(Circular) (Six-base) MAPSORT of: pF1KB3114.seq Check: 6704 from: 1 to: 4748 pF1KB3114 4748 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 257 1786 257 Size: 1529 3219 Acc65I G'GTAC_C Cuts at: 1764 1764 Size: 4748 AclI AA'CG_TT Cuts at: 4588 4588 Size: 4748 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 359 401 655 1175 1618 1736 2573 3005 3358 Size: 42 254 520 443 118 837 432 353 Cuts at: 3358 4020 359 Size: 662 1087 Fragments arranged by size: 1087 837 662 520 443 432 353 254 118 42 AflIII A'CryG_T Cuts at: 1100 2100 3900 4181 1100 Size: 1000 1800 281 1667 Fragments arranged by size: 1800 1667 1000 281 AleI CACnn'nnGTG Cuts at: 986 1495 986 Size: 509 4239 AlwNI CAG_nnn'CTG Cuts at: 198 553 2053 3491 198 Size: 355 1500 1438 1455 Fragments arranged by size: 1500 1455 1438 355 ApaLI G'TGCA_C Cuts at: 3586 3586 Size: 4748 ApoI r'AATT_y Cuts at: 457 491 851 1035 1752 4101 4357 457 Size: 34 360 184 717 2349 256 848 Fragments arranged by size: 2349 848 717 360 256 184 34 AseI AT'TA_AT Cuts at: 20 20 Size: 4748 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4748 AvaI C'yCGr_G Cuts at: 1768 1768 Size: 4748 BamHI G'GATC_C Cuts at: 1773 1773 Size: 4748 BanI G'GyrC_C Cuts at: 1764 2178 2426 2461 1764 Size: 414 248 35 4051 Fragments arranged by size: 4051 414 248 35 BanII G_rGCy'C Cuts at: 350 1762 2792 350 Size: 1412 1030 2306 Fragments arranged by size: 2306 1412 1030 BbeI G_GCGC'C Cuts at: 2182 2430 2182 Size: 248 4500 BbsI GAAGACnn'nnnn_ Cuts at: 330 1706 330 Size: 1376 3372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 583 617 583 Size: 34 4714 BciVI GTATCCnnnnn_n' Cuts at: 327 2646 3702 4748 327 Size: 2319 1056 1046 327 Fragments arranged by size: 2319 1056 1046 327 BclI T'GATC_A Cuts at: 2268 2268 Size: 4748 BglII A'GATC_T Cuts at: 2263 4300 2263 Size: 2037 2711 BlpI GC'TnA_GC Cuts at: 1860 1860 Size: 4748 Bme1580I G_kGCm'C Cuts at: 2373 2466 3590 2373 Size: 93 1124 3531 Fragments arranged by size: 3531 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 1344 1960 2041 2376 4520 1344 Size: 616 81 335 2144 1572 Fragments arranged by size: 2144 1572 616 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1464 3115 1464 Size: 1651 3097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1074 1074 Size: 4748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 991 1924 2803 3517 3815 991 Size: 933 879 714 298 1924 Fragments arranged by size: 1924 933 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 4452 4452 Size: 4748 BsaAI yAC'GTr Cuts at: 984 2101 984 Size: 1117 3631 BsaBI GATnn'nnATC Cuts at: 6 2262 4299 6 Size: 2256 2037 455 Fragments arranged by size: 2256 2037 455 BsaHI Gr'CG_yC Cuts at: 2179 2427 3129 4625 2179 Size: 248 702 1496 2302 Fragments arranged by size: 2302 1496 702 248 BsaWI w'CCGG_w Cuts at: 1937 2458 3136 3547 3694 3968 1937 Size: 521 678 411 147 274 2717 Fragments arranged by size: 2717 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1279 1279 Size: 4748 BseYI C'CCAG_C Cuts at: 1215 2173 2931 3596 4004 1215 Size: 958 758 665 408 1959 Fragments arranged by size: 1959 958 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1010 1649 1010 Size: 639 4109 BsiEI CG_ry'CG Cuts at: 104 2290 2336 3566 104 Size: 2186 46 1230 1286 Fragments arranged by size: 2186 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 350 1762 2540 2730 3590 350 Size: 1412 778 190 860 1508 Fragments arranged by size: 1508 1412 860 778 190 Bsp1286I G_dGCh'C Cuts at: 350 