Restriction map for pF1KB3435
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BbrPI       BcgI        BclI        BfrI        
BlnI        BsaAI       BsaBI       BseAI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HindIII     
HpaI        KspI        MamI        MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        469  GGTACC               G^GTACC
AlwI           23  GATCC                GGATC(4/5)
AlwNI         433  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       469  GGTACC               G^GTACC
BalI          995  TGGCCA               TGG^CCA
BanII          52  GAGCCC               G[GA]GC[CT]^C
BbeI          382  GGCGCC               GGCGC^C
BbsI         1135  GAAGAC               GAAGAC(2/6)
BbvCI         424  GCTGAGG              CCTCAGC(-5/-2)
BcnI          842  CCCGG                CC^[GC]GG
BglI          298  GCCACCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          337  GCTCAGC              GC^T[ACGT]AGC
BpmI          727  CTGGAG               CTGGAG(16/14)
Bpu1102I      337  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1135  GAAGAC               GAAGAC(2/6)
BsaI         1162  GAGACC               GGTCTC(1/5)
BsaWI         500  ACCGGA               [AT]^CCGG[AT]
BseRI         766  GAGGAG               GAGGAG(10/8)
BsiEI        1080  CGGTCG               CG[GA][CT]^CG
BsmBI         932  GAGACG               CGTCTC(1/5)
BspDI         264  ATCGAT               AT^CGAT
BspHI         680  TCATGA               T^CATGA
BstAPI        432  GCAGAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        666  GGTGACC              G^GT[ACGT]ACC
BstPI         666  GGTGACC              G^GT[ACGT]ACC
CelII         337  GCTCAGC              GC^T[ACGT]AGC
CfoI          383  GCGC                 GCG^C
ClaI          264  ATCGAT               AT^CGAT
DraII         686  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        785  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      686  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       666  GGTGACC              G^GT[ACGT]ACC
EcoT14I       308  CCTTGG               C^C[AT][AT]GG
HaeII         382  GGCGCC               [GA]GCGC^[CT]
HgaI           62  GCGTC                GACGC(5/10)
HhaI          383  GCGC                 GCG^C
HinP1I        383  GCGC                 G^CGC
KasI          382  GGCGCC               G^GCGCC
KpnI          469  GGTACC               GGTAC^C
MluNI         995  TGGCCA               TGG^CCA
MscI          995  TGGCCA               TGG^CCA
NarI          382  GGCGCC               GG^CGCC
NciI          842  CCCGG                CC^[GC]GG
NspI          876  GCATGC               [GA]CATG^[CT]
PflMI         303  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         686  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          680  TCATGA               T^CATGA
SfoI          382  GGCGCC               GGC^GCC
SphI          876  GCATGC               GCATG^C
StuI          305  AGGCCT               AGG^CCT
StyI          308  CCTTGG               C^C[AT][AT]GG
Van91I        303  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AciI          738  CCGC                 CCGC(-3/-1)
              899  CCGC                
AcyI           61  GGCGTC               G[GA]^CG[CT]C
              382  GGCGCC              
AfaI          470  GTAC                 GT^AC
              538  GTAC                
AvaI          326  CTCGAG               C^[CT]CG[GA]G
              881  CTCGAG              
BciVI         734  GTATCC               GTATCC(6/5)
              895  GTATCC              
BglII         288  AGATCT               A^GATCT
              584  AGATCT              
BmyI           52  GAGCCC               G[AGT]GC[ACT]^C
              408  GGGCAC              
BsaHI          61  GGCGTC               G[GA]^CG[CT]C
              382  GGCGCC              
BsgI          430  GTGCAG               GTGCAG(16/14)
             1012  GTGCAG              
BsoBI         326  CTCGAG               C^[CT]CG[GA]G
              881  CTCGAG              
Bsp1286I       52  GAGCCC               G[AGT]GC[ACT]^C
              408  GGGCAC              
BspMI         989  ACCTGC               ACCTGC(4/8)
             1017  GCAGGT              
BstYI         288  AGATCT               [GA]^GATC[CT]
              584  AGATCT              
DdeI          338  CTCAG                C^T[ACGT]AG
              425  CTGAG               
DsaI          132  CCGTGG               C^C[GA][CT]GG
              567  CCGTGG              
EarI         1148  CTCTTC               CTCTTC(1/4)
              715  GAAGAG              
EcoT22I       874  ATGCAT               ATGCA^T
              904  ATGCAT              
HapII         501  CCGG                 C^CGG
              843  CCGG                
Hin1I          61  GGCGTC               G[GA]^CG[CT]C
              382  GGCGCC              
HincII         91  GTTGAC               GT[CT]^[GA]AC
              720  GTTGAC              
HindII         91  GTTGAC               GT[CT]^[GA]AC
              720  GTTGAC              
HpaII         501  CCGG                 C^CGG
              843  CCGG                
Ksp632I      1148  CTCTTC               CTCTTC(1/4)
              715  GAAGAG              
MflI          288  AGATCT               [GA]^GATC[CT]
              584  AGATCT              
MspI          501  CCGG                 C^CGG
              843  CCGG                
NsiI          874  ATGCAT               ATGCA^T
              904  ATGCAT              
PaeR7I        326  CTCGAG               C^TCGAG
              881  CTCGAG              
PleI         1146  GACTC                GAGTC(4/5)
              121  GAGTC               
PstI          271  CTGCAG               CTGCA^G
              439  CTGCAG              
RsaI          470  GTAC                 GT^AC
              538  GTAC                
SfaNI         873  GATGC                GCATC(5/9)
              137  GCATC               
SfcI          271  CTGCAG               C^T[GA][CT]AG
              439  CTGCAG              
Tsp509I       284  AATT                 ^AATT
              332  AATT                
TspRI         891  CACTG                CA[GC]TG[ACGT][ACGT]^
              984  CACTG               
XhoI          326  CTCGAG               C^TCGAG
              881  CTCGAG              
XhoII         288  AGATCT               [GA]^GATC[CT]
              584  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AluI        AvaII       BanI        BbvI        BfaI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DpnII       EaeI        EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TthHB8I     XcmI        
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