Restriction map for pF1KB1569
 (Linear) (Six-base) MAPSORT of: KIBB1569.seq  Check: 4133  from: 1  to: 2949



KIBB1569.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     902    1876    2949 
   Size:        902     974    1073

  Fragments arranged by size: 

                1073     974     902
 
AccI GT'mk_AC

Cuts at:      0     590     819    2949 
   Size:        590     229    2130

  Fragments arranged by size: 

                2130     590     229
 
Acc65I G'GTAC_C

Cuts at:      0     645    2949 
   Size:        645    2304
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    2683    2949 
   Size:        505    2178     266

  Fragments arranged by size: 

                2178     505     266
 
AhdI GACnn_n'nnGTC

Cuts at:      0     660    2949 
   Size:        660    2289
 
AleI CACnn'nnGTG

Cuts at:      0     150    1177    2800    2949 
   Size:        150    1027    1623     149

  Fragments arranged by size: 

                1623    1027     150     149
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     283     315    2949 
   Size:        283      32    2634

  Fragments arranged by size: 

                2634     283      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     666    1543    2329    2949 
   Size:        666     877     786     620

  Fragments arranged by size: 

                 877     786     666     620
 
ApoI r'AATT_y

Cuts at:      0     400    1891    2949 
   Size:        400    1491    1058

  Fragments arranged by size: 

                1491    1058     400
 
BanI G'GyrC_C

Cuts at:      0     645    2949 
   Size:        645    2304
 
BanII G_rGCy'C

Cuts at:      0     296    1258    1533    2949 
   Size:        296     962     275    1416

  Fragments arranged by size: 

                1416     962     296     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1814    2949 
   Size:       1814    1135
 
BclI T'GATC_A

Cuts at:      0     387    2258    2949 
   Size:        387    1871     691

  Fragments arranged by size: 

                1871     691     387
 
BlpI GC'TnA_GC

Cuts at:      0    1252    2949 
   Size:       1252    1697
 
Bme1580I G_kGCm'C

Cuts at:      0     607     871    2949 
   Size:        607     264    2078

  Fragments arranged by size: 

                2078     607     264
 
BmgBI CAC'GTC

Cuts at:      0    2405    2949 
   Size:       2405     544
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     654    2142    2484    2932    2949 
   Size:        264     390    1488     342     448      17

  Fragments arranged by size: 

                1488     448     390     342     264      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1272    2949 
   Size:       1272    1677
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1053    1329    2332    2949 
   Size:       1053     276    1003     617

  Fragments arranged by size: 

                1053    1003     617     276
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1480    2949 
   Size:        280    1200    1469

  Fragments arranged by size: 

                1469    1200     280
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1006    1780    2228    2460    2949 
   Size:       1006     774     448     232     489

  Fragments arranged by size: 

                1006     774     489     448     232
 
BsaAI yAC'GTr

Cuts at:      0    1515    2134    2949 
   Size:       1515     619     815

  Fragments arranged by size: 

                1515     815     619
 
BsaBI GATnn'nnATC

Cuts at:      0     386    2359    2949 
   Size:        386    1973     590

  Fragments arranged by size: 

                1973     590     386
 
BsaWI w'CCGG_w

Cuts at:      0    2645    2949 
   Size:       2645     304
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     283     313     957     987    1582    1612    1686    1716 
   Size:        283      30     644      30     595      30      74      30

Cuts at:   1716    2437    2467    2949 
   Size:        721      30     482

  Fragments arranged by size: 

                 721     644     595     482     283      74      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      13    1654    2004    2360    2949 
   Size:         13    1641     350     356     589

  Fragments arranged by size: 

                1641     589     356     350      13
 
BseYI C'CCAG_C

Cuts at:      0     123    2547    2949 
   Size:        123    2424     402

  Fragments arranged by size: 

                2424     402     123
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1926    2699    2949 
   Size:       1926     773     250

  Fragments arranged by size: 

                1926     773     250
 
BsiHKAI G_wGCw'C

Cuts at:      0     296    1258    2949 
   Size:        296     962    1691

  Fragments arranged by size: 

                1691     962     296
 
BsmI GAATG_Cn'

