Restriction map for pF1KB3476
 (Linear) (Six-base) MAPSORT of: KIBB3476.seq  Check: 2928  from: 1  to: 2169



KIBB3476.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

Acc65I G'GTAC_C

Cuts at:      0    1661    2169 
   Size:       1661     508
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11    2169 
   Size:         11    2158
 
AflIII A'CryG_T

Cuts at:      0     166     557    1184    2169 
   Size:        166     391     627     985

  Fragments arranged by size: 

                 985     627     391     166
 
AleI CACnn'nnGTG

Cuts at:      0     467    1670    2169 
   Size:        467    1203     499

  Fragments arranged by size: 

                1203     499     467
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     485    2169 
   Size:        485    1684
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1174    1206    2169 
   Size:       1174      32     963

  Fragments arranged by size: 

                1174     963      32
 
ApoI r'AATT_y

Cuts at:      0     837     887    1072    1510    1573    1933    2164    2169 
   Size:        837      50     185     438      63     360     231       5

  Fragments arranged by size: 

                 837     438     360     231     185      63      50       5
            
 
AvaI C'yCGr_G

Cuts at:      0    1155    1358    2169 
   Size:       1155     203     811

  Fragments arranged by size: 

                1155     811     203
 
BamHI G'GATC_C

Cuts at:      0    1956    2169 
   Size:       1956     213
 
BanI G'GyrC_C

Cuts at:      0    1661    2169 
   Size:       1661     508
 
BclI T'GATC_A

Cuts at:      0    1260    2169 
   Size:       1260     909
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1296    1842    2169 
   Size:       1296     546     327

  Fragments arranged by size: 

                1296     546     327
 
BglII A'GATC_T

Cuts at:      0     774    2169 
   Size:        774    1395
 
BmgBI CAC'GTC

Cuts at:      0     169    2169 
   Size:        169    2000
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     861    2169 
   Size:        861    1308
 
BmtI G_CTAG'C

Cuts at:      0    1303    2169 
   Size:       1303     866
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1614    2169 
   Size:       1614     555
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     625     865    1358    1622    2169 
   Size:        137     488     240     493     264     547

  Fragments arranged by size: 

                 547     493     488     264     240     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     446    2169 
   Size:        446    1723
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     409    2169 
   Size:        193     216    1760

  Fragments arranged by size: 

                1760     216     193
 
BsaAI yAC'GTr

Cuts at:      0     848    1450    2169 
   Size:        848     602     719

  Fragments arranged by size: 

                 848     719     602
 
BsaBI GATnn'nnATC

Cuts at:      0    1804    1955    2169 
   Size:       1804     151     214

  Fragments arranged by size: 

                1804     214     151
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     598     628    1174    1204    2169 
   Size:        598      30     546      30     965

  Fragments arranged by size: 

                 965     598     546      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22      61    2169 
   Size:         22      39    2108

  Fragments arranged by size: 

                2108      39      22
 
BsiHKAI G_wGCw'C

Cuts at:      0     474    1344    2169 
   Size:        474     870     825

  Fragments arranged by size: 

                 870     825     474
 
BsiWI C'GTAC_G

Cuts at:      0    1450    2169 
   Size:       1450     719
 
Bsp1286I G_dGCh'C

Cuts at:      0     474    1344    2169 
   Size:        474     870     825

  Fragments arranged by size: 

                 870     825     474
 
BspHI T'CATG_A

Cuts at:      0    1005    2169 
   Size:       1005    1164
 
BsrBI CCG'CTC

Cuts at:      0    2118    2169 
   Size:       2118      51
 
BsrDI GCAATG_nn'

Cuts at:      0     594     689    2169 
   Size:        594      95    1480

  Fragments arranged by size: 

                1480     594      95
 
BsrGI T'GTAC_A

Cuts at:      0    1966    2169 
   Size:       1966     203
 
BstBI TT'CG_AA

Cuts at:      0    1986    2169 
   Size:       1986     183
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1277    1686    1938    2169 
   Size:       1277     409     252     231

  Fragments arranged by size: 

                1277     409     252     231
 
BstYI r'GATC_y

Cuts at:      0     112     774     909    1956    2169 
   Size:        112     662     135    1047     213

  Fragments arranged by size: 

                1047     662     213     135     112
 
BtgI C'CryG_G

Cuts at:      0     491    1403    2085    2169 
   Size:        491     912     682      84

  Fragments arranged by size: 

                 912     682     491      84
 
BtsI GCAGTG_nn'

Cuts at:      0     458    1245    2169 
   Size:        458     787     924

  Fragments arranged by size: 

                 924     787     458
 
DraI TTT'AAA

Cuts at:      0     824    1482    2169 
   Size:        824     658     687

  Fragments arranged by size: 

                 824     687     658
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2080    2169 
   Size:       2080      89
 
EaeI y'GGCC_r

Cuts at:      0     338    1267    2169 
   Size:        338     929     902

  Fragments arranged by size: 

                 929     902     338
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     603    2169 
   Size:         65     538    1566

  Fragments arranged by size: 

                1566     538      65
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     518    2169 
   Size:        518    1651
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     137     625     865    1358    1622    2169 
   Size:         11     126     488     240     493     264     547

