Restriction map for
pF1KB3063
(Circular) (Six-base) MAPSORT of: pF1KB3063.seq Check: 9642 from: 1 to: 5258
pF1KB3063 5258 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1037 2296 1037
Size: 1259 3999
Acc65I G'GTAC_C
Cuts at: 2274 2274
Size: 5258
AclI AA'CG_TT
Cuts at: 409 5098 409
Size: 4689 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 659 1060 1678 3083 3515 3868 4530 334
Size: 325 401 618 1405 432 353 662 1062
Fragments arranged by size:
1405 1062 662 618 432 401 353 325
AfeI AGC'GCT
Cuts at: 1178 1346 1178
Size: 168 5090
AflIII A'CryG_T
Cuts at: 193 1769 2610 4410 4691 193
Size: 1576 841 1800 281 760
Fragments arranged by size:
1800 1576 841 760 281
AleI CACnn'nnGTG
Cuts at: 2125 2125
Size: 5258
AlwNI CAG_nnn'CTG
Cuts at: 739 2563 4001 739
Size: 1824 1438 1996
Fragments arranged by size:
1996 1824 1438
ApaLI G'TGCA_C
Cuts at: 962 4096 962
Size: 3134 2124
ApoI r'AATT_y
Cuts at: 238 366 926 2017 2262 4611 4867 238
Size: 128 560 1091 245 2349 256 629
Fragments arranged by size:
2349 1091 629 560 256 245 128
AseI AT'TA_AT
Cuts at: 20 160 431 20
Size: 140 271 4847
Fragments arranged by size:
4847 271 140
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
AvaI C'yCGr_G
Cuts at: 371 1180 2057 2278 371
Size: 809 877 221 3351
Fragments arranged by size:
3351 877 809 221
BamHI G'GATC_C
Cuts at: 2283 2283
Size: 5258
BanI G'GyrC_C
Cuts at: 805 1416 2274 2688 2936 2971 805
Size: 611 858 414 248 35 3092
Fragments arranged by size:
3092 858 611 414 248 35
BanII G_rGCy'C
Cuts at: 797 1710 1851 2272 3302 797
Size: 913 141 421 1030 2753
Fragments arranged by size:
2753 1030 913 421 141
BbeI G_GCGC'C
Cuts at: 1420 2692 2940 1420
Size: 1272 248 3738
Fragments arranged by size:
3738 1272 248
BbsI GAAGACnn'nnnn_
Cuts at: 1658 1689 1658
Size: 31 5227
BbvCI CC'TCA_GC
Cuts at: 772 772
Size: 5258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 931 965 2056 2090 931
Size: 34 1091 34 4099
Fragments arranged by size:
4099 1091 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3156 4212 5258 3156
Size: 1056 1046 3156
Fragments arranged by size:
3156 1056 1046
BclI T'GATC_A
Cuts at: 2778 2778
Size: 5258
BglI GCCn_nnn'nGGC
Cuts at: 2183 2183
Size: 5258
BglII A'GATC_T
Cuts at: 2773 4810 2773
Size: 2037 3221
BlpI GC'TnA_GC
Cuts at: 2370 2370
Size: 5258
Bme1580I G_kGCm'C
Cuts at: 644 808 966 2883 2976 4100 644
Size: 164 158 1917 93 1124 1802
Fragments arranged by size:
1917 1802 1124 164 158 93
BmgBI CAC'GTC
Cuts at: 1016 1770 2070 1016
Size: 754 300 4204
Fragments arranged by size:
4204 754 300
BmrI ACTGGGnnnn_n'
Cuts at: 124 1402 2470 2551 2886 5030 124
Size: 1278 1068 81 335 2144 352
Fragments arranged by size:
2144 1278 1068 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3625 3625
Size: 5258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1541 1663 2188 2202 2217 2232 889
Size: 652 122 525 14 15 15 3915
Fragments arranged by size:
3915 652 525 122 15 15 14
Bpu10I CC'TnA_GC
Cuts at: 772 791 1138 1231 772
Size: 19 347 93 4799
Fragments arranged by size:
