Restriction map for pF1KB3307
 (Linear) (Six-base) MAPSORT of: KIBB3307.seq  Check: 9957  from: 1  to: 2154



KIBB3307.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AccI GT'mk_AC

Cuts at:      0      76    2154 
   Size:         76    2078
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     782    1576    2154 
   Size:        781       1     794     578

  Fragments arranged by size: 

                 794     781     578       1
 
AflIII A'CryG_T

Cuts at:      0    1121    2154 
   Size:       1121    1033
 
AleI CACnn'nnGTG

Cuts at:      0    1656    2154 
   Size:       1656     498
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1742    1821    2154 
   Size:       1742      79     333

  Fragments arranged by size: 

                1742     333      79
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     766     798    2154 
   Size:        766      32    1356

  Fragments arranged by size: 

                1356     766      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     280     289     343     427     571    1147    1265    1554 
   Size:        280       9      54      84     144     576     118     289

Cuts at:   1554    1905    1945    2106    2154 
   Size:        351      40     161      48

  Fragments arranged by size: 

                 576     351     289     280     161     144     118      84
                  54      48      40       9
 
ApaI G_GGCC'C

Cuts at:      0     727     807    1980    2154 
   Size:        727      80    1173     174

  Fragments arranged by size: 

                1173     727     174      80
 
ApaLI G'TGCA_C

Cuts at:      0    1624    2154 
   Size:       1624     530
 
ApoI r'AATT_y

Cuts at:      0    1561    2147    2154 
   Size:       1561     586       7

  Fragments arranged by size: 

                1561     586       7
 
AvaI C'yCGr_G

Cuts at:      0    2054    2154 
   Size:       2054     100
 
AvrII C'CTAG_G

Cuts at:      0     438    2154 
   Size:        438    1716
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     269     302    2154 
   Size:        269      33    1852

  Fragments arranged by size: 

                1852     269      33
 
BanI G'GyrC_C

Cuts at:      0    1597    1726    2154 
   Size:       1597     129     428

  Fragments arranged by size: 

                1597     428     129
 
BanII G_rGCy'C

Cuts at:      0     526     727     807     932     955    1305    1980    2154 
   Size:        526     201      80     125      23     350     675     174

  Fragments arranged by size: 

                 675     526     350     201     174     125      80      23
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     788    1124    2154 
   Size:        788     336    1030

  Fragments arranged by size: 

                1030     788     336
 
BbvCI CC'TCA_GC

Cuts at:      0     859    1957    2154 
   Size:        859    1098     197

  Fragments arranged by size: 

                1098     859     197
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      56      90    1337    1371    2154 
   Size:         56      34    1247      34     783

  Fragments arranged by size: 

                1247     783      56      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1827    1891    2154 
   Size:       1827      64     263

  Fragments arranged by size: 

                1827     263      64
 
Bme1580I G_kGCm'C

Cuts at:      0     727     807    1100    1628    1731    1980    2154 
   Size:        727      80     293     528     103     249     174

  Fragments arranged by size: 

                 727     528     293     249     174     103      80
 
BmgBI CAC'GTC

Cuts at:      0    1623    2154 
   Size:       1623     531
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1110    2154 
   Size:       1110    1044
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1780    2154 
   Size:       1780     374
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     340     384    1610    1690    1753    2154 
   Size:        218     122      44    1226      80      63     401

  Fragments arranged by size: 

                1226     401     218     122      80      63      44
 
Bpu10I CC'TnA_GC

Cuts at:      0     134     859    1957    2154 
   Size:        134     725    1098     197

  Fragments arranged by size: 

                1098     725     197     134
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278    1195    2154 
   Size:        278     917     959

  Fragments arranged by size: 

                 959     917     278
 
BsaWI w'CCGG_w

Cuts at:      0     584    2154 
   Size:        584    1570
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     133     673    2154 
   Size:        133     540    1481

  Fragments arranged by size: 

                1481     540     133
 
BseYI C'CCAG_C

Cuts at:      0    1159    1979    2154 
   Size:       1159     820     175

  Fragments arranged by size: 

                1159     820     175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1153    1214    2119    2154 
   Size:       1153      61     905      35

  Fragments arranged by size: 

                1153     905      61      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     932    1628    1766    2154 
   Size:        932     696     138     388