1762 2373 2466 2540 2730 2792 3590 350 Size: 1412 611 93 74 190 62 798 1508 Fragments arranged by size: 1508 1412 798 611 190 93 74 62 BspHI T'CATG_A Cuts at: 882 3180 882 Size: 2298 2450 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1798 2012 2314 2695 1798 Size: 214 302 381 3851 Fragments arranged by size: 3851 381 302 214 BsrBI CCG'CTC Cuts at: 50 3040 3094 4578 4743 50 Size: 2990 54 1484 165 55 Fragments arranged by size: 2990 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 866 1508 2660 866 Size: 642 1152 2954 Fragments arranged by size: 2954 1152 642 BsrFI r'CCGG_y Cuts at: 2746 2927 2746 Size: 181 4567 BssSI C'ACGA_G Cuts at: 3019 3727 3019 Size: 708 4040 BstAPI GCAn_nnn'nTGC Cuts at: 2125 2125 Size: 4748 BstBI TT'CG_AA Cuts at: 3109 3109 Size: 4748 BstXI CCAn_nnnn'nTGG Cuts at: 1497 1497 Size: 4748 BstYI r'GATC_y Cuts at: 1 1059 1773 2240 2263 2598 2844 3150 3162 Size: 1058 714 467 23 335 246 306 12 Cuts at: 3162 3248 4300 1 Size: 86 1052 449 Fragments arranged by size: 1058 1052 714 467 449 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 443 443 Size: 4748 BtgI C'CryG_G Cuts at: 107 1250 1340 107 Size: 1143 90 3515 Fragments arranged by size: 3515 1143 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2698 2879 3042 2698 Size: 181 163 4404 Fragments arranged by size: 4404 181 163 BtsI GCAGTG_nn' Cuts at: 1595 1991 1595 Size: 396 4352 ClaI AT'CG_AT Cuts at: 311 1370 311 Size: 1059 3689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 111 146 1325 1360 111 Size: 35 1179 35 3499 Fragments arranged by size: 3499 1179 35 35 DraI TTT'AAA Cuts at: 1747 1747 Size: 4748 DraIII CAC_nnn'GTG Cuts at: 984 1324 1672 984 Size: 340 348 4060 Fragments arranged by size: 4060 348 340 DrdI GACnn_nn'nnGTC Cuts at: 2454 3798 2454 Size: 1344 3404 EaeI y'GGCC_r Cuts at: 2333 2507 2898 2925 4270 2333 Size: 174 391 27 1345 2811 Fragments arranged by size: 2811 1345 391 174 27 EagI C'GGCC_G Cuts at: 2333 2333 Size: 4748 EarI CTCTTCn'nnn_ Cuts at: 1403 1594 2771 2981 1403 Size: 191 1177 210 3170 Fragments arranged by size: 3170 1177 210 191 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2956 3698 3844 4558 2956 Size: 742 146 714 3146 Fragments arranged by size: 3146 742 714 146 EcoICRI GAG'CTC Cuts at: 348 1760 348 Size: 1412 3336 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 359 401 655 1074 1175 1618 1736 2573 3005 Size: 42 254 419 101 443 118 837 432 Cuts at: 3005 3358 4020 359 Size: 353 662 1087 Fragments arranged by size: 1087 837 662 443 432 419 353 254 118 101 42 EcoNI CCTnn'n_nnAGG Cuts at: 1261 1261 Size: 4748 EcoO109I rG'GnC_Cy Cuts at: 1559 1887 1559 Size: 328 4420 EcoRI G'AATT_C Cuts at: 1752 1752 Size: 4748 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2767 2767 Size: 4748 FspI TGC'GCA Cuts at: 2529 2529 Size: 4748 HaeII r_GCGC'y Cuts at: 198 2182 2430 3660 4431 198 Size: 1984 248 1230 771 515 Fragments arranged by size: 1984 1230 771 515 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 571 603 1318 1350 2590 2620 2622 2652 571 Size: 32 715 32 1240 30 2 30 2667 Fragments arranged by size: 2667 1240 715 32 32 30 30 2 HincII GTy'rAC Cuts at: 249 258 1337 1787 249 Size: 9 1079 450 3210 Fragments arranged by size: 3210 1079 450 9 HindIII A'AGCT_T Cuts at: 722 722 Size: 4748 HpaI GTT'AAC Cuts at: 249 249 Size: 4748 KasI G'GCGC_C Cuts at: 2178 2426 2178 Size: 248 4500 KpnI G_GTAC'C Cuts at: 1768 1768 Size: 4748 MluI A'CGCG_T Cuts at: 4181 4181 Size: 