Restriction map for
pF1KB3011
(Circular) (Six-base) MAPSORT of: pF1KB3011.seq Check: 7927 from: 1 to: 5276
pF1KB3011 5276 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 796 796
Size: 5276
AccI GT'mk_AC
Cuts at: 221 2314 221
Size: 2093 3183
Acc65I G'GTAC_C
Cuts at: 2292 2292
Size: 5276
AclI AA'CG_TT
Cuts at: 2270 5116 2270
Size: 2846 2430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 695 3101 3533 3886 4548 628
Size: 67 2406 432 353 662 1356
Fragments arranged by size:
2406 1356 662 432 353 67
AfeI AGC'GCT
Cuts at: 1625 1625
Size: 5276
AflII C'TTAA_G
Cuts at: 2176 2176
Size: 5276
AflIII A'CryG_T
Cuts at: 749 2628 4428 4709 749
Size: 1879 1800 281 1316
Fragments arranged by size:
1879 1800 1316 281
AlwNI CAG_nnn'CTG
Cuts at: 1647 2581 4019 1647
Size: 934 1438 2904
Fragments arranged by size:
2904 1438 934
ApaI G_GGCC'C
Cuts at: 475 475
Size: 5276
ApaLI G'TGCA_C
Cuts at: 809 4114 809
Size: 3305 1971
ApoI r'AATT_y
Cuts at: 118 992 2228 2280 4629 4885 118
Size: 874 1236 52 2349 256 509
Fragments arranged by size:
2349 1236 874 509 256 52
AseI AT'TA_AT
Cuts at: 20 587 20
Size: 567 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5276
AvaI C'yCGr_G
Cuts at: 2128 2296 2128
Size: 168 5108
BamHI G'GATC_C
Cuts at: 642 1341 2301 642
Size: 699 960 3617
Fragments arranged by size:
3617 960 699
BanI G'GyrC_C
Cuts at: 2292 2706 2954 2989 2292
Size: 414 248 35 4579
Fragments arranged by size:
4579 414 248 35
BanII G_rGCy'C
Cuts at: 397 475 527 1260 2290 3320 397
Size: 78 52 733 1030 1030 2353
Fragments arranged by size:
2353 1030 1030 733 78 52
BbeI G_GCGC'C
Cuts at: 2710 2958 2710
Size: 248 5028
BbsI GAAGACnn'nnnn_
Cuts at: 324 602 1871 324
Size: 278 1269 3729
Fragments arranged by size:
3729 1269 278
BciVI GTATCCnnnnn_n'
Cuts at: 3174 4230 5276 3174
Size: 1056 1046 3174
Fragments arranged by size:
3174 1056 1046
BclI T'GATC_A
Cuts at: 2796 2796
Size: 5276
BglI GCCn_nnn'nGGC
Cuts at: 1380 1380
Size: 5276
BglII A'GATC_T
Cuts at: 561 700 1530 2791 4828 561
Size: 139 830 1261 2037 1009
Fragments arranged by size:
2037 1261 1009 830 139
BlpI GC'TnA_GC
Cuts at: 2388 2388
Size: 5276
Bme1580I G_kGCm'C
Cuts at: 475 813 2901 2994 4118 475
Size: 338 2088 93 1124 1633
Fragments arranged by size:
2088 1633 1124 338 93
BmgBI CAC'GTC
Cuts at: 709 709
Size: 5276
BmrI ACTGGGnnnn_n'
Cuts at: 359 1176 2488 2569 2904 5048 359
Size: 817 1312 81 335 2144 587
Fragments arranged by size:
2144 1312 817 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 5276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1357 1441 484
Size: 873 84 4319
Fragments arranged by size:
4319 873 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 2452 3331 4045 4343 511
Size: 1941 879 714 298 1444
Fragments arranged by size:
1941 1444 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4980 4980
Size: 5276
BsaAI yAC'GTr
Cuts at: 298 2629 298
Size: 2331 2945
BsaBI GATnn'nnATC
Cuts at: 6 1319 2790 4827 6
Size: 1313 1471 2037 455
Fragments arranged by size:
2037 1471 1313 455
BsaHI Gr'CG_yC
Cuts at: 2707 2955 3657 5153 2707
Size: 248 702 1496 2830
Fragments arranged by size:
2830 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2465 2986 3664 4075 4222 4496 2465
Size: 521 678 411 147 274 3245
Fragments arranged by size:
3245 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1476 1506 1476
Size: 30 5246
BseYI C'CCAG_C
Cuts at: 1724 2701 3459 4124 4532 1724
Size: 977 758 665 408 2468
Fragments arranged by size:
2468 977 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 5276
BsiEI CG_ry'CG
Cuts at: 104 2818 2864 4094 104
Size: 2714 46 1230 1286
Fragments arranged by size:
2714 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 813 1260 2290 3068 3258 4118 813
Size: 447 1030 778 190 860 1971
Fragments arranged by size:
1971 1030 860 778 447 190
BsmI GAATG_Cn'
Cuts at: 2252 2252
Size: 5276
Bsp1286I G_dGCh'C
Cuts at: 397 475 527 813 1260 2290 2901 2994 3068
Size: 78 52 286 447 1030 611 93 74
Cuts at: 3068 3258 3320 4118 397
Size: 190 62 798 1555
Fragments arranged by size:
1555 1030 798 611 447 286 190 93
78 74 62 52
BspHI T'CATG_A
Cuts at: 131 1179 1398 3708 131
Size: 1048 219 2310 1699
Fragments arranged by size:
2310 1699 1048 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 2326 2540 2842 3223 796
Size: 1530 214 302 381 2849
Fragments arranged by size:
2849 1530 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3568 3622 5106 5271 50
Size: 3518 54 1484 165 55
Fragments arranged by size:
3518 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 204 3188 204
Size: 2984 2292
BsrFI r'CCGG_y
Cuts at: 3274 3455 3274
Size: 181 5095
BsrGI T'GTAC_A
Cuts at: 858 1212 858
Size: 354 4922
BssSI C'ACGA_G
Cuts at: 3547 4255 3547
Size: 708 4568
BstAPI GCAn_nnn'nTGC
Cuts at: 2653 2653
Size: 5276
BstBI TT'CG_AA
Cuts at: 3637 3637
Size: 5276
BstEII G'GTnAC_C
Cuts at: 2168 2168
Size: 5276
BstYI r'GATC_y
Cuts at: 1 561 642 700 1341 1530 2301 2768 2791
Size: 560 81 58 641 189 771 467 23
Cuts at: 2791 3126 3372 3678 3690 3776 4828 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 771 641 560 467 449 335 306
246 189 86 81 58 23 12
BtgI C'CryG_G
Cuts at: 107 1010 107
Size: 903 4373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3226 3407 3570 3226
Size: 181 163 4932
Fragments arranged by size:
4932 181 163
BtsI GCAGTG_nn'
Cuts at: 2519 2519
Size: 5276
DraI TTT'AAA
Cuts at: 2275 2275
Size: 5276
DrdI GACnn_nn'nnGTC
Cuts at: 2982 4326 2982
Size: 1344 3932
EaeI y'GGCC_r
Cuts at: 2861 3035 3426 3453 4798 2861
Size: 174 391 27 1345 3339
Fragments arranged by size:
3339 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2861 2861
Size: 5276
EarI CTCTTCn'nnn_
Cuts at: 479 3299 3509 479
Size: 2820 210 2246
Fragments arranged by size:
2820 2246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3484 4226 4372 5086 3484
Size: 742 146 714 3674
Fragments arranged by size:
3674 742 714 146
EcoICRI GAG'CTC
Cuts at: 1258 2288 1258
Size: 1030 4246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 628 695 1357 1441 3101 3533 3886 4548
Size: 144 67 662 84 1660 432 353 662
Cuts at: 4548 484
Size: 1212
Fragments arranged by size:
1660 1212 662 662 432 353 144 84
67
EcoO109I rG'GnC_Cy
Cuts at: 471 472 2415 471
Size: 1 1943 3332
Fragments arranged by size:
3332 1943 1
EcoRI G'AATT_C
Cuts at: 2280 2280
Size: 5276
EcoRV GAT'ATC
Cuts at: 1805 1805
Size: 5276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1115 1261 3295 1115
Size: 146 2034 3096
Fragments arranged by size:
3096 2034 146
FspI TGC'GCA
Cuts at: 3057 3057
Size: 5276
HaeII r_GCGC'y
Cuts at: 1627 2710 2958 4188 4959 1627
Size: 1083 248 1230 771 1944
Fragments arranged by size:
1944 1230 1083 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 233 265 345 377 379 411 627 659 1621
Size: 32 80 32 2 32 216 32 962
Cuts at: 1621 1653 3118 3148 3150 3180 233
Size: 32 1465 30 2 30 2329
Fragments arranged by size:
2329 1465 962 216 80 32 32 32
32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 1022 1500 2315 1022
Size: 478 815 3983
Fragments arranged by size:
3983 815 478
HindIII A'AGCT_T
Cuts at: 1519 2059 1519
Size: 540 4736
HpaI GTT'AAC
Cuts at: 1022 1500 1022
Size: 478 4798
KasI G'GCGC_C
Cuts at: 2706 2954 2706
Size: 248 5028
KpnI G_GTAC'C
Cuts at: 2296 2296
Size: 5276
MfeI C'AATT_G
Cuts at: 1539 1539
Size: 5276
MluI A'CGCG_T
Cuts at: 4709 4709
Size: 5276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4035 4219 4035
Size: 184 5092
MscI TGG'CCA
Cuts at: 3037 3037
Size: 5276
MslI CAynn'nnrTG
Cuts at: 748 1508 3265 3392 748
Size: 760 1757 127 2632
Fragments arranged by size:
2632 1757 760 127
MspA1I CmG'CkG
Cuts at: 513 834 948 2387 2701 3061 3845 4090 513
Size: 321 114 1439 314 360 784 245 1699
Fragments arranged by size:
1699 1439 784 360 321 314 245 114
NaeI GCC'GGC
Cuts at: 3457 3457
Size: 5276
NarI GG'CG_CC
Cuts at: 2707 2955 2707
Size: 248 5028
NcoI C'CATG_G
Cuts at: 107 1010 107
Size: 903 4373
NgoMIV G'CCGG_C
Cuts at: 3455 3455
Size: 5276
NspI r_CATG'y
Cuts at: 292 493 753 2329 2829 4432 292
Size: 201 260 1576 500 1603 1136
Fragments arranged by size:
1603 1576 1136 500 260 201
PciI A'CATG_T
Cuts at: 749 4428 749
Size: 3679 1597
PfoI T'CCnGG_A
Cuts at: 954 983 954
Size: 29 5247
PmeI GTTT'AAAC
Cuts at: 2275 2275
Size: 5276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 5244
PshAI GACnn'nnGTC
Cuts at: 623 623
Size: 5276
PsiI TTA'TAA
Cuts at: 1163 1163
Size: 5276
PspOMI G'GGCC_C
Cuts at: 471 471
Size: 5276
PspXI vC'TCGA_Gb
Cuts at: 2128 2128
Size: 5276
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 403 435 403
Size: 32 5244
PstI C_TGCA'G
Cuts at: 762 2323 3008 762
Size: 1561 685 3030
Fragments arranged by size:
3030 1561 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5276
PvuII CAG'CTG
Cuts at: 513 834 948 2701 3061 513
Size: 321 114 1753 360 2728
Fragments arranged by size:
2728 1753 360 321 114
RsrII CG'GwC_CG
Cuts at: 3471 3471
Size: 5276
SacI G_AGCT'C
Cuts at: 1260 2290 1260
Size: 1030 4246
SalI G'TCGA_C
Cuts at: 2313 2313
Size: 5276
SapI GCTCTTCn'nnn_
Cuts at: 479 3299 3509 479
Size: 2820 210 2246
Fragments arranged by size:
2820 2246 210
SbfI CC_TGCA'GG
Cuts at: 2323 2323
Size: 5276
ScaI AGT'ACT
Cuts at: 4793 4793
Size: 5276
SfcI C'TryA_G
Cuts at: 33 218 758 2319 3004 3972 4163 33
Size: 185 540 1561 685 968 191 1146
Fragments arranged by size:
1561 1146 968 685 540 191 185
SfoI GGC'GCC
Cuts at: 2708 2956 2708
Size: 248 5028
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5276
SmaI CCC'GGG
Cuts at: 2298 2298
Size: 5276
SmlI C'TyrA_G
Cuts at: 526 2128 2176 2431 3346 4060 4322 526
Size: 1602 48 255 915 714 262 1480
Fragments arranged by size:
1602 1480 915 714 262 255 48
SpeI A'CTAG_T
Cuts at: 67 1984 67
Size: 1917 3359
SphI G_CATG'C
Cuts at: 493 2329 2829 493
Size: 1836 500 2940
Fragments arranged by size:
2940 1836 500
SspI AAT'ATT
Cuts at: 1309 1411 1309
Size: 102 5174
StyI C'CwwG_G
Cuts at: 107 1010 2410 2764 107
Size: 903 1400 354 2619
Fragments arranged by size:
2619 1400 903 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2859 2992 3451 3660 2859
Size: 133 459 209 4475
Fragments arranged by size:
4475 459 209 133
TatI w'GTAC_w
Cuts at: 434 549 858 1212 1287 4791 434
Size: 115 309 354 75 3504 919
Fragments arranged by size:
3504 919 354 309 115 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 965 997 1476 1508 4793 4825 965
Size: 32 479 32 3285 32 1416
Fragments arranged by size:
3285 1416 479 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3073 3073
Size: 5276
XbaI T'CTAG_A
Cuts at: 2307 2307
Size: 5276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1900 2771 1900
Size: 871 4405
XhoI C'TCGA_G
Cuts at: 2128 2128
Size: 5276
XmaI C'CCGG_G
Cuts at: 2296 2296
Size: 5276
XmnI GAAnn'nnTTC
Cuts at: 848 2087 848
Size: 1239 4037
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BseRI BsiWI
BsmBI BspEI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI ZraI