nnn_

Cuts at:      0     128    2199 
   Size:        128    2071
 
EcoICRI GAG'CTC

Cuts at:      0     388    2199 
   Size:        388    1811
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     404     872     929     937    1081    1238    1414 
   Size:        383      21     468      57       8     144     157     176

Cuts at:   1414    1543    1556    1789    2199 
   Size:        129      13     233     410

  Fragments arranged by size: 

                 468     410     383     233     176     157     144     129
                  57      21      13       8
 
EcoRI G'AATT_C

Cuts at:      0    1945    2199 
   Size:       1945     254
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      41      73     447     479    1342    1374    2080    2112 
   Size:         41      32     374      32     863      32     706      32

Cuts at:   2112    2199 
   Size:         87

  Fragments arranged by size: 

                 863     706     374      87      41      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     941    2199 
   Size:        941    1258
 
MscI TGG'CCA

Cuts at:      0     577    2199 
   Size:        577    1622
 
MslI CAynn'nnrTG

Cuts at:      0     496    1381    1789    2199 
   Size:        496     885     408     410

  Fragments arranged by size: 

                 885     496     410     408
 
MspA1I CmG'CkG

Cuts at:      0    1444    2199 
   Size:       1444     755
 
NaeI GCC'GGC

Cuts at:      0     483    2199 
   Size:        483    1716
 
NcoI C'CATG_G

Cuts at:      0     497    2199 
   Size:        497    1702
 
NgoMIV G'CCGG_C

Cuts at:      0     481    2199 
   Size:        481    1718
 
NsiI A_TGCA'T

Cuts at:      0    1160    2199 
   Size:       1160    1039
 
NspI r_CATG'y

Cuts at:      0     489    2199 
   Size:        489    1710
 
PfoI T'CCnGG_A

Cuts at:      0     381    1413    1766    2199 
   Size:        381    1032     353     433

  Fragments arranged by size: 

                1032     433     381     353
 
PstI C_TGCA'G

Cuts at:      0     477    2199 
   Size:        477    1722
 
PvuII CAG'CTG

Cuts at:      0    1444    2199 
   Size:       1444     755
 
SacI G_AGCT'C

Cuts at:      0     390    2199 
   Size:        390    1809
 
SbfI CC_TGCA'GG

Cuts at:      0     477    2199 
   Size:        477    1722
 
SfcI C'TryA_G

Cuts at:      0     473     704    2199 
   Size:        473     231    1495

  Fragments arranged by size: 

                1495     473     231
 
SspI AAT'ATT

Cuts at:      0     954    1695    2199 
   Size:        954     741     504

  Fragments arranged by size: 

                 954     741     504
 
StyI C'CwwG_G

Cuts at:      0     497    2199 
   Size:        497    1702
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     555    1826    2199 
   Size:        555    1271     373

  Fragments arranged by size: 

                1271     555     373
 
TatI w'GTAC_w

Cuts at:      0     643    2199 
   Size:        643    1556
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     713     745    1886    1918    2199 
   Size:        713      32    1141      32     281

  Fragments arranged by size: 

                1141     713     281      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1061    2199 
   Size:       1061    1138
 
XmnI GAAnn'nnTTC

Cuts at:      0    1372    2199 
   Size:       1372     827
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AleI     AloI    AlwNI     ApoI     AvaI
     BanI    BanII     BbsI    BfrBI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BstBI    BstXI
    BstYI     BtgI     DraI     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoRI    Hin4I  HindIII     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NsiI     NspI     PfoI     PstI    PvuII     SacI     SbfI
     SfcI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI     BlpI    BmgBI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     EagI     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NdeI     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     XbaI
     XcmI     XhoI     XmaI     ZraI
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