Restriction map for pF1KB3347
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

Acc65I      AccI        AccIII      AcsI        AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspLU11I    BspMI       
BsrBI       Bst1107I    BstBI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       FseI        FspI        HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII        1240  GACGTC               GACGT^C
AccII         496  CGCG                 CG^CG
AclI          117  AACGTT               AA^CGTT
AcyI         1240  GACGTC               G[GA]^CG[CT]C
AflII          27  CTTAAG               C^TTAAG
AocI         1138  CCTCAGG              CC^T[ACGT]AGG
ApaI          860  GGGCCC               GGGCC^C
AspI          868  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1574  GGACC                G^G[AT]CC
BalI         1286  TGGCCA               TGG^CCA
BciVI        1557  GGATAC               GTATCC(6/5)
BclI          941  TGATCA               T^GATCA
BfrI           27  CTTAAG               C^TTAAG
BsaHI        1240  GACGTC               G[GA]^CG[CT]C
BsaWI        1026  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     1982  TGTACA               T^GTACA
BspHI        2205  TCATGA               T^CATGA
BsrFI          32  GCCGGC               [GA]^CCGG[CT]
BsrGI        1982  TGTACA               T^GTACA
BssHII        495  GCGCGC               G^CGCGC
BssSI        1563  CACGAG               CACGAG(-5/-1)
BstAPI         94  GCATTTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         496  CGCG                 CG^CG
Bsu36I       1138  CCTCAGG              CC^T[ACGT]AGG
Cfr10I         32  GCCGGC               [GA]^CCGG[CT]
DraII         859  AGGGCCC              [GA]G^G[ACGT]CC[CT]
Eco81I       1138  CCTCAGG              CC^T[ACGT]AGG
EcoNI         256  CCTTTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      859  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      1842  ATGCAT               ATGCA^T
FbaI          941  TGATCA               T^GATCA
HaeII         704  AGCGCC               [GA]GCGC^[CT]
HgaI         1221  GCGTC                GACGC(5/10)
Hin1I        1240  GACGTC               G[GA]^CG[CT]C
HincII         55  GTTGAC               GT[CT]^[GA]AC
HindII         55  GTTGAC               GT[CT]^[GA]AC
HindIII       634  AAGCTT               A^AGCTT
MluNI        1286  TGGCCA               TGG^CCA
MscI         1286  TGGCCA               TGG^CCA
MvnI          496  CGCG                 CG^CG
NaeI           32  GCCGGC               GCC^GGC
NcoI         1005  CCATGG               C^CATGG
NgoMIV         32  GCCGGC               G^CCGGC
NsiI         1842  ATGCAT               ATGCA^T
PaeR7I        932  CTCGAG               C^TCGAG
PflFI         868  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         663  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      117  AACGTT               AA^CGTT
RcaI         2205  TCATGA               T^CATGA
SacI          929  GAGCTC               GAGCT^C
SapI         1951  GAAGAGC              GCTCTTC(1/4)
ScaI         1945  AGTACT               AGT^ACT
SmaI          500  CCCGGG               CCC^GGG
SspBI        1982  TGTACA               T^GTACA
StuI          436  AGGCCT               AGG^CCT
TaqI          933  TCGA                 T^CGA
Tth111I       868  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       933  TCGA                 T^CGA
Van91I        663  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          932  CTCGAG               C^TCGAG
XmaCI         500  CCCGGG               C^CCGGG
XmaI          500  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BcnI          500  CCCGG                CC^[GC]GG
              501  CCGGG               
BpmI         1156  CTCCAG               CTGGAG(16/14)
              727  CTGGAG              
BsaBI         457  GATATGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2101  GATGTTGATC          
BstEII        814  GGTAACC              G^GT[ACGT]ACC
             1938  GGTCACC             
BstF5I        156  GGATG                GGATG(2/0)
              852  GGATG               
BstPI         814  GGTAACC              G^GT[ACGT]ACC
             1938  GGTCACC             
BstYI         845  AGATCC               [GA]^GATC[CT]
             1160  AGATCC              
DrdI         1093  GACACTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1575  GACCAAGATGTC        
DsaI         1005  CCATGG               C^C[GA][CT]GG
             1415  CCACGG              
EcoO65I       814  GGTAACC              G^GT[ACGT]ACC
             1938  GGTCACC             
EcoT14I       141  CCAAGG               C^C[AT][AT]GG
             1005  CCATGG              
FokI          156  GGATG                GGATG(9/13)
              852  GGATG               
MamI          457  GATATGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2101  GATGTTGATC          
MflI          845  AGATCC               [GA]^GATC[CT]
             1160  AGATCC              
NciI          500  CCCGG                CC^[GC]GG
              501  CCGGG               
PstI          757  CTGCAG               CTGCA^G
             1225  CTGCAG              
SfcI          757  CTGCAG               C^T[GA][CT]AG
             1225  CTGCAG              
SmlI           27  CTTAAG               C^T[CT][GA]AG
              932  CTCGAG              
SphI          180  GCATGC               GCATG^C
              833  GCATGC              
StyI          141  CCAAGG               C^C[AT][AT]GG
             1005  CCATGG              
Tsp45I       1740  GTCAC                ^GT[GC]AC
             1939  GTCAC               
XhoII         845  AGATCC               [GA]^GATC[CT]
             1160  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        BanII       BbsI        BbvI        
BfaI        BmyI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EarI        
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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