Cuts at:      0     323    2949 
   Size:        323    2626
 
Bsp1286I G_dGCh'C

Cuts at:      0     296     607     871    1258    1533    2949 
   Size:        296     311     264     387     275    1416

  Fragments arranged by size: 

                1416     387     311     296     275     264
 
BspEI T'CCGG_A

Cuts at:      0    2645    2949 
   Size:       2645     304
 
BspHI T'CATG_A

Cuts at:      0    1628    1827    2949 
   Size:       1628     199    1122

  Fragments arranged by size: 

                1628    1122     199
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     902    1876    2949 
   Size:        902     974    1073

  Fragments arranged by size: 

                1073     974     902
 
BsrDI GCAATG_nn'

Cuts at:      0     219     434     783    1758    2724    2949 
   Size:        219     215     349     975     966     225

  Fragments arranged by size: 

                 975     966     349     225     219     215
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1845    2366    2949 
   Size:       1845     521     583

  Fragments arranged by size: 

                1845     583     521
 
BstYI r'GATC_y

Cuts at:      0     131     465     711    2354    2949 
   Size:        131     334     246    1643     595

  Fragments arranged by size: 

                1643     595     334     246     131
 
BstZ17I GTA'TAC

Cuts at:      0     591    2949 
   Size:        591    2358
 
BtgI C'CryG_G

Cuts at:      0     650     956    1175    2537    2870    2949 
   Size:        650     306     219    1362     333      79

  Fragments arranged by size: 

                1362     650     333     306     219      79
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2429    2949 
   Size:       2429     520
 
BtsI GCAGTG_nn'

Cuts at:      0    1914    2778    2949 
   Size:       1914     864     171

  Fragments arranged by size: 

                1914     864     171
 
DraI TTT'AAA

Cuts at:      0    1763    2949 
   Size:       1763    1186
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1411    2949 
   Size:       1411    1538
 
EaeI y'GGCC_r

Cuts at:      0    1949    2949 
   Size:       1949    1000
 
EarI CTCTTCn'nnn_

Cuts at:      0     152    2949 
   Size:        152    2797
 
EcoICRI GAG'CTC

Cuts at:      0     294    1256    2949 
   Size:        294     962    1693

  Fragments arranged by size: 

                1693     962     294
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    1053    1329    2332    2683    2949 
   Size:        505     548     276    1003     351     266

  Fragments arranged by size: 

                1003     548     505     351     276     266
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     670    2949 
   Size:        670    2279
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258     514    2949 
   Size:        258     256    2435

  Fragments arranged by size: 

                2435     258     256
 
EcoRV GAT'ATC

Cuts at:      0    2727    2949 
   Size:       2727     222
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2808    2949 
   Size:       2808     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     123     155     646     678     937     969    1327    1359 
   Size:        123      32     491      32     259      32     358      32

Cuts at:   1359    1582    1614    2339    2371    2437    2469    2472    2504 
   Size:        223      32     725      32      66      32       3      32

Cuts at:   2504    2949 
   Size:        445

  Fragments arranged by size: 

                 725     491     445     358     259     223     123      66
                  32      32      32      32      32      32      32      32
                   3
 
HincII GTy'rAC

Cuts at:      0     522     693     820    1791    2949 
   Size:        522     171     127     971    1158

  Fragments arranged by size: 

                1158     971     522     171     127
 
HindIII A'AGCT_T

Cuts at:      0     812    2638    2949 
   Size:        812    1826     311

  Fragments arranged by size: 

                1826     812     311
 
KpnI G_GTAC'C

Cuts at:      0     649    2949 
   Size:        649    2300
 
MfeI C'AATT_G

Cuts at:      0     237    2949 
   Size:        237    2712
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1860    2949 
   Size:       1860    1089
 
MscI TGG'CCA

Cuts at:      0    1951    2949 
   Size:       1951     998
 
MslI CAynn'nnrTG

Cuts at:      0     150     460    1177    1388    1802    2800    2949 
   Size:        150     310     717     211     414     998     149

  Fragments arranged by size: 