  Fragments arranged by size: 

                 932     696     388     138
 
Bsp1286I G_dGCh'C

Cuts at:      0     526     727     807     932     955    1100    1305    1628 
   Size:        526     201      80     125      23     145     205     323

Cuts at:   1628    1731    1766    1980    2154 
   Size:        103      35     214     174

  Fragments arranged by size: 

                 526     323     214     205     201     174     145     125
                 103      80      35      23
 
BspEI T'CCGG_A

Cuts at:      0     584    2154 
   Size:        584    1570
 
BsrBI CCG'CTC

Cuts at:      0     102     324     546    2154 
   Size:        102     222     222    1608

  Fragments arranged by size: 

                1608     222     222     102
 
BsrFI r'CCGG_y

Cuts at:      0     905    1253    1646    2154 
   Size:        905     348     393     508

  Fragments arranged by size: 

                 905     508     393     348
 
BsrGI T'GTAC_A

Cuts at:      0    1387    2154 
   Size:       1387     767
 
BssSI C'ACGA_G

Cuts at:      0     977    2124    2154 
   Size:        977    1147      30

  Fragments arranged by size: 

                1147     977      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     280     289    1905    2154 
   Size:        280       9    1616     249

  Fragments arranged by size: 

                1616     280     249       9
 
BstYI r'GATC_y

Cuts at:      0    1261    1412    2154 
   Size:       1261     151     742

  Fragments arranged by size: 

                1261     742     151
 
Bsu36I CC'TnA_GG

Cuts at:      0    2090    2154 
   Size:       2090      64
 
BtsI GCAGTG_nn'

Cuts at:      0     290     392     814    1233    1766    2154 
   Size:        290     102     422     419     533     388

  Fragments arranged by size: 

                 533     422     419     388     290     102
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      74     109    2154 
   Size:         74      35    2045

  Fragments arranged by size: 

                2045      74      35
 
EaeI y'GGCC_r

Cuts at:      0    1489    1648    1819    2013    2154 
   Size:       1489     159     171     194     141

  Fragments arranged by size: 

                1489     194     171     159     141
 
EarI CTCTTCn'nnn_

Cuts at:      0     937    2154 
   Size:        937    1217
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1459    1960    2154 
   Size:       1459     501     194

  Fragments arranged by size: 

                1459     501     194
 
EcoICRI GAG'CTC

Cuts at:      0     930    2154 
   Size:        930    1224
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     340     384     781     782    1576    1610    1690 
   Size:        218     122      44     397       1     794      34      80

Cuts at:   1690    1753    2154 
   Size:         63     401

  Fragments arranged by size: 

                 794     401     397     218     122      80      63      44
                  34       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     502     585     718     789    1546    2154 
   Size:        115     387      83     133      71     757     608

  Fragments arranged by size: 

                 757     608     387     133     115      83      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     723     724     803     804    1509    1539    1551    1578 
   Size:        723       1      79       1     705      30      12      27

Cuts at:   1578    2130    2154 
   Size:        552      24

  Fragments arranged by size: 

                 723     705     552      79      30      27      24      12
                   1       1
 
EcoRV GAT'ATC

Cuts at:      0     188    2154 
   Size:        188    1966
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     666    2154 
   Size:        666    1488
 
FseI GG_CCGG'CC

Cuts at:      0    1650    2154 
   Size:       1650     504
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1570    1602    1688    1720    1927    1940    1959    1972 
   Size:       1570      32      86      32     207      13      19      13

Cuts at:   1972    2154 
   Size:        182

  Fragments arranged by size: 

                1570     207     182      86      32      32      19      13
                  13
 
HincII GTy'rAC

Cuts at:      0      77     711    2154 
   Size:         77     634    1443

  Fragments arranged by size: 

                1443     634      77
 
HindIII A'AGCT_T

Cuts at:      0     643    2154 
   Size:        643    1511
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     700    1707    2154 
   Size:        700    1007     447

  Fragments arranged by size: 

                1007     700     447
 
MscI TGG'CCA

Cuts at:      0    1821    2015    2154 
   Size:       1821     194     139

  Fragments arranged by size: 

                1821     194     139
 
MslI CAynn'nnrTG

Cuts at:      0     786    1656    1658    1858    2154 
   Size:        786     870       2     200     296

