Restriction map for
pF1KB3247
(Linear) (Six-base) MAPSORT of: KIBB3247.seq Check: 6466 from: 1 to: 1083
KIBB3247.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 82 1083
Size: 82 1001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 395 515 551 1083
Size: 347 48 120 36 532
Fragments arranged by size:
532 347 120 48 36
AleI CACnn'nnGTG
Cuts at: 0 307 689 1083
Size: 307 382 394
Fragments arranged by size:
394 382 307
AlwNI CAG_nnn'CTG
Cuts at: 0 629 1083
Size: 629 454
ApoI r'AATT_y
Cuts at: 0 794 1083
Size: 794 289
BamHI G'GATC_C
Cuts at: 0 269 1083
Size: 269 814
BanI G'GyrC_C
Cuts at: 0 454 1083
Size: 454 629
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 626 1083
Size: 503 123 457
Fragments arranged by size:
503 457 123
BclI T'GATC_A
Cuts at: 0 809 905 1083
Size: 809 96 178
Fragments arranged by size:
809 178 96
BlpI GC'TnA_GC
Cuts at: 0 49 1083
Size: 49 1034
Bme1580I G_kGCm'C
Cuts at: 0 254 1083
Size: 254 829
BmgBI CAC'GTC
Cuts at: 0 531 1083
Size: 531 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 683 1083
Size: 330 353 400
Fragments arranged by size:
400 353 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1001 1083
Size: 531 470 82
Fragments arranged by size:
531 470 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 662 1083
Size: 217 445 421
Fragments arranged by size:
445 421 217
BsaAI yAC'GTr
Cuts at: 0 105 255 1083
Size: 105 150 828
Fragments arranged by size:
828 150 105
BsaBI GATnn'nnATC
Cuts at: 0 720 1083
Size: 720 363
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 1083
Size: 265 30 788
Fragments arranged by size:
788 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 534 1083
Size: 534 549
BseYI C'CCAG_C
Cuts at: 0 508 1083
Size: 508 575
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1083
Size: 311 772
Bsp1286I G_dGCh'C
Cuts at: 0 254 1083
Size: 254 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 88 221 235 410 1061 1083
Size: 82 6 133 14 175 651 22
Fragments arranged by size:
651 175 133 82 22 14 6
BsrDI GCAATG_nn'
Cuts at: 0 857 924 1083
Size: 857 67 159
Fragments arranged by size:
857 159 67
BsrGI T'GTAC_A
Cuts at: 0 311 385 600 1083
Size: 311 74 215 483
Fragments arranged by size:
483 311 215 74
BssSI C'ACGA_G
Cuts at: 0 40 1083
Size: 40 1043
BstXI CCAn_nnnn'nTGG
Cuts at: 0 691 1083
Size: 691 392
BstYI r'GATC_y
Cuts at: 0 269 715 1083
Size: 269 446 368
Fragments arranged by size:
446 368 269
ClaI AT'CG_AT
Cuts at: 0 65 1083
Size: 65 1018
DraIII CAC_nnn'GTG
Cuts at: 0 258 1083
Size: 258 825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 395 515 531 551 1001 1083
Size: 347 48 120 16 20 450 82
Fragments arranged by size:
450 347 120 82 48 20 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 1083
Size: 157 926
EcoO109I rG'GnC_Cy
Cuts at: 0 698 1083
Size: 698 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 373 405 563 595 718 750
Size: 23 32 318 32 158 32 123 32
Cuts at: 750 1083
Size: 333
Fragments arranged by size:
333 318 158 123 32 32 32 32
23
HincII GTy'rAC
Cuts at: 0 444 867 1083
Size: 444 423 216
Fragments arranged by size:
444 423 216
HpaI GTT'AAC
Cuts at: 0 444 1083
Size: 444 639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1083
Size: 350 733
MslI CAynn'nnrTG
Cuts at: 0 307 689 737 1083
Size: 307 382 48 346
Fragments arranged by size:
382 346 307 48
MspA1I CmG'CkG
Cuts at: 0 48 148 539 1036 1083
Size: 48 100 391 497 47
Fragments arranged by size:
497 391 100 48 47
PmlI CAC'GTG
Cuts at: 0 105 255 1083
Size: 105 150 828
Fragments arranged by size:
828 150 105
PpuMI rG'GwC_Cy
Cuts at: 0 698 1083
Size: 698 385
PstI C_TGCA'G
Cuts at: 0 232 1058 1083
Size: 232 826 25
Fragments arranged by size:
826 232 25
PvuII CAG'CTG
Cuts at: 0 1036 1083
Size: 1036 47
SanDI GG'GwC_CC
Cuts at: 0 698 1083
Size: 698 385
SbfI CC_TGCA'GG
Cuts at: 0 232 1083
Size: 232 851
SfcI C'TryA_G
Cuts at: 0 123 228 1054 1083
Size: 123 105 826 29
Fragments arranged by size:
826 123 105 29
StyI C'CwwG_G
Cuts at: 0 833 1083
Size: 833 250
TatI w'GTAC_w
Cuts at: 0 311 385 600 1083
Size: 311 74 215 483
Fragments arranged by size:
483 311 215 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1083
Size: 265 32 786
Fragments arranged by size:
786 265 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 709 1083
Size: 709 374
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI BamHI BanI BbsI
BclI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI Bsp1286I BspMI BsrDI
BsrGI BssSI BstXI BstYI ClaI DraIII Eco57MI EcoNI
EcoO109I Hin4I HincII HpaI MmeI MslI MspA1I PmlI
PpuMI PstI PvuII SanDI SbfI SfcI StyI TatI
TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BmtI BplI Bpu10I BpuEI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI ZraI