  Fragments arranged by size: 

                 547     493     488     264     240     126      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     381     787    1274    2169 
   Size:        381     406     487     895

  Fragments arranged by size: 

                 895     487     406     381
 
EcoRI G'AATT_C

Cuts at:      0    1072    2169 
   Size:       1072    1097
 
EcoRV GAT'ATC

Cuts at:      0    1524    2169 
   Size:       1524     645
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     271     303    1752    1784    2169 
   Size:        271      32    1449      32     385

  Fragments arranged by size: 

                1449     385     271      32      32
 
HindIII A'AGCT_T

Cuts at:      0     641    2169 
   Size:        641    1528
 
KpnI G_GTAC'C

Cuts at:      0    1665    2169 
   Size:       1665     504
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     763    1398    2169 
   Size:        763     635     771

  Fragments arranged by size: 

                 771     763     635
 
MslI CAynn'nnrTG

Cuts at:      0     467    1670    1736    2169 
   Size:        467    1203      66     433

  Fragments arranged by size: 

                1203     467     433      66
 
MspA1I CmG'CkG

Cuts at:      0    1686    2169 
   Size:       1686     483
 
NcoI C'CATG_G

Cuts at:      0     491    1403    2169 
   Size:        491     912     766

  Fragments arranged by size: 

                 912     766     491
 
NheI G'CTAG_C

Cuts at:      0    1299    2169 
   Size:       1299     870
 
NspI r_CATG'y

Cuts at:      0     561    1188    2169 
   Size:        561     627     981

  Fragments arranged by size: 

                 981     627     561
 
PciI A'CATG_T

Cuts at:      0     557    1184    2169 
   Size:        557     627     985

  Fragments arranged by size: 

                 985     627     557
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     851    1863    2169 
   Size:        851    1012     306

  Fragments arranged by size: 

                1012     851     306
 
PfoI T'CCnGG_A

Cuts at:      0    1106    2169 
   Size:       1106    1063
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1171    1174    1203    1206    2169 
   Size:       1171       3      29       3     963

  Fragments arranged by size: 

                1171     963      29       3       3
 
PpuMI rG'GwC_Cy

Cuts at:      0     381     787    1274    2169 
   Size:        381     406     487     895

  Fragments arranged by size: 

                 895     487     406     381
 
PsiI TTA'TAA

Cuts at:      0    1058    2169 
   Size:       1058    1111
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     685     717    1853    1885    2169 
   Size:        685      32    1136      32     284

  Fragments arranged by size: 

                1136     685     284      32      32
 
PvuII CAG'CTG

Cuts at:      0    1686    2169 
   Size:       1686     483
 
RsrII CG'GwC_CG

Cuts at:      0     526    2169 
   Size:        526    1643
 
SfcI C'TryA_G

Cuts at:      0     753    1173    2122    2169 
   Size:        753     420     949      47

  Fragments arranged by size: 

                 949     753     420      47
 
SmaI CCC'GGG

Cuts at:      0    1360    2169 
   Size:       1360     809
 
SmlI C'TyrA_G

Cuts at:      0     172     424    2169 
   Size:        172     252    1745

  Fragments arranged by size: 

                1745     252     172
 
SnaBI TAC'GTA

Cuts at:      0    1450    2169 
   Size:       1450     719
 
StuI AGG'CCT

Cuts at:      0     335    2169 
   Size:        335    1834
 
StyI C'CwwG_G

Cuts at:      0     145     413     491    1270    1403    1515    2169 
   Size:        145     268      78     779     133     112     654

  Fragments arranged by size: 

                 779     654     268     145     133     112      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     472    2169 
   Size:        472    1697
 
TatI w'GTAC_w

Cuts at:      0     692    1966    2169 
   Size:        692    1274     203

  Fragments arranged by size: 

                1274     692     203
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     598     630     823     855    2169 
   Size:        598      32     193      32    1314

  Fragments arranged by size: 

                1314     598     193      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     889    2169 
   Size:        889    1280
 
XmaI C'CCGG_G

Cuts at:      0    1358    2169 
   Size:       1358     811
 
XmnI GAAnn'nnTTC

Cuts at:      0    1186    2169 
   Size:       1186     983
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     AlfI     AloI     ApoI     AvaI
    BamHI     BanI     BclI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseRI
  BsiHKAI    BsiWI Bsp1286I    BspHI    BsrBI    BsrDI    BsrGI    BstBI
    BstXI    BstYI     BtgI     BtsI     DraI     DrdI     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I  HindIII     KpnI
     MmeI     MslI   MspA1I     NcoI     NheI     NspI     PciI    PflMI
     PfoI     PpiI    PpuMI     PsiI     PsrI    PvuII    RsrII     SfcI
     SmaI     SmlI    SnaBI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BlpI
 Bme1580I     BsaI    BsaHI    BsaWI    BseYI     BsgI    BsiEI     BsmI
    BsmBI    BspEI    BspMI    BsrFI   BssHII    BssSI   BstAPI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI  EcoICRI
    EcoNI     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI    PshAI
   PspOMI    PspXI     PstI     PvuI     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     ZraI
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