  Fragments arranged by size: 

                 870     786     296     200       2
 
MspA1I CmG'CkG

Cuts at:      0     427     568    1401    2154 
   Size:        427     141     833     753

  Fragments arranged by size: 

                 833     753     427     141
 
NaeI GCC'GGC

Cuts at:      0    1648    2154 
   Size:       1648     506
 
NgoMIV G'CCGG_C

Cuts at:      0    1646    2154 
   Size:       1646     508
 
NspI r_CATG'y

Cuts at:      0    1125    2154 
   Size:       1125    1029
 
PasI CC'CwG_GG

Cuts at:      0     955    1574    1641    2154 
   Size:        955     619      67     513

  Fragments arranged by size: 

                 955     619     513      67
 
PciI A'CATG_T

Cuts at:      0    1121    2154 
   Size:       1121    1033
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     414    1265    1848    2154 
   Size:        414     851     583     306

  Fragments arranged by size: 

                 851     583     414     306
 
PfoI T'CCnGG_A

Cuts at:      0     195    2154 
   Size:        195    1959
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     914     946    2154 
   Size:        914      32    1208

  Fragments arranged by size: 

                1208     914      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1509    1539    1578    2130    2154 
   Size:       1509      30      39     552      24

  Fragments arranged by size: 

                1509     552      39      30      24
 
PspOMI G'GGCC_C

Cuts at:      0     723     803    1976    2154 
   Size:        723      80    1173     178

  Fragments arranged by size: 

                1173     723     178      80
 
PstI C_TGCA'G

Cuts at:      0     285     457     821    1917    2154 
   Size:        285     172     364    1096     237

  Fragments arranged by size: 

                1096     364     285     237     172
 
PvuII CAG'CTG

Cuts at:      0     427     568    1401    2154 
   Size:        427     141     833     753

  Fragments arranged by size: 

                 833     753     427     141
 
SacI G_AGCT'C

Cuts at:      0     932    2154 
   Size:        932    1222
 
SalI G'TCGA_C

Cuts at:      0      75    2154 
   Size:         75    2079
 
SanDI GG'GwC_CC

Cuts at:      0    1509    2130    2154 
   Size:       1509     621      24

  Fragments arranged by size: 

                1509     621      24
 
SapI GCTCTTCn'nnn_

Cuts at:      0     937    2154 
   Size:        937    1217
 
SfcI C'TryA_G

Cuts at:      0     281     453     817    1913    2116    2154 
   Size:        281     172     364    1096     203      38

  Fragments arranged by size: 

                1096     364     281     203     172      38
 
SgrAI Cr'CCGG_yG

Cuts at:      0     905    2154 
   Size:        905    1249
 
SmlI C'TyrA_G

Cuts at:      0     293    1210    2054    2154 
   Size:        293     917     844     100

  Fragments arranged by size: 

                 917     844     293     100
 
SpeI A'CTAG_T

Cuts at:      0    1002    2154 
   Size:       1002    1152
 
StuI AGG'CCT

Cuts at:      0    1155    2154 
   Size:       1155     999
 
StyI C'CwwG_G

Cuts at:      0      36     329     438    2070    2154 
   Size:         36     293     109    1632      84

  Fragments arranged by size: 

                1632     293     109      84      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     421     683    2154 
   Size:        421     262    1471

  Fragments arranged by size: 

                1471     421     262
 
TatI w'GTAC_w

Cuts at:      0     466    1387    2154 
   Size:        466     921     767

  Fragments arranged by size: 

                 921     767     466
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     910    2154 
   Size:        910    1244
 
XhoI C'TCGA_G

Cuts at:      0    2054    2154 
   Size:       2054     100
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    AvrII     BaeI     BanI    BanII     BbsI
    BbvCI     BcgI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI    BstYI   Bsu36I
     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FseI    Hin4I   HincII  HindIII     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
     SalI    SanDI     SapI     SfcI    SgrAI     SmlI     SpeI     StuI
     StyI    TaqII     TatI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AscI     AseI    AsiSI    BamHI     BbeI    BciVI     BclI    BfrBI
    BglII     BlpI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrDI   BssHII
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI    EcoRI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI     SmaI    SnaBI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com