                 998     717     414     310     211     150     149
 
MspA1I CmG'CkG

Cuts at:      0      35     442    1543    1645    2551    2565    2949 
   Size:         35     407    1101     102     906      14     384

  Fragments arranged by size: 

                1101     906     407     384     102      35      14
 
NcoI C'CATG_G

Cuts at:      0     650     956    2537    2949 
   Size:        650     306    1581     412

  Fragments arranged by size: 

                1581     650     412     306
 
NdeI CA'TA_TG

Cuts at:      0    1084    1803    2949 
   Size:       1084     719    1146

  Fragments arranged by size: 

                1146    1084     719
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     148     666    2949 
   Size:        148     518    2283

  Fragments arranged by size: 

                2283     518     148
 
PfoI T'CCnGG_A

Cuts at:      0    1025    2309    2949 
   Size:       1025    1284     640

  Fragments arranged by size: 

                1284    1025     640
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315    2949 
   Size:        283      32    2634

  Fragments arranged by size: 

                2634     283      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     258     514    2949 
   Size:        258     256    2435

  Fragments arranged by size: 

                2435     258     256
 
PshAI GACnn'nnGTC

Cuts at:      0     227    2949 
   Size:        227    2722
 
PstI C_TGCA'G

Cuts at:      0     733     913    2949 
   Size:        733     180    2036

  Fragments arranged by size: 

                2036     733     180
 
PvuII CAG'CTG

Cuts at:      0      35     442    2551    2565    2949 
   Size:         35     407    2109      14     384

  Fragments arranged by size: 

                2109     407     384      35      14
 
SacI G_AGCT'C

Cuts at:      0     296    1258    2949 
   Size:        296     962    1691

  Fragments arranged by size: 

                1691     962     296
 
SalI G'TCGA_C

Cuts at:      0     818    2949 
   Size:        818    2131
 
SanDI GG'GwC_CC

Cuts at:      0     258    2949 
   Size:        258    2691
 
SexAI A'CCwGG_T

Cuts at:      0     203     822    2949 
   Size:        203     619    2127

  Fragments arranged by size: 

                2127     619     203
 
SfcI C'TryA_G

Cuts at:      0     729     909    2384    2507    2949 
   Size:        729     180    1475     123     442

  Fragments arranged by size: 

                1475     729     442     180     123
 
SmlI C'TyrA_G

Cuts at:      0     295    1495    2949 
   Size:        295    1200    1454

  Fragments arranged by size: 

                1454    1200     295
 
SnaBI TAC'GTA

Cuts at:      0    1515    2134    2949 
   Size:       1515     619     815

  Fragments arranged by size: 

                1515     815     619
 
SpeI A'CTAG_T

Cuts at:      0    1582    2949 
   Size:       1582    1367
 
StyI C'CwwG_G

Cuts at:      0     572     650     718     956    1838    2117    2537    2949 
   Size:        572      78      68     238     882     279     420     412

  Fragments arranged by size: 

                 882     572     420     412     279     238      78      68
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     140     204    1088    2949 
   Size:        140      64     884    1861

  Fragments arranged by size: 

                1861     884     140      64
 
TatI w'GTAC_w

Cuts at:      0     365     725     733    2807    2949 
   Size:        365     360       8    2074     142

  Fragments arranged by size: 

                2074     365     360     142       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1686    1718    2949 
   Size:       1686      32    1231

  Fragments arranged by size: 

                1686    1231      32
 
XbaI T'CTAG_A

Cuts at:      0    2913    2949 
   Size:       2913      36
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     149    2949 
   Size:        149    2800
 
XmnI GAAnn'nnTTC

Cuts at:      0      65    2949 
   Size:         65    2884
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI     AloI    AlwNI
     ApoI     BanI    BanII     BbsI     BclI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI    PflMI     PfoI
     PpiI    PpuMI    PshAI     PstI    PvuII     SacI     SalI    SanDI
    SexAI     SfcI     SmlI    SnaBI     SpeI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AlfI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BmtI
   Bpu10I    BsaHI    BsiEI    BsiWI    BsmBI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I     ClaI    CspCI
   DraIII     EagI     EciI    EcoRI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XhoI     XmaI     ZraI
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