4799 347 93 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 2434 3313 4027 4325 730
Size: 1704 879 714 298 1663
Fragments arranged by size:
1704 1663 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 904 1323 1666 2094 4962 904
Size: 419 343 428 2868 1200
Fragments arranged by size:
2868 1200 428 419 343
BsaAI yAC'GTr
Cuts at: 1134 2611 1134
Size: 1477 3781
BsaBI GATnn'nnATC
Cuts at: 6 2772 4809 6
Size: 2766 2037 455
Fragments arranged by size:
2766 2037 455
BsaHI Gr'CG_yC
Cuts at: 1417 2689 2937 3639 5135 1417
Size: 1272 248 702 1496 1540
Fragments arranged by size:
1540 1496 1272 702 248
BsaWI w'CCGG_w
Cuts at: 1625 2447 2968 3646 4057 4204 4478 1625
Size: 822 521 678 411 147 274 2405
Fragments arranged by size:
2405 822 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1195 1225 1320 1350 1567 1597 1949 1979 2221
Size: 30 95 30 217 30 352 30 242
Cuts at: 2221 2251 1195
Size: 30 4202
Fragments arranged by size:
4202 352 242 217 95 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1055 1651 2205 1055
Size: 596 554 4108
Fragments arranged by size:
4108 596 554
BseYI C'CCAG_C
Cuts at: 781 1143 1908 2192 2683 3441 4106 4514 781
Size: 362 765 284 491 758 665 408 1525
Fragments arranged by size:
1525 765 758 665 491 408 362 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1903 1987 133
Size: 1770 84 3404
Fragments arranged by size:
3404 1770 84
BsiEI CG_ry'CG
Cuts at: 104 2800 2846 4076 104
Size: 2696 46 1230 1286
Fragments arranged by size:
2696 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 966 1139 1439 2272 3050 3240 4100 966
Size: 173 300 833 778 190 860 2124
Fragments arranged by size:
2124 860 833 778 300 190 173
Bsp1286I G_dGCh'C
Cuts at: 644 797 808 966 1139 1439 1710 1851 2272
Size: 153 11 158 173 300 271 141 421
Cuts at: 2272 2883 2976 3050 3240 3302 4100 644
Size: 611 93 74 190 62 798 1802
Fragments arranged by size:
1802 798 611 421 300 271 190 173
158 153 141 93 74 62 11
BspHI T'CATG_A
Cuts at: 3690 3690
Size: 5258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 2076 2308 2522 2824 3205 1294
Size: 782 232 214 302 381 3347
Fragments arranged by size:
3347 782 381 302 232 214
BsrBI CCG'CTC
Cuts at: 50 3550 3604 5088 5253 50
Size: 3500 54 1484 165 55
Fragments arranged by size:
3500 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 438 557 3170 438
Size: 119 2613 2526
Fragments arranged by size:
2613 2526 119
BsrFI r'CCGG_y
Cuts at: 3256 3437 3256
Size: 181 5077
BsrGI T'GTAC_A
Cuts at: 140 140
Size: 5258
BssSI C'ACGA_G
Cuts at: 3529 4237 3529
Size: 708 4550
BstAPI GCAn_nnn'nTGC
Cuts at: 2635 2635
Size: 5258
BstBI TT'CG_AA
Cuts at: 3619 3619
Size: 5258
BstXI CCAn_nnnn'nTGG
Cuts at: 1167 1519 1167
Size: 352 4906
BstYI r'GATC_y
Cuts at: 1 2283 2750 2773 3108 3354 3660 3672 3758
Size: 2282 467 23 335 246 306 12 86
Cuts at: 3758 4810 1
Size: 1052 449
Fragments arranged by size:
2282 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1067 1067
Size: 5258
BtgI C'CryG_G
Cuts at: 107 716 797 107
Size: 609 81 4568
Fragments arranged by size:
4568 609 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1251 3208 3389 3552 1251
Size: 1957 181 163 2957
Fragments arranged by size:
2957 1957 181 163
BtsI GCAGTG_nn'
Cuts at: 228 2017 2501 228
Size: 1789 484 2985
Fragments arranged by size:
2985 1789 484
DraI TTT'AAA
Cuts at: 2257 2257
Size: 5258
DrdI GACnn_nn'nnGTC
Cuts at: 2964 4308 2964
Size: 1344 3914
EaeI y'GGCC_r
Cuts at: 719 1990 2843 3017 3408 3435 4780 719
Size: 1271 853 174 391 27 1345 1197
Fragments arranged by size:
1345 1271 1197 853 391 174 27
EagI C'GGCC_G
Cuts at: 2843 2843
Size: 5258
EarI CTCTTCn'nnn_
Cuts at: 678 1238 2097 3281 3491 678
Size: 560 859 1184 210 2445
Fragments arranged by size:
2445 1184 859 560 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 644 3466 4208 4354 5068 644
Size: 2822 742 146 714 834
Fragments arranged by size:
2822 834 742 714 146
EcoICRI GAG'CTC
Cuts at: 2270 2270
Size: 5258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 659 889 1060 1541 1663 1678 2188 2202
Size: 325 230 171 481 122 15 510 14
Cuts at: 2202 2217 2232 3083 3515 3868 4530 334
Size: 15 15 851 432 353 662 1062
Fragments arranged by size:
1062 851 662 510 481 432 353 325
230 171 122 15 15 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 177 748 177
Size: 571 4687
EcoO109I rG'GnC_Cy
Cuts at: 1071 1516 2397 1071
Size: 445 881 3932
Fragments arranged by size:
3932 881 445
EcoRI G'AATT_C
Cuts at: 238 366 2262 238
Size: 128 1896 3234
Fragments arranged by size:
3234 1896 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 5258
FspI TGC'GCA
Cuts at: 3039 3039
Size: 5258
HaeII r_GCGC'y
Cuts at: 282 1180 1348 1420 2692 2940 4170 4941 282
Size: 898 168 72 1272 248 1230 771 599
Fragments arranged by size:
1272 1230 898 771 599 248 168 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 496 528 987 1019 1050 1082 1193 1225 1729
Size: 32 459 32 31 32 111 32 504
Cuts at: 1729 1761 3100 3130 3132 3162 496
Size: 32 1339 30 2 30 2592
Fragments arranged by size:
2592 1339 504 459 111 32 32 32
32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 2297 2297
Size: 5258
KasI G'GCGC_C
Cuts at: 1416 2688 2936 1416
Size: 1272 248 3738
Fragments arranged by size:
3738 1272 248
KpnI G_GTAC'C
Cuts at: 2278 2278
Size: 5258
MluI A'CGCG_T
Cuts at: 4691 4691
Size: 5258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 628 4017 4201 628
Size: 3389 184 1685
Fragments arranged by size:
3389 1685 184
MscI TGG'CCA
Cuts at: 3019 3019
Size: 5258
MslI CAynn'nnrTG
Cuts at: 1363 2125 3247 3374 1363
Size: 762 1122 127 3247
Fragments arranged by size:
3247 1122 762 127
MspA1I CmG'CkG
Cuts at: 867 1908 2183 2196 2369 2683 3043 3827 4072
Size: 1041 275 13 173 314 360 784 245
Cuts at: 4072 867
Size: 2053
Fragments arranged by size:
2053 1041 784 360 314 275 245 173
13
NaeI GCC'GGC
Cuts at: 3439 3439
Size: 5258
NarI GG'CG_CC
Cuts at: 1417 2689 2937 1417
Size: 1272 248 3738
Fragments arranged by size:
3738 1272 248
NcoI C'CATG_G
Cuts at: 107 716 107
Size: 609 4649
NgoMIV G'CCGG_C
Cuts at: 3437 3437
Size: 5258
NspI r_CATG'y
Cuts at: 197 1317 1584 2031 2311 2811 4414 197
Size: 1120 267 447 280 500 1603 1041
Fragments arranged by size:
1603 1120 1041 500 447 280 267
PasI CC'CwG_GG
Cuts at: 1421 1842 1421
Size: 421 4837
PciI A'CATG_T
Cuts at: 193 4410 193
Size: 4217 1041
PflMI CCAn_nnn'nTGG
Cuts at: 1600 1600
Size: 5258
PfoI T'CCnGG_A
Cuts at: 905 1518 905
Size: 613 4645
PmeI GTTT'AAAC
Cuts at: 2257 2257
Size: 5258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 5226
PpuMI rG'GwC_Cy
Cuts at: 1071 1516 1071
Size: 445 4813
PsiI TTA'TAA
Cuts at: 401 401
Size: 5258
PstI C_TGCA'G
Cuts at: 872 1635 2012 2087 2305 2990 872
Size: 763 377 75 218 685 3140
Fragments arranged by size:
3140 763 685 377 218 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5258
PvuII CAG'CTG
Cuts at: 1908 2196 2683 3043 1908
Size: 288 487 360 4123
Fragments arranged by size:
4123 487 360 288
RsrII CG'GwC_CG
Cuts at: 3453 3453
Size: 5258
SacI G_AGCT'C
Cuts at: 2272 2272
Size: 5258
SalI G'TCGA_C
Cuts at: 2295 2295
Size: 5258
SapI GCTCTTCn'nnn_
Cuts at: 3281 3491 3281
Size: 210 5048
SbfI CC_TGCA'GG
Cuts at: 2087 2305 2087
Size: 218 5040
ScaI AGT'ACT
Cuts at: 4775 4775
Size: 5258
SexAI A'CCwGG_T
Cuts at: 1332 1984 1332
Size: 652 4606
SfcI C'TryA_G
Cuts at: 33 868 1631 1920 2008 2083 2301 2986 3954
Size: 835 763 289 88 75 218 685 968
Cuts at: 3954 4145 33
Size: 191 1146
Fragments arranged by size:
1146 968 835 763 685 289 218 191
88 75
SfoI GGC'GCC
Cuts at: 1418 2690 2938 1418
Size: 1272 248 3738
Fragments arranged by size:
3738 1272 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
SmaI CCC'GGG
Cuts at: 2059 2280 2059
Size: 221 5037
SmlI C'TyrA_G
Cuts at: 745 2413 3328 4042 4304 745
Size: 1668 915 714 262 1699
Fragments arranged by size:
1699 1668 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5258
SphI G_CATG'C
Cuts at: 2031 2311 2811 2031
Size: 280 500 4478
Fragments arranged by size:
4478 500 280
SspI AAT'ATT
Cuts at: 978 978
Size: 5258
StuI AGG'CCT
Cuts at: 1526 1526
Size: 5258
StyI C'CwwG_G
Cuts at: 107 716 2392 2746 107
Size: 609 1676 354 2619
Fragments arranged by size:
2619 1676 609 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 318 1842 2841 2974 3433 3642 318
Size: 1524 999 133 459 209 1934
Fragments arranged by size:
1934 1524 999 459 209 133
TatI w'GTAC_w
Cuts at: 140 532 2114 4773 140
Size: 392 1582 2659 625
Fragments arranged by size:
2659 1582 625 392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1320 1352 1567 1599 1949 1981 2221 2253 4775
Size: 32 215 32 350 32 240 32 2522
Cuts at: 4775 4807 1320
Size: 32 1771
Fragments arranged by size:
2522 1771 350 240 215 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 3055 1897
Size: 1158 4100
XbaI T'CTAG_A
Cuts at: 2289 2289
Size: 5258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2163 2753 2163
Size: 590 4668
XmaI C'CCGG_G
Cuts at: 2057 2278 2057
Size: 221 5037
XmnI GAAnn'nnTTC
Cuts at: 238 238
Size: 5258
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BsmBI
BspEI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI