Restriction map for pF1KB3307
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BciVI       BclI        BfrI        BglII       BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspHI       BspMI       BsrDI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoT22I     FbaI        FspI        HaeII       Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SmaI        SnaBI       SphI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI           75  GTCGAC               GT^[AC][GT]AC
AccIII        584  TCCGGA               T^CCGGA
AflIII       1121  ACATGT               A^C[GA][CT]GT
Alw44I       1624  GTGCAC               G^TGCAC
AocI         2089  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1624  GTGCAC               G^TGCAC
AvaI         2054  CTCGAG               C^[CT]CG[GA]G
AvrII         438  CCTAGG               C^CTAGG
BlnI          438  CCTAGG               C^CTAGG
BsaWI         584  TCCGGA               [AT]^CCGG[AT]
BseAI         584  TCCGGA               T^CCGGA
BsoBI        2054  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1387  TGTACA               T^GTACA
BspEI         584  TCCGGA               T^CCGGA
BspLU11I     1121  ACATGT               A^CATGT
BsrGI        1387  TGTACA               T^GTACA
Bsu36I       2089  CCTGAGG              CC^T[ACGT]AGG
CfoI          909  GCGC                 GCG^C
EarI          931  CTCTTC               CTCTTC(1/4)
Eco81I       2089  CCTGAGG              CC^T[ACGT]AGG
EcoRV         186  GATATC               GAT^ATC
FseI         1645  GGCCGGCC             GGCCGG^CC
HhaI          909  GCGC                 GCG^C
HinP1I        909  GCGC                 G^CGC
HindIII       643  AAGCTT               A^AGCTT
Ksp632I       931  CTCTTC               CTCTTC(1/4)
MaeII        1622  ACGT                 A^CGT
MroI          584  TCCGGA               T^CCGGA
NaeI         1646  GCCGGC               GCC^GGC
NgoMIV       1646  GCCGGC               G^CCGGC
NspI         1121  ACATGT               [GA]CATG^[CT]
PaeR7I       2054  CTCGAG               C^TCGAG
SacI          928  GAGCTC               GAGCT^C
SalI           75  GTCGAC               G^TCGAC
SapI          930  GCTCTTC              GCTCTTC(1/4)
SgrAI         904  CACCGGCG             C[GA]^CCGG[CT]G
SpeI         1002  ACTAGT               A^CTAGT
SspBI        1387  TGTACA               T^GTACA
StuI         1153  AGGCCT               AGG^CCT
XcmI          903  CCACCGGCGCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2054  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI         1561  GAATTT               [GA]^AATT[CT]
             2147  AAATTT              
AfaI          467  GTAC                 GT^AC
             1388  GTAC                
ApoI         1561  GAATTT               [GA]^AATT[CT]
             2147  AAATTT              
BalI         1819  TGGCCA               TGG^CCA
             2013  TGGCCA              
BanI         1597  GGTGCC               G^G[CT][GA]CC
             1726  GGTGCC              
BbsI         1117  GAAGAC               GAAGAC(2/6)
              795  GTCTTC              
BbvCI        1956  CCTCAGC              CCTCAGC(-5/-2)
              858  GCTGAGG             
BcgI         1348  CGACTGCGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               67  GCAGTTGTGTCG        
BglI         1821  GCCACTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1885  GCCAAAGAGGC         
BpuAI        1117  GAAGAC               GAAGAC(2/6)
              795  GTCTTC              
BseRI         118  GAGGAG               GAGGAG(10/8)
              658  GAGGAG              
BssSI         977  CACGAG               CACGAG(-5/-1)
             2124  CACGAG              
BstYI        1261  AGATCC               [GA]^GATC[CT]
             1412  AGATCC              
HgaI         1372  GACGC                GACGC(5/10)
             1737  GCGTC               
HincII         75  GTCGAC               GT[CT]^[GA]AC
              709  GTTGAC              
HindII         75  GTCGAC               GT[CT]^[GA]AC
              709  GTTGAC              
HphI         1050  GGTGA                GGTGA(8/7)
             1752  TCACC               
MflI         1261  AGATCC               [GA]^GATC[CT]
             1412  AGATCC              
MluNI        1819  TGGCCA               TGG^CCA
             2013  TGGCCA              
MscI         1819  TGGCCA               TGG^CCA
             2013  TGGCCA              
PleI         1942  GACTC                GAGTC(4/5)
             2052  GACTC               
RsaI          467  GTAC                 GT^AC
             1388  GTAC                
XhoII        1261  AGATCC               [GA]^GATC[CT]
             1412  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
AspHI       AvaII       BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PpuMI       PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      
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