Restriction map for pF1KB2507
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FauI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MslI        MspA1I      MspI        MunI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII        1066  CGCG                 CG^CG
AciI         1065  CCGC                 CCGC(-3/-1)
AspHI         247  GTGCTC               G[AT]GC[AT]^C
BalI          716  TGGCCA               TGG^CCA
BbsI           37  GTCTTC               GAAGAC(2/6)
BclI          818  TGATCA               T^GATCA
BmyI          247  GTGCTC               G[AGT]GC[ACT]^C
BpmI         1122  CTGGAG               CTGGAG(16/14)
BpuAI          37  GTCTTC               GAAGAC(2/6)
BsaAI         517  TACGTA               [CT]AC^GT[GA]
BsiHKAI       247  GTGCTC               G[AT]GC[AT]^C
BsmAI         157  GTCTC                GTCTC(1/5)
BsmFI        1106  GGGAC                GGGAC(10/14)
BsmI          883  GAATGC               GAATGC(1/-1)
Bsp1286I      247  GTGCTC               G[AGT]GC[ACT]^C
Bsp1407I      258  TGTACA               T^GTACA
BspHI         267  TCATGA               T^CATGA
BspMI         555  GCAGGT               ACCTGC(4/8)
BsrGI         258  TGTACA               T^GTACA
BstUI        1066  CGCG                 CG^CG
BstYI         186  AGATCC               [GA]^GATC[CT]
Cac8I         143  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI            4  GCGC                 GCG^C
DraII        1106  GGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI          624  GACAGGAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     1106  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        20  CCTTGG               C^C[AT][AT]GG
EcoT22I        73  ATGCAT               ATGCA^T
FbaI          818  TGATCA               T^GATCA
HaeII           3  GGCGCT               [GA]GCGC^[CT]
HhaI            4  GCGC                 GCG^C
HinP1I          4  GCGC                 G^CGC
HindIII       298  AAGCTT               A^AGCTT
HphI          471  GGTGA                GGTGA(8/7)
MaeII         518  ACGT                 A^CGT
MflI          186  AGATCC               [GA]^GATC[CT]
MluNI         716  TGGCCA               TGG^CCA
MscI          716  TGGCCA               TGG^CCA
MvnI         1066  CGCG                 CG^CG
NsiI           73  ATGCAT               ATGCA^T
NspI          845  ACATGC               [GA]CATG^[CT]
PpuMI        1106  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          267  TCATGA               T^CATGA
SfcI         1137  CTACAG               C^T[GA][CT]AG
SmlI          761  CTCAAG               C^T[CT][GA]AG
SnaBI         517  TACGTA               TAC^GTA
SspBI         258  TGTACA               T^GTACA
StyI           20  CCTTGG               C^C[AT][AT]GG
Tsp45I        622  GTGAC                ^GT[GC]AC
XcmI         1111  CCAACTATAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         186  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AseI          361  ATTAAT               AT^TAAT
              373  ATTAAT              
AsnI          361  ATTAAT               AT^TAAT
              373  ATTAAT              
BfaI          329  CTAG                 C^TAG
             1174  CTAG                
DraIII        922  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1020  CACACTGTG           
EaeI           32  TGGCCG               [CT]^GGCC[GA]
              716  TGGCCA              
EarI           52  CTCTTC               CTCTTC(1/4)
              161  CTCTTC              
HinfI         474  GAATC                G^A[ACGT]TC
             1069  GAATC               
Ksp632I        52  CTCTTC               CTCTTC(1/4)
              161  CTCTTC              
MaeI          329  CTAG                 C^TAG
             1174  CTAG                
MaeIII        604  GTAAC                ^GT[ACGT]AC
              622  GTGAC               
NlaIV        1106  GGGACC               GG[ACGT]^[ACGT]CC
             1107  GGACCC              
PshBI         361  ATTAAT               AT^TAAT
              373  ATTAAT              
SfaNI          72  GATGC                GCATC(5/9)
              325  GATGC               
TfiI          474  GAATC                G^A[AT]TC
             1069  GAATC               
TspRI         173  CACTG                CA[GC]TG[ACGT][ACGT]^
             1022  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AfaI        AluI        AlwI        AvaII       BbvI        
BsaJI       BsiYI       BslI        BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
ItaI        MboI        MboII       MnlI        MseI        
MvaI        NdeII       NlaIII      RsaI        Sau3AI      
Sau96I      ScrFI       Tru9I       TseI        Tsp509I     
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