4748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3507 3691 3507 Size: 184 4564 MscI TGG'CCA Cuts at: 2509 2509 Size: 4748 MslI CAynn'nnrTG Cuts at: 887 986 1495 2737 2864 887 Size: 99 509 1242 127 2771 Fragments arranged by size: 2771 1242 509 127 99 MspA1I CmG'CkG Cuts at: 231 553 781 1859 2173 2533 3317 3562 231 Size: 322 228 1078 314 360 784 245 1417 Fragments arranged by size: 1417 1078 784 360 322 314 245 228 NaeI GCC'GGC Cuts at: 2929 2929 Size: 4748 NarI GG'CG_CC Cuts at: 2179 2427 2179 Size: 248 4500 NcoI C'CATG_G Cuts at: 107 1250 1340 107 Size: 1143 90 3515 Fragments arranged by size: 3515 1143 90 NdeI CA'TA_TG Cuts at: 527 527 Size: 4748 NgoMIV G'CCGG_C Cuts at: 2927 2927 Size: 4748 NspI r_CATG'y Cuts at: 478 1104 1801 2301 3904 478 Size: 626 697 500 1603 1322 Fragments arranged by size: 1603 1322 697 626 500 PciI A'CATG_T Cuts at: 1100 3900 1100 Size: 2800 1948 PflMI CCAn_nnn'nTGG Cuts at: 1226 1226 Size: 4748 PfoI T'CCnGG_A Cuts at: 386 1225 386 Size: 839 3909 PmeI GTTT'AAAC Cuts at: 1747 1747 Size: 4748 PmlI CAC'GTG Cuts at: 984 984 Size: 4748 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3194 3226 3194 Size: 32 4716 PpuMI rG'GwC_Cy Cuts at: 1559 1559 Size: 4748 PstI C_TGCA'G Cuts at: 1280 1795 2480 1280 Size: 515 685 3548 Fragments arranged by size: 3548 685 515 PvuI CG_AT'CG Cuts at: 104 104 Size: 4748 PvuII CAG'CTG Cuts at: 231 553 781 2173 2533 231 Size: 322 228 1392 360 2446 Fragments arranged by size: 2446 1392 360 322 228 RsrII CG'GwC_CG Cuts at: 2943 2943 Size: 4748 SacI G_AGCT'C Cuts at: 350 1762 350 Size: 1412 3336 SalI G'TCGA_C Cuts at: 256 1785 256 Size: 1529 3219 SapI GCTCTTCn'nnn_ Cuts at: 2771 2981 2771 Size: 210 4538 SbfI CC_TGCA'GG Cuts at: 1280 1795 1280 Size: 515 4233 ScaI AGT'ACT Cuts at: 4265 4265 Size: 4748 SexAI A'CCwGG_T Cuts at: 1616 1616 Size: 4748 SfcI C'TryA_G Cuts at: 33 1094 1276 1791 2476 3444 3635 33 Size: 1061 182 515 685 968 191 1146 Fragments arranged by size: 1146 1061 968 685 515 191 182 SfoI GGC'GCC Cuts at: 2180 2428 2180 Size: 248 4500 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4748 SmaI CCC'GGG Cuts at: 1770 1770 Size: 4748 SmlI C'TyrA_G Cuts at: 970 1903 2818 3532 3794 970 Size: 933 915 714 262 1924 Fragments arranged by size: 1924 933 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4748 SphI G_CATG'C Cuts at: 478 1801 2301 478 Size: 1323 500 2925 Fragments arranged by size: 2925 1323 500 SspI AAT'ATT Cuts at: 273 273 Size: 4748 StyI C'CwwG_G Cuts at: 107 1250 1340 1882 2236 107 Size: 1143 90 542 354 2619 Fragments arranged by size: 2619 1143 542 354 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1229 2331 2464 2923 3132 1229 Size: 1102 133 459 209 2845 Fragments arranged by size: 2845 1102 459 209 133 TatI w'GTAC_w Cuts at: 746 4263 746 Size: 3517 1231 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 482 514 4265 4297 482 Size: 32 3751 32 933 Fragments arranged by size: 3751 933 32 32 Tth111I GACn'n_nGTC Cuts at: 370 930 2545 370 Size: 560 1615 2573 Fragments arranged by size: 2573 1615 560 XbaI T'CTAG_A Cuts at: 1779 1779 Size: 4748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1223 1257 2243 1223 Size: 34 986 3728 Fragments arranged by size: 3728 986 34 XmaI C'CCGG_G Cuts at: 1768 1768 Size: 4748 XmnI GAAnn'nnTTC Cuts at: 1603 1603 Size: 4748 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I EcoRV FseI FspAI MfeI NheI NotI NruI NsiI PacI PasI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI