Restriction map for pF1KB1569
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglI        BglII       BlnI        BsaHI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraIII      EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        KasI        KspI        MluI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        645  GGTACC               G^GTACC
AccII        2303  CGCG                 CG^CG
AccIII       2645  TCCGGA               T^CCGGA
AhdI          655  GACTCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I        61  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       645  GGTACC               G^GTACC
AspEI         655  GACTCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1949  TGGCCA               TGG^CCA
BanI          645  GGTACC               G^G[CT][GA]CC
BbsI         1807  GAAGAC               GAAGAC(2/6)
BlpI         1251  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1251  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1807  GAAGAC               GAAGAC(2/6)
BsaWI        2645  TCCGGA               [AT]^CCGG[AT]
BseAI        2645  TCCGGA               T^CCGGA
BsmI          323  GCATTC               GAATGC(1/-1)
BspEI        2645  TCCGGA               T^CCGGA
Bst1107I      589  GTATAC               GTA^TAC
BstUI        2303  CGCG                 CG^CG
BstZ17I       589  GTATAC               GTA^TAC
CelII        1251  GCTGAGC              GC^T[ACGT]AGC
DraI         1761  TTTAAA               TTT^AAA
DrdI         1405  GACTACGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1949  TGGCCA               [CT]^GGCC[GA]
Eam1105I      655  GACTCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          157  GAAGAG               CTCTTC(1/4)
EcoNI         666  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2725  GATATC               GAT^ATC
FauI          870  CCCGC                CCCGC(4/6)
KpnI          645  GGTACC               GGTAC^C
Ksp632I       157  GAAGAG               CTCTTC(1/4)
MfeI          237  CAATTG               C^AATTG
MluNI        1949  TGGCCA               TGG^CCA
MroI         2645  TCCGGA               T^CCGGA
MscI         1949  TGGCCA               TGG^CCA
MunI          237  CAATTG               C^AATTG
MvnI         2303  CGCG                 CG^CG
PshAI         223  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI          818  GTCGAC               G^TCGAC
SpeI         1582  ACTAGT               A^CTAGT
XbaI         2913  TCTAGA               T^CTAGA
XcmI          142  CCATCACATGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           61  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          589  GTATAC               GT^[AC][GT]AC
              818  GTCGAC              
AcsI          400  AAATTT               [GA]^AATT[CT]
             1891  GAATTT              
ApoI          400  AAATTT               [GA]^AATT[CT]
             1891  GAATTT              
AspHI         292  GAGCTC               G[AT]GC[AT]^C
             1254  GAGCTC              
BclI          387  TGATCA               T^GATCA
             2258  TGATCA              
BcnI          933  CCGGG                CC^[GC]GG
             2653  CCCGG               
BsaAI        1513  TACGTA               [CT]AC^GT[GA]
             2132  TACGTA              
BsaBI         382  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2355  GATCCCAATC          
BsgI         1905  GTGCAG               GTGCAG(16/14)
             2678  GTGCAG              
BsiHKAI       292  GAGCTC               G[AT]GC[AT]^C
             1254  GAGCTC              
BspHI        1628  TCATGA               T^CATGA
             1827  TCATGA              
BspMI         911  GCAGGT               ACCTGC(4/8)
             1885  GCAGGT              
BstXI        1838  CCAAGGAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2359  CCAATCAGGTGG        
DraII         257  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              513  AGGTCCT             
EcoO109I      257  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              513  AGGTCCT             
FbaI          387  TGATCA               T^GATCA
             2258  TGATCA              
HindIII       812  AAGCTT               A^AGCTT
             2638  AAGCTT              
MamI          382  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2355  GATCCCAATC          
NciI          933  CCGGG                CC^[GC]GG
             2653  CCCGG               
NdeI         1083  CATATG               CA^TATG
             1802  CATATG              
PflMI         142  CCATCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              660  CCAGTCCCTGG         
PpuMI         257  GGGTCCC              [GA]G^G[AT]CC[CT]
              513  AGGTCCT             
PstI          729  CTGCAG               CTGCA^G
              909  CTGCAG              
RcaI         1628  TCATGA               T^CATGA
             1827  TCATGA              
SacI          292  GAGCTC               GAGCT^C
             1254  GAGCTC              
SexAI         203  ACCAGGT              A^CC[AT]GGT
              822  ACCAGGT             
SmlI          295  CTCAAG               C^T[CT][GA]AG
             1495  CTCAAG              
SnaBI        1513  TACGTA               TAC^GTA
             2132  TACGTA              
Van91I        142  CCATCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              660  CCAGTCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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